Molecular Architecture of the Mouse Nervous System

Cell, Journal Year: 2018, Volume and Issue: 174(4), P. 999 - 1014.e22

Published: Aug. 1, 2018

The mammalian nervous system executes complex behaviors controlled by specialized, precisely positioned, and interacting cell types. Here, we used RNA sequencing of half a million single cells to create detailed census types in the mouse system. We mapped spatially derived hierarchical, data-driven taxonomy. Neurons were most diverse grouped developmental anatomical units expression neurotransmitters neuropeptides. Neuronal diversity was driven genes encoding identity, synaptic connectivity, neurotransmission, membrane conductance. discovered seven distinct, regionally restricted astrocyte that obeyed boundaries correlated with spatial distribution key glutamate glycine neurotransmitters. In contrast, oligodendrocytes showed loss regional identity followed secondary diversification. resource presented here lays solid foundation for understanding molecular architecture enables genetic manipulation specific

Language: Английский

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Neuronal cell-type classification: challenges, opportunities and the path forward

Nature reviews. Neuroscience, Journal Year: 2017, Volume and Issue: 18(9), P. 530 - 546

Published: Aug. 3, 2017

Language: Английский

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TFOS DEWS II pain and sensation report

The Ocular Surface, Journal Year: 2017, Volume and Issue: 15(3), P. 404 - 437

Published: July 1, 2017

Language: Английский

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Neural circuits for pain: Recent advances and current views

Science, Journal Year: 2016, Volume and Issue: 354(6312), P. 578 - 584

Published: Nov. 3, 2016

The mammalian nervous system encodes many different forms of pain, from those that arise as a result short-term low-grade interactions with noxious thermal, chemical, or mechanical sources to more serious pain induced by trauma and disease. In this Review, we highlight recent advances in our understanding the neural circuits encode these types pain. Promising therapeutic strategies based on are also highlighted.

Language: Английский

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Transcriptional Reprogramming of Distinct Peripheral Sensory Neuron Subtypes after Axonal Injury

Neuron, Journal Year: 2020, Volume and Issue: 108(1), P. 128 - 144.e9

Published: Aug. 17, 2020

Language: Английский

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Neuronal atlas of the dorsal horn defines its architecture and links sensory input to transcriptional cell types

Nature Neuroscience, Journal Year: 2018, Volume and Issue: 21(6), P. 869 - 880

Published: April 20, 2018

Language: Английский

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The emergence of transcriptional identity in somatosensory neurons

Nature, Journal Year: 2020, Volume and Issue: 577(7790), P. 392 - 398

Published: Jan. 8, 2020

Language: Английский

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Detecting Activated Cell Populations Using Single-Cell RNA-Seq

Neuron, Journal Year: 2017, Volume and Issue: 96(2), P. 313 - 329.e6

Published: Oct. 1, 2017

Language: Английский

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An Atlas of Vagal Sensory Neurons and Their Molecular Specialization

Cell Reports, Journal Year: 2019, Volume and Issue: 27(8), P. 2508 - 2523.e4

Published: May 1, 2019

Sensory functions of the vagus nerve are critical for conscious perceptions and monitoring visceral in cardio-pulmonary gastrointestinal systems. Here, we present a comprehensive identification, classification, validation neuron types neural crest (jugular) placode (nodose) derived vagal ganglia by single-cell RNA sequencing (scRNA-seq) transcriptomic analysis. Our results reveal major differences between neurons from different embryonic origins. Jugular exhibit fundamental similarities to somatosensory spinal neurons, including types, such as C-low threshold mechanoreceptors (C-LTMRs), A-LTMRs, Aδ-nociceptors, cold-, mechano-heat C-nociceptors. In contrast, nodose ganglion contains 18 distinct dedicated surveying physiological state internal body. vast diversity many previously unanticipated well proposed that consistent with chemoreceptors, nutrient detectors, baroreceptors, stretch volume respiratory, gastrointestinal, cardiovascular

Language: Английский

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Massively Parallel Single Nucleus Transcriptional Profiling Defines Spinal Cord Neurons and Their Activity during Behavior

Cell Reports, Journal Year: 2018, Volume and Issue: 22(8), P. 2216 - 2225

Published: Feb. 1, 2018

To understand the cellular basis of behavior, it is necessary to know cell types that exist in nervous system and their contributions function. Spinal networks are essential for sensory processing motor behavior provide a powerful identifying correlates behavior. Here, we used massively parallel single nucleus RNA sequencing (snRNA-seq) create an atlas adult mouse lumbar spinal cord. We identified molecularly characterized 43 neuronal populations. Next, leveraged snRNA-seq approach unbiased identification populations were active following using transcriptional signature activity. This can be future link gene expression data with dynamic biological responses injury, disease.

Language: Английский

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