Engineered Extracellular Vesicles as a New Class of Nanomedicine DOI Creative Commons

Xiaowei Wen,

Zhengping Hao, Haofan Yin

et al.

Chem & Bio Engineering, Journal Year: 2024, Volume and Issue: 2(1), P. 3 - 22

Published: Oct. 28, 2024

Extracellular vesicles (EVs) are secreted from biological cells and contain many molecules with diagnostic values or therapeutic functions. There has been great interest in academic industrial communities to utilize EVs as tools for diagnosis therapeutics. In addition, can also serve delivery vehicles molecules. An indicator of the enormous is large number review articles published on EVs, focus ranging their biology applications. emerging trend EV research produce "engineered EVs", which essentially enhanced version EVs. engineering be conducted by cell culture condition control, genetic engineering, chemical engineering. Given nanometer-scale sizes potentials, engineered an class nanomedicines. So far, overwhelming majority preclinical studies; there only a very small reported clinical trials. This Review focuses more specific being applications The various approaches producing diseases reviewed. Furthermore, vivo imaging mechanistic understandings, translation aspects discussed. discussion primarily studies while briefly mentioning With continued interdisciplinary efforts biologists, pharmacists, physicians, bioengineers, engineers, could become powerful solution major such neurological, immunological, cardiovascular diseases.

Language: Английский

Al-Based Metal–Organic Nanoslice-Coupled Phospholipid Layer Enables Highly Enriched Biological External Vesicles DOI

Bangming Wang,

Yan Zhao,

Mingzhu Zhao

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Systematic analysis of the information content biological external vesicles (BEVs) is essential for understanding complex relationships between metabolic processes and cells at a systemic level. Although considerable efforts have been made to enrich BEVs, their comprehensive in way that maintains stabilizes maintenance high doses remains challenge. To address this issue, we developed metal–organic nanoslice-coupled exocytosis phospholipid layer method utilized subtle interactions nanoslice molecular membrane achieve convenient efficient enrichment BEVs. The bypassed conventional ultracentrifugation size exclusion separation helped highly preserve internal on By integrating analyzing enriched BEVs elements such as proteins endotoxins, simplicity robustness vesicle surface technology were verified, which provided reliable platform studying cellular events associated with outer expanded applications nanoslice.

Language: Английский

Citations

0
Recent advance on extracellular vesicle labeling: From strategy to probe DOI
Sijung Hu,

Xinwen Chang,

Qiaojiao Ding

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 534, P. 216601 - 216601

Published: March 10, 2025

Language: Английский

Citations

0
Sorting Extrusion-Prepared Coreless Cell Membrane Vesicles of Mixed Orientations by Functional DNA Probes DOI Creative Commons
Jing Ye, Fan Xu, Zhihao Xu

et al.

JACS Au, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Language: Английский

Citations

0
Engineered Extracellular Vesicles as a New Class of Nanomedicine DOI Creative Commons

Xiaowei Wen,

Zhengping Hao, Haofan Yin

et al.

Chem & Bio Engineering, Journal Year: 2024, Volume and Issue: 2(1), P. 3 - 22

Published: Oct. 28, 2024

Extracellular vesicles (EVs) are secreted from biological cells and contain many molecules with diagnostic values or therapeutic functions. There has been great interest in academic industrial communities to utilize EVs as tools for diagnosis therapeutics. In addition, can also serve delivery vehicles molecules. An indicator of the enormous is large number review articles published on EVs, focus ranging their biology applications. emerging trend EV research produce "engineered EVs", which essentially enhanced version EVs. engineering be conducted by cell culture condition control, genetic engineering, chemical engineering. Given nanometer-scale sizes potentials, engineered an class nanomedicines. So far, overwhelming majority preclinical studies; there only a very small reported clinical trials. This Review focuses more specific being applications The various approaches producing diseases reviewed. Furthermore, vivo imaging mechanistic understandings, translation aspects discussed. discussion primarily studies while briefly mentioning With continued interdisciplinary efforts biologists, pharmacists, physicians, bioengineers, engineers, could become powerful solution major such neurological, immunological, cardiovascular diseases.

Language: Английский

Citations

0