Intracellular Fate Tracking of DNA Nanostructures† DOI
Yubo Liu, Xia Liu, Linjie Guo

et al.

Chinese Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: June 3, 2025

Comprehensive Summary DNA nanostructures, with their high structural programmability and excellent biocompatibility, have shown tremendous potential in biomedicine applications. This review provides an overview of the self‐assembly principles underlying a particular focus on current techniques for intracellular tracking, highlighting advantages limitations. Building this foundation, behaviors fates nanostructures are discussed, along applications biomedicine. Finally, future research directions proposed, offering insights guidance continued development biomedical fields. Key Scientists In 1982, Seeman pioneered nanotechnology by constructing rigid structure, stabilizing it through disruption sequence symmetry four strands Holliday junctions. [1‐2] breakthrough expanded beyond traditional one‐dimensional double helix, creating two‐dimensional nanoscale polymer material. 2006, Rothemund advanced origami technology, enabling construction various structures. [3] method is now widely employed to assemble diverse shapes dimensions. 2011, Krishnan used fluorescence imaging track dynamic distribution C. elegans , observing spatiotemporal pH changes related endocytosis, thus facilitating use complex biological studies. [4] 2012, Yan developed artificial vaccines precisely organizing antigens adjuvants using TDNs. [5] approach enhanced antigen immune responses while reducing immunogenicity TDNs, providing versatile platform vaccine development. 2013, Liu created enzyme‐triggered permeable hydrogel that could culture, monitor, manipulate single cells, new tool cell communication. [6] 2014, Fan utilized single‐particle tracking total internal reflection microscopy monitor individual TDN structures, revealing clathrin‐dependent uptake microtubule‐dependent transport. [7] same year, Shih, inspired viral encapsulated lipids, improving enzymatic stability immunogenicity. [8] 2018, Ke tracked fate transmission electron microscopy. [9] 2020, Lin TDNs construct multifunctional composite carriers, advancing [10] 2021, Schulman create components employing observe growth application cellular shear force sensing. [11] 2022, et al . constructed large, customizable transmembrane channels surfaces DNA‐origami‐protein nanopores, effective studying macromolecular translocation across membranes. [12] 2024, Castro further elucidated behavior during transport nucleus subsequent diffusion within nucleus. [13]

Language: Английский

Multi-scale delivery dynamics of tetrahedral framework nucleic acids: From organ accumulation to subcellular targeting and precision engineering strategies DOI
Wen Chen, Hao Wang, Qiang Sun

et al.

Chinese Chemical Letters, Journal Year: 2025, Volume and Issue: unknown, P. 111396 - 111396

Published: June 1, 2025

Language: Английский

Citations

0
Intracellular Fate Tracking of DNA Nanostructures† DOI
Yubo Liu, Xia Liu, Linjie Guo

et al.

Chinese Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: June 3, 2025

Comprehensive Summary DNA nanostructures, with their high structural programmability and excellent biocompatibility, have shown tremendous potential in biomedicine applications. This review provides an overview of the self‐assembly principles underlying a particular focus on current techniques for intracellular tracking, highlighting advantages limitations. Building this foundation, behaviors fates nanostructures are discussed, along applications biomedicine. Finally, future research directions proposed, offering insights guidance continued development biomedical fields. Key Scientists In 1982, Seeman pioneered nanotechnology by constructing rigid structure, stabilizing it through disruption sequence symmetry four strands Holliday junctions. [1‐2] breakthrough expanded beyond traditional one‐dimensional double helix, creating two‐dimensional nanoscale polymer material. 2006, Rothemund advanced origami technology, enabling construction various structures. [3] method is now widely employed to assemble diverse shapes dimensions. 2011, Krishnan used fluorescence imaging track dynamic distribution C. elegans , observing spatiotemporal pH changes related endocytosis, thus facilitating use complex biological studies. [4] 2012, Yan developed artificial vaccines precisely organizing antigens adjuvants using TDNs. [5] approach enhanced antigen immune responses while reducing immunogenicity TDNs, providing versatile platform vaccine development. 2013, Liu created enzyme‐triggered permeable hydrogel that could culture, monitor, manipulate single cells, new tool cell communication. [6] 2014, Fan utilized single‐particle tracking total internal reflection microscopy monitor individual TDN structures, revealing clathrin‐dependent uptake microtubule‐dependent transport. [7] same year, Shih, inspired viral encapsulated lipids, improving enzymatic stability immunogenicity. [8] 2018, Ke tracked fate transmission electron microscopy. [9] 2020, Lin TDNs construct multifunctional composite carriers, advancing [10] 2021, Schulman create components employing observe growth application cellular shear force sensing. [11] 2022, et al . constructed large, customizable transmembrane channels surfaces DNA‐origami‐protein nanopores, effective studying macromolecular translocation across membranes. [12] 2024, Castro further elucidated behavior during transport nucleus subsequent diffusion within nucleus. [13]

Language: Английский

Citations

0