Obtaining 20 μm Spatial Resolution with a 2940 nm Laser by IR-MALDESI Mass Spectrometry Imaging DOI
Seth M. Eisenberg, Alena N. Joignant, Kevan T. Knizner

et al.

Journal of the American Society for Mass Spectrometry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

High spatial resolution is a key parameter in mass spectrometry imaging (MSI), enabling greater understanding of system biology and cellular processes. Using novel IR laser with good Gaussian beam quality (

Language: Английский

Lipid imaging mass spectrometry: Towards a new molecular histology DOI Creative Commons

Ibai Cavo,

Olatz Fresnedo, Lorena Mosteiro

et al.

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Journal Year: 2024, Volume and Issue: unknown, P. 159568 - 159568

Published: Oct. 1, 2024

Language: Английский

Citations

2
In Situ molecular profiles of glomerular cells by integrated imaging mass spectrometry and multiplexed immunofluorescence microscopy DOI Creative Commons
Allison B. Esselman, Felipe Moser, Léonore Tideman

et al.

Kidney International, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

2
Deep MALDI-MS Spatial Omics guided by Quantum Cascade Laser Mid-infrared Imaging Microscopy DOI
Lars Gruber, S. Schmidt, Thomas Enzlein

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 14, 2023

Abstract In spatial ‘omics, highly confident molecular identifications are indispensable for the investigation of complex biology and biomarker discovery. However, current mass spectrometry (MS)-based ‘omics must compromise between data acquisition speed biochemical profiling depth, thus often leading to only “putative” identifications. Here, we introduce fast quantum cascade laser mid-infrared imaging microscopy guide MS confined tissue areas high interest, e.g., multicellular spheroid cores or kidney glomeruli, lipidomics at maximized analytical depth utilizing magnetic resonance-MS >10 6 resolution prm-PASEF-MS 2 fragmentation imaging. Instigating selective sulfatide accumulation in arylsulfatase A-deficient mice as ground truth concept, demonstrate that deep QCL-infrared-guided on-tissue unequivocally identifies 120 sulfatides. This approach enables odd-chain sulfatides studies structure-ion mobility-relationships provide chemical rationales improvements ion mobility prediction algorithms. Workflows processing tools provided community resources.

Language: Английский

Citations

4
Correlative Imaging for Comprehensive Molecular Mapping of Individual Cell Types in Biological Tissues DOI
Manxi Yang, Mushfeqa Iqfath, Frederick Nguele Meke

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 4, 2024

Mass spectrometry imaging (MSI) is a powerful technique for label-free spatial mapping of multiple classes biomolecules in tissue sections. However, differences desorption and ionization efficiency different molecules make it challenging to simultaneously map at each omics layer the same sample. Herein, we present correlative method using nanospray electrospray (nano-DESI) MSI, which enables lipids, metabolites, peptides, proteins with cellular-level resolution single section. We demonstrate molecular profiling specific cell types identify truncated peptides mouse pancreatic tissue. Distinct chemical gradients lipids extending from endocrine cells exocrine indicate their roles endocrine-exocrine crosstalk intracellular signaling. The results underscore power developed approach multi-omics analysis that provides deep insights into cellular diversity intricate interactions occur within heterogenous biological tissues.

Language: Английский

Citations

1
Atmospheric Pressure Mass Spectrometry Imaging Using Electrospray-Assisted Laser Desorption/Ionization with Gas Transportation through a Heated Tube and Minimal Sample Preparation DOI Creative Commons

Riku Hirotani,

Yuto Miyoshi,

Varun Sendilraj

et al.

Mass Spectrometry, Journal Year: 2024, Volume and Issue: 13(1), P. A0167 - A0167

Published: Dec. 18, 2024

Mass spectrometry (MS) is a valuable tool that enables label-free analysis and the ability to measure multiple molecules. The atmospheric pressure MS imaging (MSI) method usually requires tedious sample preparation. A simple ionization with minimal preparation needed for high-throughput analysis. We have developed an ion source does not require such as thinning, curing, planarization, or addition of matrix by electrospray-assisted laser desorption/ionization gas transportation (ELDI-GT). transported nitrogen through heated tube electrospray. signal protonated caffeine was measured under different transport conditions. intensity found increase 11-fold changing flow rate temperature from 2.8 cm

Language: Английский

Citations

1
A Lipid Atlas of the Human Kidney DOI Creative Commons
Melissa A. Farrow, Léonore Tideman, Elizabeth K. Neumann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: April 7, 2022

ABSTRACT Tissue atlases provide foundational knowledge on the cellular organization and molecular distributions across classes spatial scales. Here, we construct a comprehensive spatio-molecular lipid atlas of human kidney from 29 donor tissues using integrated multimodal imaging. Our approach leverages high resolution matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) for untargeted mapping, stained microscopy histopathological assessment, tissue segmentation autofluorescence microscopy. With combination unsupervised, supervised, interpretive machine learning, provides multivariate profiles specific multicellular functional units (FTUs) nephron, including glomerulus, proximal tubules, thick ascending limb, distal collecting ducts. In total, consists tens thousands FTUs millions measurements. Detailed patient, clinical, histopathologic information allowed data to be mined based these features. As examples, highlight discovery how are altered with sex differences in body index.

Language: Английский

Citations

4
Analysis of Urine-Formed Elements: Overview DOI
Lei Zheng, Mianyang Li, Haixia Li

et al.

Published: Jan. 1, 2024

The urinary system consists of the kidneys, ureters, bladder, urethra (Fig. 1.1). Its main function is to expel waste and excess water produced during body's metabolic process, maintaining balance stability internal environment. kidneys generate urine, which transported through ureters bladder for storage then expelled from body [2].

Language: Английский

Citations

0
The histomorphology of the senescent kidney – the clinical relevance of specimen and biopsy findings in the elderly native kidneys DOI
Nicolas Kozakowski

Current Opinion in Urology, Journal Year: 2024, Volume and Issue: 34(3), P. 170 - 175

Published: Feb. 26, 2024

Renal pathology is crucial in diagnosing the ageing kidney. Recent technological advances enabled high-resolution molecular investigations into complex mechanisms of and senescence.

Language: Английский

Citations

0
Multidimensional mass profiles increase confidence in bacterial identification when using low-resolution mass spectrometers DOI Creative Commons

Zachary J. Sasiene,

Erick S. LeBrun, Nileena Velappan

et al.

The Analyst, Journal Year: 2024, Volume and Issue: 149(13), P. 3564 - 3574

Published: Jan. 1, 2024

Field-forward analytical technologies, such as portable mass spectrometry (MS), enable essential capabilities for real-time monitoring and point-of-care diagnostic applications. Significant recent investments improving the features of miniaturized spectrometers various new applications outside small molecule detection. Most notably, addition tandem scans (MS/MS) allows instrument to isolate fragment ions increase specificity by measuring unique chemical signatures interest. Notwithstanding these technological advancements, low-cost, systems still struggle confidently identify clinically significant organisms interest, bacteria, viruses, proteinaceous toxins, due limitations in resolving power. To overcome limitations, we developed a novel multidimensional fingerprinting technique that uses low-resolution spectral profiles. We demonstrated method's differentiating four different including attentuated strains

Language: Английский

Citations

0
B cell expression of the enzyme PexRAP, an intermediary in ether lipid biosynthesis, promotes antibody responses and germinal center size DOI
Sung Hoon Cho, Marissa A. Jones,

Kaylor Meyer

et al.

Published: Oct. 18, 2024

The qualities of antibody (Ab) responses provided by B lymphocytes and their plasma cell (PC) descendants are crucial facets to vaccines microbes. Metabolic processes products regulate aspects proliferation differentiation into germinal center (GC) PC states as well Ab diversification. However, there is little information about lymphoid cell-intrinsic functions enzymes that mediate ether lipid biosynthesis, including a major class membrane phospholipids. Imaging mass spectrometry (IMS) results had indicated concentrations number these phospholipids were substantially enhanced in GC compared the background average spleens. it was not clear if biosynthesis cells basis for this finding, or whether such contributes physiology responses. Ether can involve enzyme PexRAP, product

Language: Английский

Citations

0