Mapping RNA–Protein Interactions via Proximity Labeling‐Based Approaches DOI
Y. Chen, Yuxin Chen, Wei Qin

et al.

Chemistry - An Asian Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract RNA–protein interactions are fundamental to a wide range of biological processes, and understanding these in their native cellular context is both vital challenging. Traditional methods for studying rely on crosslinking, which can introduce artifacts. Recently, proximity labeling‐based techniques have emerged as powerful alternatives, offering crosslinking‐free approach investigate interactions. This review highlights recent advancements the development application labeling methods, focusing RNA‐centric protein‐centric strategies profiling By examining innovative approaches, we aim provide insights into potential enhancing our dynamics various settings.

Language: Английский

Temporal Microenvironment Mapping (microMap) of Intracellular Trafficking Pathways of Cell-Penetrating Peptides Across the Blood-Brain Barrier DOI Creative Commons
Danielle C. Morgan, Steve D. Knutson, Chenmengxiao Pan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Abstract Peptides play critical roles in cellular functions such as signaling and immune regulation, peptide-based biotherapeutics show great promise for treating various diseases. Among these, cell-penetrating peptides (CPPs) are particularly valuable drug delivery due to their ability cross cell membranes. However, the mechanisms underlying CPP-mediated transport, especially across blood-brain barrier (BBB), remain poorly understood. Mapping intracellular CPP pathways is essential advancing systems, neurological disorders, understanding how CPPs navigate complex environment of BBB could enable development more effective brain-targeted therapies. Here, we leverage a nanoscale proximity labeling technique, termed µMap, precisely probe peptide– receptor interactions trafficking photocatalyst-tagged CPPs. The unique advantage μMap platform lies control timing light exposure, which enables collection time-gated data, depending on when blue applied cells. By harnessing this spatiotemporal precision, can uncover key peptide–receptor processes, setting stage new innovations design Figure

Language: Английский

Citations

1
Expanding toolkit for RNP granule transcriptomics DOI Creative Commons
Yuichi Shichino, Shintaro Iwasaki

Polymer Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

Citations

0
Recent advances of photocatalytic biochemical transformations DOI
Surabhi Sinha, Praveen P. Singh,

Sudhanshu Kanaujia

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 157, P. 108320 - 108320

Published: Feb. 26, 2025

Language: Английский

Citations

0
Engineered Proteins and Chemical Tools to Probe the Cell Surface Proteome DOI Creative Commons
Kevin K. Leung, Kaitlin Schaefer, Zhi Lin

et al.

Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

The cell surface proteome, or surfaceome, is the hub for cells to interact and communicate with outside world. Many disease-associated changes are hard-wired within yet approved drugs target less than 50 proteins. In past decade, proteomics community has made significant strides in developing new technologies tailored studying surfaceome all its complexity. this review, we first dive into unique characteristics functions of emphasizing necessity specialized labeling, enrichment, proteomic approaches. An overview surfaceomics methods provided, detailing techniques measure protein expression how leads novel discovery. Next, highlight advances proximity labeling (PLP), showcasing various enzymatic photoaffinity can map protein-protein interactions membrane complexes on surface. We then review role extracellular post-translational modifications, focusing glycosylation, proteolytic remodeling, secretome. Finally, discuss identifying tumor-specific peptide MHC they have shaped therapeutic development. This emerging field neo-protein epitopes constantly evolving, where targets identified at proteome level encompass defined PTMs, complexes, dysregulated cellular tissue locations. Given functional importance biology therapy, view as a critical piece quest neo-epitope

Language: Английский

Citations

0
Mapping RNA–Protein Interactions via Proximity Labeling‐Based Approaches DOI
Y. Chen, Yuxin Chen, Wei Qin

et al.

Chemistry - An Asian Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract RNA–protein interactions are fundamental to a wide range of biological processes, and understanding these in their native cellular context is both vital challenging. Traditional methods for studying rely on crosslinking, which can introduce artifacts. Recently, proximity labeling‐based techniques have emerged as powerful alternatives, offering crosslinking‐free approach investigate interactions. This review highlights recent advancements the development application labeling methods, focusing RNA‐centric protein‐centric strategies profiling By examining innovative approaches, we aim provide insights into potential enhancing our dynamics various settings.

Language: Английский

Citations

0