Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1334, P. 343244 - 343244
Published: Sept. 14, 2024
Language: Английский
Acta Physiologica, Journal Year: 2025, Volume and Issue: 241(5)
Published: April 4, 2025
Leucine-rich repeat transmembrane proteins (LRRTMs) are synaptic adhesion that regulate synapse development and function. They interact transsynaptically with presynaptic binding partners to promote differentiation. Polymorphisms of LRRTM4, one the four members this protein family, have been linked multiple neuropsychiatric disorders childhood aggression, but underlying mechanisms physiological function LRRTM4 during behavior currently unclear. To characterize role gene for brain function, we combined a battery behavioral assays transcriptomic metabolomic analyses, using zebrafish as model system. Our findings revealed lrrtm4l1, brain-specific orthologue human exhibits region-specific expression pattern similar humans, strong in dorsal telencephalon, area critical regulating emotional-affective social behavior. lrrtm4l1-/- displayed heightened anxiety reduced while locomotion remained unaffected by knockout. Transcriptomic analysis telencephalon identified over 100 differentially expressed genes between wild-type mutant an enrichment pathways related plasticity neuronal signaling. The metabolome showed alterations, particularly dopaminergic adenosinergic neurotransmitter systems. These suggest LRRTMs may functions beyond their established excitatory development, such regulation neurotransmission Targeting therapeutically thus be interesting novel approach alleviate excessive aggression or associated number conditions.
Language: Английский
Citations
1
Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113448 - 113448
Published: March 1, 2025
Language: Английский
Citations
0
TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118244 - 118244
Published: March 1, 2025
Language: Английский
Citations
0
Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(18)
Published: Sept. 1, 2024
ABSTRACT Abnormal levels of catecholamine (CA) neurotransmitters and their metabolites in biological fluids can lead to various neurological disorders. Herein, a boric acid‐functionalized hypercrosslinked polymer was prepared utilized as sorbent for the dispersive solid‐phase extraction CAs rat serum. By combination with high‐performance liquid chromatography‐fluorescence detector, parameters seven target analytes were optimized. Under optimal condition, methodology quantitative analysis serum samples established. The limits detection quantification found be ranges 0.010–0.015 0.033–0.050 ng/mL, respectively. results demonstrated satisfactory recoveries, values ranging from 88.02% 113.27%, accompanied by relative standard deviations within range 2.69%–9.59%. In addition, method showed good anti‐interference ability (matrix effect ranged 2.64% 18.07%). developed validated determination normal Alzheimer's disease model rats’ serum, which proved promising application neurotransmitter samples.
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 77 - 77
Published: Dec. 25, 2024
Oxysterols, as metabolites of cholesterol, play a key role in cholesterol homeostasis, autophagosome formation, and regulation immune responses. Disorders oxysterol metabolism are closely related to the pathogenesis neurodegenerative diseases. To systematically investigate profound molecular regulatory mechanisms diseases, it is necessary quantify oxysterols their central peripheral biospecimens simultaneously accurately. However, there lot unsolved problems with existing methods, such hindrance applying single method different biological specimens or challenge simultaneous quantification due differential groups on ends side chains. Herein, according physicochemical properties structure oxysterols, an optimized liquid chromatography-tandem mass spectrometry for was established by optimizing sample preparation process, chromatographic conditions, mobile phase pH, solvent selection. Seven were detected this method, including 27-hydroxycholesterol, 7α-hydroxycholesterol, 7α,27-dihydroxycholesterol, 7-dehydrocholesterol, 7α-hydroxy-3-oxo-4-cholestenoic acid, 3-hydroxy-5-cholestenoic 24(S)-hydroxycholesterol. Non-derivatization extraction methyl tert-butyl ether used biospecimens, followed separation phenyl hexyl column. By repeated validation, exhibited satisfactory linearity, precision, recovery, sensitivity, repeatability, stability, successfully applied detection plasma, cerebral cortex, liver mouse. In summary, our enables concurrent analysis various presenting broad range applicability.
Language: Английский
Citations
1
Data in Brief, Journal Year: 2024, Volume and Issue: 55, P. 110632 - 110632
Published: June 19, 2024
The dataset contains the results of a cross-sectional analysis conducted in Telangana, India, to investigate effects pesticide exposure agricultural regions. It includes 341 exposed individuals and 152 controls three districts including 15 farming villages. Blood urine samples were analysed determine various concentrations, six dialkyl phosphate (DAP) metabolites, dimethyl (DMP), diethyl (DEP), thiophosphate (DMTP), dimethyldithiophosphate (DMDTP), diethylthiophosphate (DETP) diethyldithiophosphate (DEDTP), as well enzyme activity acetylcholinesterase (AChE) measured using advanced analytical methods. data provide information on profiles, biomarkers relationship between duration AChE activity. These emphasise importance addressing pesticide-related health problems argue for proactive measures mitigate harmful agriculture.
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0
Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1334, P. 343244 - 343244
Published: Sept. 14, 2024
Language: Английский
Citations
0