Proceedings of the National Academy of Sciences,
Journal Year:
2017,
Volume and Issue:
114(35)
Published: Aug. 15, 2017
Significance
CRISPR-Cas
is
an
adaptive
immunity
system
that
protects
bacteria
and
archaea
from
mobile
genetic
elements.
We
present
comparative
genomic
phylogenetic
analysis
of
minimal
variants
associated
with
distinct
families
transposable
elements
develop
the
hypothesis
such
repurposed
defense
systems
contribute
to
element
propagation
by
facilitating
transposition
into
specific
sites.
Thus,
these
are
predicted
propagate
via
RNA-guided
transposition,
a
mechanism
has
not
been
previously
described
for
DNA
transposons.
Science,
Journal Year:
2020,
Volume and Issue:
369(6501), P. 333 - 337
Published: July 17, 2020
Compact
defense
system
in
bacteriophages
The
CRISPR-Cas
system,
naturally
found
many
prokaryotes,
is
widely
used
for
genome
editing.
CRISPR
arrays
the
bacterial
genome,
derived
from
of
invading
viruses,
are
to
generate
a
RNA
that
guides
Cas
enzyme
destroy
repeat
viral
invaders.
Recently,
an
unexpectedly
compact
was
identified
huge
bacteriophages.
Pausch
et
al
.
show
even
though
this
lacks
commonly
accessory
proteins,
it
functional.
In
addition
array,
only
component
called
CasF,
which
uses
same
active
site
process
transcripts
into
and
foreign
nucleic
acids.
This
human
plant
cells,
provides
hypercompact
genome-editing
toolbox.
Science
issue
p.
333
Journal of Bacteriology,
Journal Year:
2018,
Volume and Issue:
200(7)
Published: Jan. 18, 2018
ABSTRACT
Clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)-Cas
systems
are
well-known
acquired
immunity
that
widespread
in
archaea
and
bacteria.
The
RNA-guided
nucleases
from
CRISPR-Cas
currently
regarded
as
the
most
reliable
tools
for
genome
editing
engineering.
first
hint
of
their
existence
came
1987,
when
an
unusual
repetitive
DNA
sequence,
which
subsequently
was
defined
a
CRISPR,
discovered
Escherichia
coli
during
analysis
genes
involved
phosphate
metabolism.
Similar
sequence
patterns
were
then
reported
range
other
bacteria
well
halophilic
archaea,
suggesting
important
role
such
evolutionarily
conserved
clusters
repeated
sequences.
A
critical
step
toward
functional
characterization
recognition
link
between
CRISPRs
associated
Cas
proteins,
initially
hypothesized
to
be
repair
hyperthermophilic
archaea.
Comparative
genomics,
structural
biology,
advanced
biochemistry
could
work
hand
hand,
not
only
culminating
explosion
based
on
CRISPR-Cas9
class
II
but
also
providing
insights
into
origin
evolution
this
system
mobile
genetic
elements
denoted
casposons.
To
celebrate
30th
anniversary
discovery
minireview
briefly
discusses
fascinating
history
systems,
original
observation
enigmatic
E.
humans.
