Genetic Code Expansion: Recent Developments and Emerging Applications DOI Creative Commons
Yujia Huang, Pan Zhang, Haoyu Wang

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

The concept of genetic code expansion (GCE) has revolutionized the field chemical and synthetic biology, enabling site-specific incorporation noncanonical amino acids (ncAAs) into proteins, thus opening new avenues in research applications across biology medicine. In this review, we cover principles GCE, including optimization aminoacyl-tRNA synthetase (aaRS)/tRNA system advancements translation engineering. Notable developments include refinement aaRS/tRNA pairs, enhancements screening methods, biosynthesis acids. GCE technology span from where it facilitates gene expression regulation protein engineering, to medicine, with promising approaches drug development, vaccine production, editing. review concludes a perspective on future underscoring its potential further expand toolkit Through comprehensive aim provide detailed overview current state technology, challenges, opportunities, frontier represents for novel biological therapeutic applications.

Language: Английский

Design strategies for tetrazine fluorogenic probes for bioorthogonal imaging DOI
Aiwen Yu, Xinyu He, Tianruo Shen

et al.

Chemical Society Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

In this review, recent progress in tetrazine-based fluorogenic probes for bioorthogonal chemistry is comprehensively analyzed, with a focus on advancements probe design and their imaging applications molecular processes live cells.

Language: Английский

Citations

3
Comparative Study of Click Handle Stability in Common Ligation Conditions DOI Creative Commons
Caitlin Fawcett, Justin Watson, Stephen P. Richards

et al.

Bioconjugate Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

Click chemistry efficiently ligates molecular building blocks in a robust and high-yielding manner has found major application the rapid modification of important actors biological systems. However, high reactivity click handles often correlates with decreased stability, which presents significant challenge practical these In current study, we describe survey stability commonly deployed manifolds across range widely used ligation conditions. Incompatible handle condition combinations are identified, kinetic half-lives side products each undesired reaction determined, including assessment over extended periods protein environment. This data set provides researchers roadmap to expediently determine most appropriate conditions for any given bioorthogonal application, thus elevating probability success procedures that utilize chemistry.

Language: Английский

Citations

0
Biorthogonal combined gene editing technology being applied to tumor treatment DOI
Huang Wei

Highlights in Science Engineering and Technology, Journal Year: 2025, Volume and Issue: 139, P. 294 - 300

Published: April 28, 2025

Because of the special complexity tumor treatment, it has been a hot topic in medical research for long time. Although CRISPR system assisted immunotherapy shown to be good option, still lacks defect targeting. The discovery biological orthogonal reaction provides new method attenuating and enhancing efficacy immunotherapy. In this paper, is introduced, application technology gene editing therapy studied. study confirmed that response can used as an assistant enhance mediated targeted reduce side effects, which idea therapy. its high stability well extremely fast rate, newly discovered pentavariant diheterocyclic monosubstituted tetrazine considered combination with protein anchoring, controllable activation T cell epitopes, construction modular platforms or participation regulation base editors future achieve purpose treatment.

Language: Английский

Citations

0
Cost‐effective synthesis of unsymmetric tetrazines DOI Creative Commons
Jangsun Hwang, Bokyung Yoon, Sung‐Eun Suh

et al.

Bulletin of the Korean Chemical Society, Journal Year: 2024, Volume and Issue: 45(10), P. 867 - 872

Published: Oct. 1, 2024

Abstract 1,2,4,5‐Tetrazines serve as versatile two‐carbon synthons, essential for synthesizing a variety of (hetero)aromatic compounds. Their specific reactivity with certain dienophiles, without interfering biochemical processes, makes them ideal bioorthogonal ligation. Despite their broad utility, current synthetic methods are costly and raise safety concerns, particularly during scale‐up. This study introduces safer more cost‐effective strategies utilizing zinc chloride or boron trifluoride diethyl etherate catalysts. These alternatives significantly reduce costs enhance safety, potentially expanding the applicability tetrazine synthesis across broader research domains.

Language: Английский

Citations

0
Computationally Assisted Noncanonical Amino Acid Incorporation DOI Creative Commons

Chengzhu Fang,

Wenyuan Xu,

Chao Liu

et al.

ACS Central Science, Journal Year: 2024, Volume and Issue: 11(1), P. 84 - 90

Published: Dec. 16, 2024

Genetic encoding of noncanonical amino acids (ncAAs) with desired functionalities is an invaluable tool for the study biological processes and development therapeutic drugs. However, existing ncAA incorporation strategies are rather time-consuming have relatively low success rates. Here, we develop a virtual screener based on analysis modeling chemical properties all reported substrates to virtually determine recognition potential candidate ncAAs. Using this screener, designed incorporated several novel Lys Phe derivatives into proteins various downstream applications. Among them, genetic electron-rich analog, 3-dimethylamino-phenylalanine, was successfully applied enhance cation-π interaction between histone methylation its reader proteins. Thus, our provides fast powerful strategy efficiently incorporate ncAAs diverse functionalities.

Language: Английский

Citations

0
Bioorthogonal Cleavage Chemistry: Harnessing the Bond‐Break Reactions for Biomolecule Manipulations in Living Systems DOI Open Access
Yuchao Zhu,

Feng Lin,

Ziqi Liu

et al.

Chinese Journal of Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Comprehensive Summary The advancement of bioorthogonal cleavage reactions (BCRs) has expanded the scope chemistry toolkit, leading to a diverse array innovative biological applications. These include but are not limited precise spatial and temporal activation intracellular probes, prodrugs, proteins, glycans, nucleic acids. Herein, we summarize recent efforts by our group develop BCRs for manipulating functional molecules in living species meet various needs, along with future perspectives this exciting field. How do you get into specific field? Could please share some experiences readers? Chemists good at both forming breaking bonds. Back 2013, while field was continuously thriving, most researchers focused on "ligation" type reactions. Alternatively, I started wonder whether could "bond‐cleavage" reactions? We reviewed literature found that is indeed yet be developed. immediately encouraged my graduate students such began look potential applications new chemistry. soon developed series "cleavage" can triggered metals, small molecules, as well photocatalysis. then applied these activate proteins other biomolecules, allowing gain‐of‐function study their properties inside cells. Small molecule drugs also activated within tumor bed, which led safer more efficient anti‐cancer drugs. Over past decade, have built decaging toolbox generally applicable virtually any interest, persistently working broadening spectrum reaction types This created direction broad utilities life sciences medicine. supervise your students? encourage think independently collaborate widely. would delighted if ideas let me learn something. In addition experimental training, pay great attention cultivating students' logical thinking, English writing, presentation skills. For regular weekly meetings, two will give in‐depth presentations research projects, all members provide constructive discussions suggestions. What important personality scientific research? opinion, traits curiosity, perseverance, critical thinking. favorite journal(s)? ACS Chemical Biology . us advices improving Chinese Journal Chemistry? Consider organizing special issues focus emerging areas chemistry, attract high‐quality submissions increase journal's impact.

Language: Английский

Citations

0
Genetic Code Expansion: Recent Developments and Emerging Applications DOI Creative Commons
Yujia Huang, Pan Zhang, Haoyu Wang

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

The concept of genetic code expansion (GCE) has revolutionized the field chemical and synthetic biology, enabling site-specific incorporation noncanonical amino acids (ncAAs) into proteins, thus opening new avenues in research applications across biology medicine. In this review, we cover principles GCE, including optimization aminoacyl-tRNA synthetase (aaRS)/tRNA system advancements translation engineering. Notable developments include refinement aaRS/tRNA pairs, enhancements screening methods, biosynthesis acids. GCE technology span from where it facilitates gene expression regulation protein engineering, to medicine, with promising approaches drug development, vaccine production, editing. review concludes a perspective on future underscoring its potential further expand toolkit Through comprehensive aim provide detailed overview current state technology, challenges, opportunities, frontier represents for novel biological therapeutic applications.

Language: Английский

Citations

0