Spatiotemporal constraints on optogenetic inactivation in cortical circuits DOI Creative Commons
Nuo Li, Susu Chen, Zengcai V. Guo

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Nov. 18, 2019

Optogenetics allows manipulations of genetically and spatially defined neuronal populations with excellent temporal control. However, neurons are coupled other over multiple length scales, the effects localized thus spread beyond targeted neurons. We benchmarked several optogenetic methods to inactivate small regions neocortex. Optogenetic excitation GABAergic produced more effective inactivation than light-gated ion pumps. Transgenic mice expressing light-dependent chloride channel GtACR1 most potent inactivation. Generally, substantially photostimulation light, caused by strong coupling between cortical Over some range light intensity, inhibitory reduced activity in these neurons, together pyramidal a signature inhibition-stabilized neural networks ('paradoxical effect'). The offset was followed rebound dose-dependent manner, limiting resolution. Our data offer guidance for design vivo optogenetics experiments.

Language: Английский

Inhibitory Plasticity: Balance, Control, and Codependence DOI Open Access
Guillaume Hennequin, Everton J. Agnes, Tim P. Vogels

et al.

Annual Review of Neuroscience, Journal Year: 2017, Volume and Issue: 40(1), P. 557 - 579

Published: June 9, 2017

Inhibitory neurons, although relatively few in number, exert powerful control over brain circuits. They stabilize network activity the face of strong feedback excitation and actively engage computations. Recent studies reveal importance a precise balance inhibition neural circuits, which often requires exquisite fine-tuning inhibitory connections. We review synaptic plasticity its roles shaping both feedforward control. discuss necessity complex, codependent mechanisms to build nontrivial, functioning networks, we end by summarizing experimental evidence such interactions.

Language: Английский

Citations

235
Visuomotor Coupling Shapes the Functional Development of Mouse Visual Cortex DOI Creative Commons

Alexander Attinger,

Bo Wang, Georg B. Keller

et al.

Cell, Journal Year: 2017, Volume and Issue: 169(7), P. 1291 - 1302.e14

Published: June 1, 2017

Language: Английский

Citations

231
Alzheimer’s Disease: From Firing Instability to Homeostasis Network Collapse DOI Creative Commons
Samuel Frère, Inna Slutsky

Neuron, Journal Year: 2018, Volume and Issue: 97(1), P. 32 - 58

Published: Jan. 1, 2018

Language: Английский

Citations

222
Rett syndrome: insights into genetic, molecular and circuit mechanisms DOI
Jacque Pak Kan Ip, Nikolaos Mellios, Mriganka Sur

et al.

Nature reviews. Neuroscience, Journal Year: 2018, Volume and Issue: 19(6), P. 368 - 382

Published: May 8, 2018

Language: Английский

Citations

215
Spatiotemporal constraints on optogenetic inactivation in cortical circuits DOI Creative Commons
Nuo Li, Susu Chen, Zengcai V. Guo

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Nov. 18, 2019

Optogenetics allows manipulations of genetically and spatially defined neuronal populations with excellent temporal control. However, neurons are coupled other over multiple length scales, the effects localized thus spread beyond targeted neurons. We benchmarked several optogenetic methods to inactivate small regions neocortex. Optogenetic excitation GABAergic produced more effective inactivation than light-gated ion pumps. Transgenic mice expressing light-dependent chloride channel GtACR1 most potent inactivation. Generally, substantially photostimulation light, caused by strong coupling between cortical Over some range light intensity, inhibitory reduced activity in these neurons, together pyramidal a signature inhibition-stabilized neural networks ('paradoxical effect'). The offset was followed rebound dose-dependent manner, limiting resolution. Our data offer guidance for design vivo optogenetics experiments.

Language: Английский

Citations

207