His126 Substitution Shifts Perakine Reductase Activity from Carbonyl Reduction to Ene Reduction DOI
Ning Tu, Bin Liu, Zhou Yun

et al.

Organic Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Perakine reductase (PR) is an aldo-keto (AKR) carbonyl reductase. His126 one of the AKR catalytic tetrads in PR. Substitution with any other amino acids except Gln switched PR to ene Molecular simulation suggested that substrate-binding pose and properties 126 residue determined chemoselectivity. Given strict conservation superfamily reductases, modification corresponding site a feasible strategy design AKR-derived novel reductases.

Language: Английский

His126 Substitution Shifts Perakine Reductase Activity from Carbonyl Reduction to Ene Reduction DOI
Ning Tu, Bin Liu, Zhou Yun

et al.

Organic Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Perakine reductase (PR) is an aldo-keto (AKR) carbonyl reductase. His126 one of the AKR catalytic tetrads in PR. Substitution with any other amino acids except Gln switched PR to ene Molecular simulation suggested that substrate-binding pose and properties 126 residue determined chemoselectivity. Given strict conservation superfamily reductases, modification corresponding site a feasible strategy design AKR-derived novel reductases.

Language: Английский

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