DNA-binding proteins from MBD through ZF to BEN: recognition of cytosine methylation status by one arginine with two conformations DOI Creative Commons

Xing Zhang,

Robert Blumenthal, Xiaodong Cheng

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Abstract Maintenance methylation, of palindromic CpG dinucleotides at DNA replication forks, is crucial for the faithful mitotic inheritance genomic 5-methylcytosine (5mC) methylation patterns. MBD proteins use two arginine residues to recognize symmetrically-positioned methyl groups in fully-methylated 5mCpG/5mCpG and 5mCpA/TpG dinucleotides. In contrast, C2H2 zinc finger (ZF) CpA, whether methylated or not, within longer specific sequences a site- strand-specific manner. Unmethylated sites, often island (CGI) promoters, need protection by protein factors maintain their hypomethylated status. Members BEN domain bind CGCG CACG elements CGIs regulate gene expression. Despite overall structural diversity, MBD, ZF all guanine, adopting either ‘straight-on’ ‘oblique’ conformation. The straight-on conformation accommodates group (5mC/T)pG dinucleotide, while oblique can clash with 5mC, leading preferential binding unmethylated sequences.

Language: Английский

Overcoming gene dosage barriers in mammalian development: An imprinting balancing act DOI
Zhengfeng Liu, Marisa S. Bartolomei

Cell stem cell, Journal Year: 2025, Volume and Issue: 32(3), P. 333 - 335

Published: March 1, 2025

Language: Английский

Citations

0
DNA-binding proteins from MBD through ZF to BEN: recognition of cytosine methylation status by one arginine with two conformations DOI Creative Commons

Xing Zhang,

Robert Blumenthal, Xiaodong Cheng

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Abstract Maintenance methylation, of palindromic CpG dinucleotides at DNA replication forks, is crucial for the faithful mitotic inheritance genomic 5-methylcytosine (5mC) methylation patterns. MBD proteins use two arginine residues to recognize symmetrically-positioned methyl groups in fully-methylated 5mCpG/5mCpG and 5mCpA/TpG dinucleotides. In contrast, C2H2 zinc finger (ZF) CpA, whether methylated or not, within longer specific sequences a site- strand-specific manner. Unmethylated sites, often island (CGI) promoters, need protection by protein factors maintain their hypomethylated status. Members BEN domain bind CGCG CACG elements CGIs regulate gene expression. Despite overall structural diversity, MBD, ZF all guanine, adopting either ‘straight-on’ ‘oblique’ conformation. The straight-on conformation accommodates group (5mC/T)pG dinucleotide, while oblique can clash with 5mC, leading preferential binding unmethylated sequences.

Language: Английский

Citations

2