Sequencing of d/l-DNA and XNA by Templated-Synthesis DOI Creative Commons
S. V. Joshi,

Patrick Romanens,

Nicolas Winssinger

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Progress in oligonucleotide sequencing has transformed modern biology and medicine. Here we report a fast efficient enzyme-free primer extension of PNA with reversible chain termination its application to DNA XNA sequencing. The approach leverages activated 4-mer PNAs that react templated ligation reaction at μM concentrations within minutes. We demonstrate the fidelity this benefits from reactions performed mixture where every self-complementary 4-mer. can be using whole repertoire 4-mers (256 permutations) parallelized manner. Using combination −1, −2, −3 deletion allows for by MALDI analysis, increment mass each nucleobase assignment. Given nature achiral PNA, further technology used sequence d- or l-DNA as well LNA (XNA).

Language: Английский

Sequencing of d/l-DNA and XNA by Templated-Synthesis DOI Creative Commons
S. V. Joshi,

Patrick Romanens,

Nicolas Winssinger

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Progress in oligonucleotide sequencing has transformed modern biology and medicine. Here we report a fast efficient enzyme-free primer extension of PNA with reversible chain termination its application to DNA XNA sequencing. The approach leverages activated 4-mer PNAs that react templated ligation reaction at μM concentrations within minutes. We demonstrate the fidelity this benefits from reactions performed mixture where every self-complementary 4-mer. can be using whole repertoire 4-mers (256 permutations) parallelized manner. Using combination −1, −2, −3 deletion allows for by MALDI analysis, increment mass each nucleobase assignment. Given nature achiral PNA, further technology used sequence d- or l-DNA as well LNA (XNA).

Language: Английский

Citations

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