Deep learning acceleration of multiscale superresolution localization photoacoustic imaging DOI Creative Commons
Jongbeom Kim, Gyuwon Kim, Lei Li

et al.

Light Science & Applications, Journal Year: 2022, Volume and Issue: 11(1)

Published: May 12, 2022

A superresolution imaging approach that localizes very small targets, such as red blood cells or droplets of injected photoacoustic dye, has significantly improved spatial resolution in various biological and medical modalities. However, this superior is achieved by sacrificing temporal because many raw image frames, each containing the localization target, must be superimposed to form a sufficiently sampled high-density image. Here, we demonstrate computational strategy based on deep neural networks (DNNs) reconstruct images from far fewer frames. The can applied for both 3D label-free optical-resolution microscopy (OR-PAM) 2D labeled computed tomography (PACT). For former, required number volumetric frames reduced tens than ten. latter, 12 fold. Therefore, our proposed method simultaneously (via DNN) method) resolutions tomography. Deep-learning powered PA potentially provide practical tool preclinical clinical studies requiring fast fine resolutions.

Language: Английский

A practical guide to photoacoustic tomography in the life sciences DOI
Lihong V. Wang, Junjie Yao

Nature Methods, Journal Year: 2016, Volume and Issue: 13(8), P. 627 - 638

Published: July 28, 2016

Language: Английский

Citations

1187
Ultrafast ultrasound localization microscopy for deep super-resolution vascular imaging DOI

Claudia Errico,

Juliette Pierre, Sophie Pezet

et al.

Nature, Journal Year: 2015, Volume and Issue: 527(7579), P. 499 - 502

Published: Nov. 1, 2015

Language: Английский

Citations

1091
Light in diagnosis, therapy and surgery DOI
Seok Hyun Yun, Sheldon J. J. Kwok

Nature Biomedical Engineering, Journal Year: 2017, Volume and Issue: 1(1)

Published: Jan. 10, 2017

Language: Английский

Citations

671
Photoacoustic Imaging: Contrast Agents and Their Biomedical Applications DOI
Qinrui Fu,

Rong Zhu,

Jibin Song

et al.

Advanced Materials, Journal Year: 2018, Volume and Issue: 31(6)

Published: Dec. 17, 2018

Abstract Photoacoustic (PA) imaging as a fast‐developing technique has great potential in biomedical and clinical applications. It is noninvasive modality that depends on the light‐absorption coefficient of imaged tissue injected PA‐imaging contrast agents. Furthermore, PA provides superb contrast, super spatial resolution, high penetrability sensitivity to functional characteristics by detecting acoustic wave construct images. In recent years, series agents are developed improve performance Here, progress their applications outlined. classified according components function, gold nanocrystals, gold‐nanocrystal assembly, transition‐metal chalcogenides/MXene‐based nanomaterials, carbon‐based other inorganic agents, small organic molecules, semiconducting polymer nanoparticles, nonlinear discussed. The biosensors (in sensing metal ions, pH, enzymes, temperature, hypoxia, reactive oxygen species, nitrogen species) bioimaging (lymph nodes, vasculature, tumors, brain tissue) discussed detail. Finally, an outlook future research investigation significance presented.

Language: Английский

Citations

572
Single-impulse panoramic photoacoustic computed tomography of small-animal whole-body dynamics at high spatiotemporal resolution DOI
Lei Li, Liren Zhu, Cheng Ma

et al.

Nature Biomedical Engineering, Journal Year: 2017, Volume and Issue: 1(5)

Published: May 10, 2017

Language: Английский

Citations

419
Highly efficient photothermal nanoagent achieved by harvesting energy via excited-state intramolecular motion within nanoparticles DOI Creative Commons
Zheng Zhao, Chao Chen, Wenting Wu

et al.

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Feb. 15, 2019

The exciting applications of molecular motion are still limited and in urgent pursuit, although some fascinating concepts such as motors machines have been proposed for years. Utilizing a nanoplatform practical application has scarcely explored due to unconquered challenges how achieve effective the aggregate state within nanoparticles. Here, we introduce class near infrared-absorbing organic molecules with intramolecular motion-induced photothermy inside nanoparticles, which enables most absorbed light energy dissipate heat. Such property makes nanoparticles superior photoacoustic imaging agent compared widely used methylene blue semiconducting polymer allow them high-contrast tumours live mice. This study not only provides strategy developing advanced photothermal/photoacoustic nanoagents, but also find way application.

Language: Английский

Citations

388
Tutorial on photoacoustic tomography DOI Open Access
Yong Zhou, Junjie Yao, Lihong V. Wang

et al.

Journal of Biomedical Optics, Journal Year: 2016, Volume and Issue: 21(6), P. 061007 - 061007

Published: April 18, 2016

Photoacoustic tomography (PAT) has become one of the fastest growing fields in biomedical optics. Unlike pure optical imaging, such as confocal microscopy and two-photon microscopy, PAT employs acoustic detection to image absorption contrast with high-resolution deep into scattering tissue. So far, been widely used for multiscale anatomical, functional, molecular imaging biological tissues. We focus on PAT's basic principles, major implementations, contrasts, recent applications.

Language: Английский

Citations

374
Advanced optoacoustic methods for multiscale imaging of in vivo dynamics DOI Creative Commons
Xosé Luís Deán‐Ben, Sven Gottschalk, Benedict Mc Larney

et al.

Chemical Society Reviews, Journal Year: 2017, Volume and Issue: 46(8), P. 2158 - 2198

Published: Jan. 1, 2017

Visualization of dynamic functional and molecular events in an unperturbed vivo environment is essential for understanding the complex biology living organisms disease state progression. To this end, optoacoustic (photoacoustic) sensing imaging have demonstrated exclusive capacity to maintain excellent optical contrast high resolution deep-tissue observations, far beyond penetration limits modern microscopy. Yet, time domain paramount observation study biological interactions that may be invisible single snapshots systems. This review focuses on recent advances assisted by smart labeling enhancement approaches enable new types multiscale observations not attainable with other bio-imaging modalities. A wealth investigated research topics clinical applications further discussed, including large-scale brain activity patterns, volumetric visualization moving organs agent kinetics, using targeted genetically expressed labels, as well three-dimensional handheld diagnostics human subjects.

Language: Английский

Citations

297
Photoacoustic tomography of blood oxygenation: A mini review DOI Creative Commons
Mucong Li, Yuqi Tang, Junjie Yao

et al.

Photoacoustics, Journal Year: 2018, Volume and Issue: 10, P. 65 - 73

Published: June 1, 2018

Photoacoustic tomography (PAT) is a hybrid imaging modality that combines rich contrast of optical excitation and deep penetration ultrasound detection. With its unique absorption mechanism, PAT inherently sensitive to the functional molecular information biological tissues, thus has been widely used in preclinical clinical studies. Among many capabilities PAT, measuring blood oxygenation arguably one most important applications, performed photoacoustic studies brain functions, tumor hypoxia, wound healing, cancer therapy. Yet, complex conditions especially strong wavelength-dependent attenuation, have long hurdled measurement at depths beyond few millimeters. A variety methods developed improve accuracy measurement, using novel laser illumination schemes, oxygen-sensitive fluorescent dyes, comprehensive mathematic models, or prior provided by complementary modalities. These made exciting progress, while several challenges remain. This concise review aims introduce recent developments compare each method's advantages limitations, highlight their representative discuss remaining for future advances.

Language: Английский

Citations

285
Review on practical photoacoustic microscopy DOI Creative Commons
Seungwan Jeon, Jongbeom Kim, Donghyun Lee

et al.

Photoacoustics, Journal Year: 2019, Volume and Issue: 15, P. 100141 - 100141

Published: Aug. 9, 2019

Photoacoustic imaging (PAI) has many interesting advantages, such as deep depth, high image resolution, and contrast to intrinsic extrinsic chromophores, enabling morphological, functional, molecular of living subjects. microscopy (PAM) is one form the PAI inheriting its characteristics useful in both preclinical clinical research. Over years, PAM systems have been evolved several forms each relative advantages disadvantages. Thus, maximize benefits for a specific application, it important configure system optimally by targeting application. In this review, we provide practical methods implementing improve signal-to-noise ratio (SNR), speed. addition, review applications discuss current challenges scope future developments.

Language: Английский

Citations

279