Three-Dimensional Cultured Human Nasal Epithelial Cell Model for Testing Respiratory Toxicity and Neurotoxicity of Air Pollutants DOI
Huan Wang, Ting Xu,

Jing Han

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Accumulating evidence suggests a strong correlation between air pollution and neurological disorders; however, appropriate models methodologies are currently lacking. In this study, human nasal RPMI 2650 cell model based on air-liquid interface culture was discovered to possess olfactory epithelial cells. Two short-chain per- polyfluoroalkyl substances (PFAS), PFBA PFHxA, were used validate the performance of model. RNA sequencing initially revealed adverse effects two PFAS at environmentally relevant concentrations. Their key functions, including barrier protection, solute transport, neuronal activity, separately investigated. Both PFHxA disrupted membrane integrity increased cellular transport capacity, as indicated by upregulation ABC transporters. Additionally, they inhibited tight junction proteins, ZO-1, claudin-3, occludin, while increasing mucin expression mucus secretion. exhibited stronger in most assays uniquely induced significant NOTCH1 (p < 0.05), highlighting its potential hazards neurons. This study proposed novel vitro test with matched respiratory end points, which expected improve toxicological research risk assessment pollutants.

Language: Английский

Three-Dimensional Cultured Human Nasal Epithelial Cell Model for Testing Respiratory Toxicity and Neurotoxicity of Air Pollutants DOI
Huan Wang, Ting Xu,

Jing Han

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Accumulating evidence suggests a strong correlation between air pollution and neurological disorders; however, appropriate models methodologies are currently lacking. In this study, human nasal RPMI 2650 cell model based on air-liquid interface culture was discovered to possess olfactory epithelial cells. Two short-chain per- polyfluoroalkyl substances (PFAS), PFBA PFHxA, were used validate the performance of model. RNA sequencing initially revealed adverse effects two PFAS at environmentally relevant concentrations. Their key functions, including barrier protection, solute transport, neuronal activity, separately investigated. Both PFHxA disrupted membrane integrity increased cellular transport capacity, as indicated by upregulation ABC transporters. Additionally, they inhibited tight junction proteins, ZO-1, claudin-3, occludin, while increasing mucin expression mucus secretion. exhibited stronger in most assays uniquely induced significant NOTCH1 (p < 0.05), highlighting its potential hazards neurons. This study proposed novel vitro test with matched respiratory end points, which expected improve toxicological research risk assessment pollutants.

Language: Английский

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