A Streamlined Workflow for Microscopy-Driven MALDI Imaging Mass Spectrometry Data Collection

Journal of the American Society for Mass Spectrometry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a rapidly advancing technology for biomedical research. As spatial resolution increases, however, so do acquisition time, file size, and experimental cost, which increases the need to perform precise sampling of targeted tissue regions optimize biological information gleaned from an experiment minimize wasted resources. The ability define instrument measurement based on key features automatically sample these specific interest (ROIs) addresses this challenge. Herein, we demonstrate workflow using standard software that allows direct microscopy-defined by MALDI IMS. Three case studies are included, highlighting different methods defining common types─manual annotation vasculature in human brain tissue, automated segmentation renal functional units across whole slide images custom algorithms, dispersed HeLa cells open-source software. Each minimizes data unnecessary dramatically throughput while uncovering molecular heterogeneity within ROIs. This provides approachable method spatially IMS driven microscopy as part multimodal studies.

Language: Английский

Obtaining 20 μm Spatial Resolution with a 2940 nm Laser by IR-MALDESI Mass Spectrometry Imaging

Journal of the American Society for Mass Spectrometry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

High spatial resolution is a key parameter in mass spectrometry imaging (MSI), enabling greater understanding of system biology and cellular processes. Using novel IR laser with good Gaussian beam quality (

Language: Английский