Metagenomic analysis reveals distinct patterns of denitrification gene abundance across soil moisture, nitrate gradients DOI
Sarah Nadeau, Constance A. Roco,

Spencer J. Debenport

et al.

Environmental Microbiology, Journal Year: 2019, Volume and Issue: 21(4), P. 1255 - 1266

Published: March 9, 2019

Summary This study coupled a landscape‐scale metagenomic survey of denitrification gene abundance in soils with situ measurements to show how environmental factors shape distinct communities that exhibit varying activity. Across hydrologic gradient, the distribution total genes ( nap/nar + nirK/nirS cNor/qNor nosZ) inferred from read exhibited no consistent patterns. However, when were considered independently, nirS , cNor and nosZ was positively associated areas higher soil moisture, nitrate annual rates, whereas nirK qNor negatively these factors. These results suggest conditions, particular moisture nitrate, select for are characterized by differential encoding apparently functionally redundant proteins. In contrast, taxonomic analysis did not identify notable variability denitrifying community composition across sites. While capacity denitrify ubiquitous sites, energetic costs, such as appear confer selective advantage microbial experiencing more frequent saturation greater inputs. suggests metagenomics can help hotspots could be protected or enhanced treat non‐point source nitrogen pollution.

Language: Английский

Recent advances in partial denitrification in biological nitrogen removal: From enrichment to application DOI
Zheng-Zhe Zhang, Yu Zhang, Yinguang Chen

et al.

Bioresource Technology, Journal Year: 2019, Volume and Issue: 298, P. 122444 - 122444

Published: Nov. 19, 2019

Language: Английский

Citations

175
Genomic structure predicts metabolite dynamics in microbial communities DOI Creative Commons
Karna Gowda,

Derek Ping,

Madhav Mani

et al.

Cell, Journal Year: 2022, Volume and Issue: 185(3), P. 530 - 546.e25

Published: Jan. 31, 2022

Language: Английский

Citations

97
Unlocking bacterial potential to reduce farmland N2O emissions DOI Creative Commons
Elisabeth Gautefall Hiis, Silas H. W. Vick, Lars Molstad

et al.

Nature, Journal Year: 2024, Volume and Issue: 630(8016), P. 421 - 428

Published: May 29, 2024

Abstract Farmed soils contribute substantially to global warming by emitting N 2 O (ref. 1 ), and mitigation has proved difficult . Several microbial nitrogen transformations produce O, but the only biological sink for is enzyme NosZ, catalysing reduction of 3 ). Although strengthening NosZ activity in would reduce emissions, such bioengineering soil microbiota considered challenging 4,5 However, we have developed a technology achieve this, using organic waste as substrate vector O-respiring bacteria selected their capacity thrive 6–8 Here analysed biokinetics our most promising bacterium, Cloacibacterium sp. CB-01, its survival effect on emissions field experiments. Fertilization with from biogas production, which CB-01 had grown aerobically about 6 × 10 9 cells per millilitre, reduced 50–95%, depending type. The strong long-lasting ascribed tenacity soil, rather than biokinetic parameters, were inferior those other strains bacteria. Scaling data up European level, find that national anthropogenic could be 5–20%, more if including wastes. This opens an avenue cost-effective options are lacking at present.

Language: Английский

Citations

38
Intermediate soil acidification induces highest nitrous oxide emissions DOI Creative Commons
Yunpeng Qiu, Yi Zhang, Kangcheng Zhang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 27, 2024

Abstract Global potent greenhouse gas nitrous oxide (N 2 O) emissions from soil are accelerating, with increases in the proportion of reactive nitrogen emitted as N O, i.e., O emission factor (EF). Yet, primary controls and underlying mechanisms EFs remain unresolved. Based on two independent but complementary global syntheses, three field studies determining effects acidity denitrifying microorganisms, we show that pH predominantly by affecting denitrifier community composition. Analysis 5438 paired data points fluxes revealed a hump-shaped relationship between EFs, highest occurring moderately acidic soils favored O-producing over O-consuming induced high emissions. Our results illustrate has unimodal denitrifiers net depends both O/(N + ) ratio overall denitrification rate. These findings can inform strategies to predict mitigate under future input scenarios.

Language: Английский

Citations

33
Denitrification as an N2O sink DOI Creative Commons
Mónica Conthe, Pawel Lycus, Magnus Ø. Arntzen

et al.

Water Research, Journal Year: 2018, Volume and Issue: 151, P. 381 - 387

Published: Dec. 24, 2018

The strong greenhouse gas nitrous oxide (N2O) can be emitted from wastewater treatment systems as a byproduct of ammonium oxidation and the last intermediate in stepwise reduction nitrate to N2 by denitrifying organisms. A potential strategy reduce N2O emissions would enhance activity reductase (NOS) microbial community. survey existing literature on denitrification showed that reducing capacity (VmaxN2O→N2) exceeded produce (VmaxNO3→N2O) factor 2–10. This suggests an effective sink for N2O, potentially scavenging fraction produced or abiotic reactions. We conducted series incubation experiments with freshly sampled activated sludge system Oslo found ratio α = VmaxN2O→N2/VmaxNO3→N2O fluctuated between 2 5 samples taken at intervals over period weeks. Adding cocktail carbon substrates resulted increasing rates, but had no significant effect α. Based these results – complemented qPCR metaproteomic data we discuss whether overcapacity ascribed gene/protein abundance ratios (nosZ/nir), in-cell competition reductases electrons could greater importance.

Language: Английский

Citations

141
Beyond denitrification: The role of microbial diversity in controlling nitrous oxide reduction and soil nitrous oxide emissions DOI
Jun Shan, Robert A. Sanford, Joanne C. Chee‐Sanford

et al.

Global Change Biology, Journal Year: 2021, Volume and Issue: 27(12), P. 2669 - 2683

Published: Feb. 6, 2021

Abstract Many biotic and abiotic processes contribute to nitrous oxide (N 2 O) production in the biosphere, but N O consumption environment has heretofore been attributed primarily canonical denitrifying microorganisms. The nosZ genes encoding reductase enzyme, NosZ, responsible for reduction dinitrogen are now known include two distinct groups: well‐studied Clade I which denitrifiers typically possess, novel II possessed by diverse groups of microorganisms, most non‐denitrifiers. reducers could play an important, previously unrecognized role controlling emissions several reasons, including: (1) produced other than denitrification, (2) hypothesized non‐respiratory functions NosZ as electron sink or detoxification, (3) possible differing enzyme kinetics compared (4) greater gene abundance soils many ecosystems. Despite potential ecological significance a census 800 peer‐reviewed original research articles discussing published from 2013 2019 showed that percentage evaluating mentioning increased 5% only 22% 2019. revealed slowly spreading awareness may result part disciplinary silos, with ranging 0% Agriculture Agronomy journals 32% Multidisciplinary Sciences journals. In addition, inconsistent nomenclature , 17 different terminologies used literature, have created confusion about reducers. We provide recommendations accelerate advances understanding diversity regulating soil emissions.

Language: Английский

Citations

94
A bet-hedging strategy for denitrifying bacteria curtails their release of N 2 O DOI Creative Commons
Pawel Lycus, Manuel J. Soriano‐Laguna, Morten Kjos

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2018, Volume and Issue: 115(46), P. 11820 - 11825

Published: Nov. 1, 2018

When oxygen becomes limiting, denitrifying bacteria must prepare for anaerobic respiration by synthesizing the reductases NAR (NO3- → NO2-), NIR (NO2- NO), NOR (2NO N2O), and NOS (N2O N2), either en bloc or sequentially, to avoid entrapment in anoxia without energy. Minimizing metabolic burden of this precaution is a plausible fitness trait, we show that model denitrifier Paracoccus denitrificans achieves all cells, while only minority synthesize NIR. Phenotypic diversification with regards ascribed stochastic initiation gene transcription, which autocatalytic via NO production. Observed gas kinetics suggest such bet hedging widespread among bacteria. Moreover, response oxygenation, P. preserves poles nongrowing persister ready switch sudden anoxia. Our findings add dimensions regulatory biology denitrification identify traits decrease N2O emissions.

Language: Английский

Citations

92
The anammox coupled partial-denitrification process in an integrated granular sludge and fixed-biofilm reactor developed for mainstream wastewater treatment: Performance and community structure DOI

Jinlong Zhuang,

Xu Sun,

Wei-Qi Zhao

et al.

Water Research, Journal Year: 2021, Volume and Issue: 210, P. 117964 - 117964

Published: Dec. 15, 2021

Language: Английский

Citations

83
Sustained nitrogen loss in a symbiotic association of Comammox Nitrospira and Anammox bacteria DOI Creative Commons
Ekaterina Y. Gottshall, Samuel Bryson, Kathryn I. Cogert

et al.

Water Research, Journal Year: 2021, Volume and Issue: 202, P. 117426 - 117426

Published: July 11, 2021

Language: Английский

Citations

77
Linking meta-omics to the kinetics of denitrification intermediates reveals pH-dependent causes of N2O emissions and nitrite accumulation in soil DOI Creative Commons
Åsa Frostegård, Silas H. W. Vick,

Natalie Y. N. Lim

et al.

The ISME Journal, Journal Year: 2021, Volume and Issue: 16(1), P. 26 - 37

Published: July 1, 2021

Soil pH is a key controller of denitrification. We analysed the metagenomics/transcriptomics and phenomics two soils from long-term liming experiment, SoilN (pH 6.8) un-limed SoilA 3.8). had severely delayed N

Language: Английский

Citations

67