Ultrastructure expansion microscopy: Enlarging our perspective on apicomplexan cell division

Journal of Microscopy, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Abstract Apicomplexans, a large phylum of protozoan intracellular parasites, well known for their ability to invade and proliferate within host cells, cause diseases with major health economic impacts worldwide. These parasites are responsible conditions such as malaria, cryptosporidiosis, toxoplasmosis, which affect humans other animals. Apicomplexans exhibit complex life cycles, marked by diverse modes cell division, closely associated pathogenesis. All the unique structural evolutionary characteristics apicomplexan biology underlying stage transitions, singular mechanisms division alongside biomedical relevance have captured attention parasitologists all times. Traditional light electron microscopy set fundamental foundations our understanding these including distinction among division. This has been more recently complemented advances through implementation superresolution fluorescence microscopy, variants cryo‐EM tomography, revealing intricate details organelles Ultrastructure Expansion Microscopy emerged transformative, accessible approach that enhances resolution physically expanding samples isometrically, allowing nanoscale visualisation on standard microscopes. In this work, we review most recent contributions U‐ExM its improvements innovations, in providing unprecedented insights into ultrastructure mechanisms, focusing particularly We highlight power combination protein‐specific labelling, aiding long oversighted detailed assembly parasite‐specific structures, conoid Plasmodia , apical‐basal axis Toxoplasma respectively, during new parasite assembly. Altogether, reveal conserved features across species while nearing super resolution. The development methodologies different technologies crucial advancing mechanistic biology, offering perspectives may facilitate novel therapeutic strategies against apicomplexan‐caused diseases.

Language: Английский

Omics Biology in Diagnosis of Diseases: Towards Empowering Genomic Medicine from an Evolutionary Perspective

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1637 - 1637

Published: Dec. 10, 2024

In this section, we reintroduce the original aims and scope of Special Issue entitled “Omics Biology in Diagnosis Diseases: Advances Bioinformatics Data Analyses”, enabling readers to find an appropriate framing for remainder present closing editorial [...]

Language: Английский