Resolving spatiotemporal dynamics in bacterial multicellular populations: approaches and challenges

Microbiology and Molecular Biology Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

SUMMARY The development of multicellularity represents a key evolutionary transition that is crucial for the emergence complex life forms. Although has traditionally been studied in eukaryotes, it originates prokaryotes. Coordinated aggregation individual cells within confines colony results emerging, higher-level functions benefit population as whole. During differentiation, an almost infinite number ecological and physiological population-forming forces are at work, creating complex, intricate structures with divergent functions. Understanding assembly dynamics such populations requires resolving or cell groups macroscopic structures. Addressing how each contributes to collective action pushing resolution boundaries technologies will be presented this review. In particular, single-cell techniques provide powerful tools studying bacterial unprecedented spatial temporal resolution. These advancements include novel microscopic techniques, mass spectrometry imaging, flow cytometry, transcriptomics, single-bacteria RNA sequencing, integration spatiotemporal transcriptomics microscopy, alongside advanced microfluidic cultivation systems. This review encourages exploring synergistic potential new study multicellularity, particular focus on individuals differentiated biofilms (colonies). It highlights level understanding their respective can elucidate overarching multicellular populations.

Language: Английский

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Trends in Mass Spectrometry-Based Single-Cell Proteomics

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

InfoMetrics Analytical ChemistryASAPArticle CiteCitationCitation and abstractCitation referencesMore citation options ShareShare onFacebookXWeChatLinkedInRedditEmailBlueskyJump toExpandCollapse ReviewMarch 16, 2025Trends in Mass Spectrometry-Based Single-Cell ProteomicsClick to copy article linkArticle link copied!Ximena Sanchez-AvilaXimena Sanchez-AvilaDepartment of Chemistry Biochemistry, Brigham Young University, Provo, Utah 84602, United StatesMore by Ximena Sanchez-AvilaView BiographyRaphaela M. de OliveiraRaphaela OliveiraDepartment Raphaela OliveiraView BiographySiqi HuangSiqi HuangDepartment Siqi HuangView BiographyChao WangChao WangDepartment Chao WangView Biographyhttps://orcid.org/0009-0008-6197-2985Ryan T. Kelly*Ryan KellyDepartment States*Email: [email protected]More Ryan KellyView Biographyhttps://orcid.org/0000-0002-3339-4443Other Access OptionsAnalytical ChemistryCite this: Anal. Chem. 2025, XXXX, XXX, XXX-XXXClick citationCitation copied!https://pubs.acs.org/doi/10.1021/acs.analchem.5c00661https://doi.org/10.1021/acs.analchem.5c00661Published March 2025 Publication History Received 28 January 2025Accepted February 2025Revised 23 2025Published online 16 2025review-article© American Chemical SocietyRequest reuse permissionsACS Publications© SocietySubjectswhat are subjects Article automatically applied from the ACS Subject Taxonomy describe scientific concepts themes article. Cells Isolation spectrometry Peptides proteins Sample preparation Note: In lieu an abstract, this is article's first page. Read To access article, please review available below. Get instant Purchase for 48 hours. Check out below using your ID or as a guest. Restore my guest Recommended through Your Institution You may have institution. institution does not content. Add change let them know you'd like include access. Through Recommend Name Loading Institutional Login Options... Change Explore subscriptions institutions Log with if you previously purchased it member benefits. hours $48.00 cart Checkout Cited By Click section linkSection copied!This has yet been cited other publications.Download PDF e-AlertsGet e-AlertsAnalytical copied!https://doi.org/10.1021/acs.analchem.5c00661Published 2025© permissionsArticle Views6Altmetric-Citations-Learn about these metrics closeArticle Views COUNTER-compliant sum full text downloads since November 2008 (both HTML) across all individuals. These regularly updated reflect usage leading up last few days.Citations number articles citing calculated Crossref daily. Find more information counts.The Altmetric Attention Score quantitative measure attention that research received online. Clicking on donut icon will load page at altmetric.com additional details score social media presence given how calculated.Recommended Articles

Language: Английский

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Immunopeptidomics in the Era of Single-Cell Proteomics

Biology, Journal Year: 2023, Volume and Issue: 12(12), P. 1514 - 1514

Published: Dec. 12, 2023

Immunopeptidomics, as the analysis of antigen peptides being presented to immune system via major histocompatibility complexes (MHC), is seen an imperative tool for identifying epitopes vaccine development treat cancer and viral bacterial infections well parasites. The field has made tremendous strides over last 25 years but currently still faces challenges in sensitivity throughput widespread applications personalized medicine large studies. Cutting-edge technological advancements sample preparation, liquid chromatography mass spectrometry, data analysis, however, are transforming field. This perspective showcases how advent single-cell proteomics accelerated this transformation immunopeptidomics recent will pave way even more sensitive higher-throughput analyses.

Language: Английский

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Proteomics and Its Application to the Human Pathogenic Fungus Aspergillus fumigatus

Published: Jan. 1, 2024

Language: Английский

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