Tomographic phase microscopy: principles and applications in bioimaging [Invited]

Journal of the Optical Society of America B, Journal Year: 2017, Volume and Issue: 34(5), P. B64 - B64

Published: April 3, 2017

Tomographic phase microscopy (TPM) is an emerging optical microscopic technique for bioimaging. TPM uses digital holographic measurements of complex scattered fields to reconstruct three-dimensional refractive index (RI) maps cells with diffraction-limited resolution by solving inverse scattering problems. In this paper, we review the developments from fundamental physics its applications in We first provide a comprehensive description tomographic reconstruction physical models used TPM. The RI map algorithms and various regularization methods are discussed. Selected cellular imaging, particularly hematology, reviewed. Finally, examine limitations current systems, propose future solutions, envision promising directions biomedical research.

Language: Английский

---

Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation

Nature Cell Biology, Journal Year: 2019, Volume and Issue: 21(4), P. 430 - 441

Published: April 1, 2019

Language: Английский

---

Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine

ACS Nano, Journal Year: 2022, Volume and Issue: 16(8), P. 11516 - 11544

Published: Aug. 2, 2022

Quantitative phase imaging (QPI) is a label-free, wide-field microscopy approach with significant opportunities for biomedical applications. QPI uses the natural shift of light as it passes through transparent object, such mammalian cell, to quantify biomass distribution and spatial temporal changes in biomass. Reported cell studies more than 60 years ago, ongoing advances hardware software are leading numerous applications biology, dramatic expansion utility over past two decades. Today, investigations size, morphology, behavior, cellular viscoelasticity, drug efficacy, accumulation turnover, transport mechanics supporting development, physiology, neural activity, cancer, additional physiological processes diseases. Here, we review field biology starting underlying principles, followed by discussion technical approaches currently available or being developed, end an examination breadth use under development. We comment on strengths shortcomings deployment key contexts conclude emerging challenges based combining other methodologies that expand scope even further.

Language: Английский

---

Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(17)

Published: April 22, 2022

Significance We report a quantitative Raman microscopy method that measures the concentration of protein and lipid in cells at high spatial resolution living fixed samples tissues, allowing studies cell size organelle regulation both culture tissue slices; it can be applied to problems control, intracellular crowding, metabolism context growth, differentiation, senescence, pathology.

Language: Английский

---

Cell size homeostasis is tightly controlled throughout the cell cycle

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(1), P. e3002453 - e3002453

Published: Jan. 5, 2024

To achieve a stable size distribution over multiple generations, proliferating cells require means of counteracting stochastic noise in the rate growth, time spent various phases cell cycle, and imprecision placement plane division. In most widely accepted model, is thought to be regulated at G1/S transition, such that smaller than critical pause end G1 phase until they have accumulated mass predetermined threshold, which point proceed through rest cycle. However, based solely on specific checkpoint G1/S, cannot readily explain why with deficient control mechanisms are still able maintain very distribution. Furthermore, model would not easily account for variation during subsequent anticipated G1/S. address questions, we applied computationally enhanced quantitative microscopy (ceQPM) populations cultured human lines, enables highly accurate measurement dry individual throughout From these measurements, evaluated factors contribute maintaining homeostasis any Our findings reveal accurately maintained, despite disruptions normal machinery or perturbations growth. Control generally confined regulation length. Instead imposed lines examined, find coefficient (CV) population begins decline well before transition continues S G2 phases. Among different types tested, detailed response growth differs. general, when it falls below exponential natural increase CV effectively constrained. We both mass-dependent cycle modulation reducing within population. Through interplay coordination 2 processes, emerges. Such previously unappreciated general principles cells. These same regulatory processes might also operative terminally differentiated Further dynamical studies should lead better understanding underlying molecular control.

Language: Английский

---

Tissue engineering strategies for promoting vascularized bone regeneration

Bone, Journal Year: 2015, Volume and Issue: 83, P. 197 - 209

Published: Nov. 19, 2015

Language: Английский

---

Dual pathways to endochondral osteoblasts: a novel chondrocyte-derived osteoprogenitor cell identified in hypertrophic cartilage

Biology Open, Journal Year: 2015, Volume and Issue: 4(5), P. 608 - 621

Published: April 17, 2015

According to the general understanding, chondrocyte lineage terminates with elimination of late hypertrophic cells by apoptosis in growth plate. However, recent cell tracking studies have shown that murine chondrocytes can survive beyond “terminal” differentiation and give rise a progeny osteoblasts participating endochondral bone formation. The question how convert into osteoblasts, however, remained open. Following fate genetic tracing using BACCol10;Cre induced YFP-reporter gene expression we show Col10Cre-reporter labelled osteoprogenitor appears primary spongiosa participates – depending on developmental stage substantially trabecular, endosteal, cortical YFP+ trabecular endosteal isolated FACS expressed Col1a1, osteocalcin runx2, thus confirming their osteogenic phenotype. In searching for transitory between identified confocal microscopy novel, small YFP+Osx+ type mitotic activity lower zone at chondro-osseous junction. When from plates fractional enzymatic digestion, these termed CDOP (chondrocyte-derived osteoprogenitor) typical genes differentiated vitro. We propose Col10Cre-labeled mark initiation point second pathway giving alternative perichondrium derived cells. These findings add current concepts chondrocyte-osteocyte lineages new insight complex cartilage-bone transition process

Language: Английский

---

Limb development: a paradigm of gene regulation

Nature Reviews Genetics, Journal Year: 2017, Volume and Issue: 18(4), P. 245 - 258

Published: Feb. 6, 2017

Language: Английский

---

IGF-1 regulation of key signaling pathways in bone

BoneKEy Reports, Journal Year: 2013, Volume and Issue: 2

Published: Oct. 2, 2013

Language: Английский

---

Quantitative phase imaging for medical diagnosis

Journal of Biophotonics, Journal Year: 2016, Volume and Issue: 10(2), P. 177 - 205

Published: Aug. 19, 2016

Optical microscopy is an indispensable diagnostic tool in modern healthcare. As a prime example, pathologists rely exclusively on light to investigate tissue morphology order make diagnosis. While advances and contrast markers allow visualize cells tissues unprecedented detail, the interpretation of these images remains largely subjective, leading inter‐ intra‐observer discrepancy. Furthermore, conventional capture qualitative information which makes it difficult automate process, reducing throughput achievable workflow. Quantitative Phase Imaging (QPI) techniques have been advanced recent years address two challenges. By quantifying physical parameters tissues, systems remove subjectivity from disease diagnosis process for easier automation increase throughput. In addition providing quantitative information, QPI are also label‐free can be easily assimilated into current workflow clinic. this paper we review made by techniques. We focus areas hematological cancer pathology, where most significant date. [Image adapted Y. Park, M. Diez‐Silva, G. Popescu, Lykotrafitis, W. Choi, S. Feld, Suresh, Proc. Natl. Acad. Sci. 105, 13730–13735 (2008).] magnified image

Language: Английский

---