Mutations in the Staphylococcus aureus Global Regulator CodY confer tolerance to an interspecies redox-active antimicrobial DOI Creative Commons
Anthony M. Martini,

Sara A. Alexander,

Anupama Khare

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(3), P. e1011610 - e1011610

Published: March 7, 2025

Bacteria often exist in multispecies communities where interactions among different species can modify individual fitness and behavior. Although many competitive have been described, molecular adaptations that counter this antagonism preserve or increase remain underexplored. Here, we characterize the adaptation of Staphylococcus aureus to pyocyanin, a redox-active interspecies antimicrobial produced by Pseudomonas aeruginosa , co-infecting pathogen frequently isolated from wound chronic lung infections with S. . Using experimental evolution, identified mutations conserved global transcriptional regulator, CodY, confer tolerance pyocyanin thereby enhance survival A tolerant CodY mutant also had advantage co-culture P. likely through specifically pyocyanin. The response indicated two-pronged defensive compared wild type. First, strongly suppressed metabolism, downregulating pathways associated core especially translation-associated genes, upon exposure Metabolic suppression via ATP depletion was sufficient provide comparable protection against wild-type strain. Second, while both strains upregulated oxidative stress exposure, overexpressed multiple genes We determined catalase overexpression critical as its absence eliminated impart strain purified WT Together, these results suggest responses reduced metabolism an increased contribute mutant. Our data thus new mechanistic insight into toward interbacterial altered regulation facilitates multifaceted protective cellular responses.

Language: Английский

Mutations in the Staphylococcus aureus Global Regulator CodY confer tolerance to an interspecies redox-active antimicrobial DOI Creative Commons
Anthony M. Martini,

Sara A. Alexander,

Anupama Khare

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(3), P. e1011610 - e1011610

Published: March 7, 2025

Bacteria often exist in multispecies communities where interactions among different species can modify individual fitness and behavior. Although many competitive have been described, molecular adaptations that counter this antagonism preserve or increase remain underexplored. Here, we characterize the adaptation of Staphylococcus aureus to pyocyanin, a redox-active interspecies antimicrobial produced by Pseudomonas aeruginosa , co-infecting pathogen frequently isolated from wound chronic lung infections with S. . Using experimental evolution, identified mutations conserved global transcriptional regulator, CodY, confer tolerance pyocyanin thereby enhance survival A tolerant CodY mutant also had advantage co-culture P. likely through specifically pyocyanin. The response indicated two-pronged defensive compared wild type. First, strongly suppressed metabolism, downregulating pathways associated core especially translation-associated genes, upon exposure Metabolic suppression via ATP depletion was sufficient provide comparable protection against wild-type strain. Second, while both strains upregulated oxidative stress exposure, overexpressed multiple genes We determined catalase overexpression critical as its absence eliminated impart strain purified WT Together, these results suggest responses reduced metabolism an increased contribute mutant. Our data thus new mechanistic insight into toward interbacterial altered regulation facilitates multifaceted protective cellular responses.

Language: Английский

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