
Macromolecular Rapid Communications, Journal Year: 2025, Volume and Issue: unknown
Published: April 28, 2025
Abstract A simple method is presented for preparing polypeptide nanoparticles using hydrophilic biosynthetic ε‐poly(lysine) (εPL) as a reactive surfactant under dispersion polymerization conditions. In situ graft of benzyl‐L‐glutamic acid N ‐carboxyanhydride (BLG‐NCA) triggers the self‐assembly amphiphilic copolymers into nanoparticles, which are colloidally stabilized by remaining εPL amino groups at particle surface. The average nanoparticle diameter can be controlled in range 40–120 nm varying initiator‐to‐NCA ratio, demonstrated correlation between copolymer molecular weight (measured size exclusion chromatography) and z‐average dynamic light scattering). Secondary structure analysis indicates that α‐helical conformation poly(benzyl‐L‐glutamate) (PBLG) grafts plays role both accelerating NCA stabilizing nanostructures. This approach readily scalable to high concentrations offers straightforward route peptidomimetic entirely composed acids, with promising potential nanomedicine applications.
Language: Английский