A Walk on the Wild Side: Genome Editing of Tuber-Bearing Solanum bulbocastanum

Plants, Journal Year: 2024, Volume and Issue: 13(7), P. 1044 - 1044

Published: April 8, 2024

Solanum bulbocastanum is a wild diploid tuber-bearing plant. We here demonstrate transgene-free genome editing of S. protoplasts and regeneration gene-edited plants. use ribonucleoproteins, consisting Cas9 sgRNA, assembled in vitro, to target gene belonging the nitrate peptide transporter family. Four different sgRNAs were designed we observed efficiency gene-editing protoplast pool between 8.5% 12.4%. Twenty-one plants re-generated from microcalli developed individual protoplasts. In three found that had been edited. Two edited deletion mutations introduced into both alleles, whereas one only mutation alleles. Our work demonstrates protocols for transformation tuberosum can be optimized applied species.

Language: Английский

CRISPR/Cas Mediated Genome Engineering in Plants: Application and Future Prospective

Published: April 5, 2024

Genetic engineering in the field for designing crop improvement has become an essential element responding to increasing need food safety, environmental sustainability, and climate-resilient crops. The latest molecular technology is used by many breeders today achieve remarkable results. Fast genome editing currently being seen a variety of organisms, including plants, because high specificity, effectiveness, low production cost CRISPR/Cas [Clustered regulatory interspaced short palindromic repeat (CRISPR)-associated protein (Cas)] technology. Its applications developing higher-yielding, nutrition rich, disease resistance, stress-tolerant crops fruits, vegetables. Therefore, this potential revolutionize agriculture contribute global security. Cas provides enzymatic machinery necessary acquisition new cells that target invasion elements. proteins such as Cas9, Cas12, Cas13, Cas14, among others, have distinct architectures been create genetic tools improve features are important agriculture. versatility accelerated genomic analysis facilitated use manipulate alter nucleic acid sequences different organisms. This review evolution CRISPR exploring its mechanisms contrasting it with traditional breeding transgenic approaches stress tolerance. We will also discuss system explore workings three known exist: Cas14. edited do not foreign DNA inserted into their genome, therefore, they considered non-transgenic bodies most countries.

Language: Английский

Strategies and Protocols for Optimized Genome Editing in Potato

The CRISPR Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

The potato family includes a highly diverse cultivar repertoire and has high potential for nutritional yield improvement refinement but must in line with other crops be adapted to biotic abiotic stresses, example, accelerated by climate change environmental demands. combination of pluripotency, ploidy, relative ease protoplast isolation, transformation, regeneration together clonal propagation through tubers makes suitable precise genetic engineering. Most varieties are tetraploid having very prevalence length polymorphisms small nucleotide between alleles, often complicating CRISPR-Cas editing designs strategies. can divided into (i) characterization target area

Language: Английский