The ISME Journal,
Journal Year:
2020,
Volume and Issue:
14(4), P. 1030 - 1041
Published: Jan. 27, 2020
Abstract
Anaerobic
oxidation
of
methane
(AOM)
is
a
major
biological
process
that
reduces
global
emission
to
the
atmosphere.
methanotrophic
archaea
(ANME)
mediate
this
through
coupling
different
electron
acceptors,
or
in
concert
with
syntrophic
bacterial
partner.
Recently,
ANME
belonging
archaeal
family
Methanoperedenaceae
(formerly
known
as
ANME-2d)
were
shown
be
capable
AOM
coupled
nitrate
and
iron
reduction.
Here,
freshwater
sediment
bioreactor
fed
Mn(IV)
oxides
(birnessite)
resulted
microbial
community
dominated
by
two
novel
members
Methanoperedenaceae,
biochemical
profiling
system
demonstrating
Mn(IV)-dependent
AOM.
Genomic
transcriptomic
analyses
revealed
expression
key
genes
involved
several
shared
multiheme
c-type
cytochromes
(MHCs)
differentially
expressed,
indicating
likely
use
extracellular
transfer
pathways.
We
propose
names
“Candidatus
Methanoperedens
manganicus”
manganireducens”
for
newly
described
species.
This
study
demonstrates
ability
couple
reduction
oxides,
which
suggests
their
potential
role
linking
manganese
cycling
environment.
Environmental Science & Technology,
Journal Year:
2015,
Volume and Issue:
49(7), P. 4001 - 4018
Published: Feb. 27, 2015
Methane
is
an
abundant
gas
used
in
energy
recovery
systems,
heating,
and
transport.
Methanotrophs
are
bacteria
capable
of
using
methane
as
their
sole
carbon
source.
Although
intensively
researched,
the
myriad
potential
biotechnological
applications
methanotrophic
has
not
been
comprehensively
discussed
a
single
review.
can
generate
single-cell
protein,
biopolymers,
components
for
nanotechnology
(surface
layers),
soluble
metabolites
(methanol,
formaldehyde,
organic
acids,
ectoine),
lipids
(biodiesel
health
supplements),
growth
media,
vitamin
B12
They
may
be
genetically
engineered
to
produce
new
compounds
such
carotenoids
or
farnesene.
Some
enzymes
(dehydrogenases,
oxidase,
catalase)
valuable
products
with
high
conversion
efficiencies
methanol
sequester
CO2
formic
acid
ex
vivo.
Live
cultures
bioremediation,
chemical
transformation
(propene
propylene
oxide),
wastewater
denitrification,
biosensors,
possibly
directly
generating
electricity.
This
review
demonstrates
methanotrophs
consortia
value
while
While
there
notable
challenges
low
solubility
source,
massive
resource,
cost
savings
sequestering
greenhouse
gas,
keeps
interest
piqued
these
unique
bacteria.
Scientific Reports,
Journal Year:
2016,
Volume and Issue:
6(1)
Published: Sept. 27, 2016
Abstract
H
2
metabolism
is
proposed
to
be
the
most
ancient
and
diverse
mechanism
of
energy-conservation.
The
metalloenzymes
mediating
this
metabolism,
hydrogenases,
are
encoded
by
over
60
microbial
phyla
present
in
all
major
ecosystems.
We
developed
a
classification
system
web
tool,
HydDB,
for
structural
functional
analysis
these
enzymes.
show
that
hydrogenase
function
can
predicted
primary
sequence
alone
using
an
expanded
scheme
(comprising
29
[NiFe],
8
[FeFe]
1
[Fe]
classes)
defines
11
new
classes
with
distinct
biological
functions.
Using
scheme,
we
built
tool
rapidly
reliably
classifies
sequences
combination
k
-nearest
neighbors’
algorithms
CDD
referencing.
Demonstrating
its
capacity,
content
12
newly-sequenced
bacteria,
archaea
eukaryotes.
HydDB
provides
capacity
browse
amino
acid
3248
annotated
catalytic
subunits
also
contains
detailed
repository
physiological,
biochemical
information
about
38
defined
here.
database
classifier
freely
publicly
available
at
http://services.birc.au.dk/hyddb/
Understanding
the
structure,
functions,
activities
and
dynamics
of
microbial
communities
in
natural
environments
is
one
grand
challenges
21st
century
science.
To
address
this
challenge,
over
past
decade,
numerous
technologies
have
been
developed
for
interrogating
communities,
which
some
are
amenable
to
exploratory
work
(e.g.,
high-throughput
sequencing
phenotypic
screening)
others
depend
on
reference
genes
or
genomes
phylogenetic
functional
gene
arrays).
Here,
we
provide
a
critical
review
synthesis
most
commonly
applied
"open-format"
"closed-format"
detection
technologies.
We
discuss
their
characteristics,
advantages,
disadvantages
within
context
environmental
applications
focus
analysis
complex
systems,
such
as
those
soils,
diversity
high
few.
In
addition,
crucial
issues
considerations
associated
with
applying
complementary
molecular
important
ecological
questions.
Science,
Journal Year:
2016,
Volume and Issue:
351(6274), P. 703 - 707
Published: Feb. 12, 2016
The
oxidation
of
methane
with
sulfate
is
an
important
microbial
metabolism
in
the
global
carbon
cycle.
In
marine
seeps,
this
process
mediated
by
consortia
anaerobic
methanotrophic
archaea
(ANME)
that
live
syntrophy
sulfate-reducing
bacteria
(SRB).
underlying
interdependencies
within
uncultured
symbiotic
partnership
are
poorly
understood.
We
used
a
combination
rate
measurements
and
single-cell
stable
isotope
probing
to
demonstrate
ANME
deep-sea
sediments
can
be
catabolically
anabolically
decoupled
from
their
syntrophic
SRB
partners
using
soluble
artificial
oxidants.
still
sustain
high
rates
absence
as
terminal
oxidant,
lending
support
hypothesis
interspecies
extracellular
electron
transfer
mechanism
for
methane.
Archaea,
Journal Year:
2017,
Volume and Issue:
2017, P. 1 - 22
Published: Jan. 1, 2017
Anaerobic
oxidation
of
methane
(AOM)
is
catalyzed
by
anaerobic
methane-oxidizing
archaea
(ANME)
via
a
reverse
and
modified
methanogenesis
pathway.
Methanogens
can
also
the
pathway
to
oxidize
methane,
but
only
during
net
production
(i.e.,
"trace
oxidation").
In
turn,
ANME
produce
enzymatic
back
flux).
Net
AOM
exergonic
when
coupled
an
external
electron
acceptor
such
as
sulfate
(ANME-1,
ANME-2abc,
ANME-3),
nitrate
(ANME-2d),
or
metal
(oxides).
this
review,
reversibility
essential
differences
between
methanogens
are
described
combining
published
information
with
domain
based
(meta)genome
comparison
archaeal
methanotrophs
selected
archaea.
These
include
abundances
special
structure
methyl
coenzyme
M
reductase
multiheme
cytochromes
presence
menaquinones
methanophenazines.
ANME-2a
ANME-2d
use
acceptors
other
than
for
AOM,
respectively.
Environmental
studies
suggest
that
involved
in
sulfate-dependent
AOM.
ANME-1
seem
different
mechanism
disposal
electrons
possibly
less
versatile
ANME-2.
Future
research
will
shed
light
on
molecular
basis
reversal
methanogenic
transfer
types.
