Lanthanide-Based Optical Probes of Biological Systems DOI Creative Commons
Ukrae Cho, James Chen

Cell chemical biology, Journal Year: 2020, Volume and Issue: 27(8), P. 921 - 936

Published: July 30, 2020

Language: Английский

RNA delivery by extracellular vesicles in mammalian cells and its applications DOI Open Access
Killian P. O’Brien, Koen Breyne, Stefano Ughetto

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(10), P. 585 - 606

Published: May 26, 2020

Language: Английский

Citations

1418
P-Bodies: Composition, Properties, and Functions DOI Creative Commons
Yang Luo,

Zhenkun Na,

Sarah A. Slavoff

et al.

Biochemistry, Journal Year: 2018, Volume and Issue: 57(17), P. 2424 - 2431

Published: Jan. 30, 2018

Processing bodies (P-bodies) are cytoplasmic ribonucleoprotein (RNP) granules primarily composed of translationally repressed mRNAs and proteins related to mRNA decay, suggesting roles in post-transcriptional regulation. P-bodies conserved eukaryotic cells exhibit properties liquid droplets. However, the function translational repression and/or decay remains contentious. Here we review recent advances our understanding molecular composition P-bodies, interactions processes that regulate P-body liquid–liquid phase separation (LLPS), cellular localization machinery, context how these discoveries refine models function.

Language: Английский

Citations

526
Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs DOI
Xianjun Chen, Dasheng Zhang,

Ni Su

et al.

Nature Biotechnology, Journal Year: 2019, Volume and Issue: 37(11), P. 1287 - 1293

Published: Sept. 23, 2019

Language: Английский

Citations

296
Intracellular mRNA transport and localized translation DOI
Sulagna Das, Mariá Vera, Valentina Gandin

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2021, Volume and Issue: 22(7), P. 483 - 504

Published: April 9, 2021

Language: Английский

Citations

272
A Growing Toolbox to Image Gene Expression in Single Cells: Sensitive Approaches for Demanding Challenges DOI Creative Commons
Xavier Pichon, Mounia Lagha, Florian Mueller

et al.

Molecular Cell, Journal Year: 2018, Volume and Issue: 71(3), P. 468 - 480

Published: Aug. 1, 2018

Language: Английский

Citations

174
Illuminating RNA biology through imaging DOI Open Access
Phuong Le, Noorsher Ahmed,

G Yeo

et al.

Nature Cell Biology, Journal Year: 2022, Volume and Issue: 24(6), P. 815 - 824

Published: June 1, 2022

Language: Английский

Citations

77
A rapid inducible RNA decay system reveals fast mRNA decay in P-bodies DOI Creative Commons
Lauren A. Blake, Leslie Watkins, Yang Liu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 28, 2024

Abstract RNA decay is vital for regulating mRNA abundance and gene expression. Existing technologies lack the spatiotemporal precision or transcript specificity to capture stochastic transient process. We devise a general strategy inducibly recruit protein factors modulate target metabolism. Specifically, we introduce Rapid Inducible Decay of (RIDR) technology degrade mRNAs within minutes. The fast synchronous induction enables direct visualization dynamics in cells. Applying RIDR endogenous ACTB reveals rapid formation dissolution granules pre-existing P-bodies. Time-resolved distribution measurements demonstrate inside P-bodies, which further supported by knocking down P-body constituent proteins. Light oxidative stress behavior, potentially reconciling contradictory literature about function. This study compartmentalized kinetics, establishing as pivotal tool exploring metabolism

Language: Английский

Citations

19
Real-time single-molecule imaging of transcriptional regulatory networks in living cells DOI
Dong-Woo Hwang, Anna Maekiniemi, Robert H. Singer

et al.

Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: 25(4), P. 272 - 285

Published: Jan. 9, 2024

Language: Английский

Citations

18
Imaging the dynamics of messenger RNA with a bright and stable green fluorescent RNA DOI
Fangting Zuo, Li Jiang,

Ni Su

et al.

Nature Chemical Biology, Journal Year: 2024, Volume and Issue: 20(10), P. 1272 - 1281

Published: May 23, 2024

Language: Английский

Citations

18
A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells DOI
Esther Braselmann, Aleksandra J. Wierzba, Jacob T. Polaski

et al.

Nature Chemical Biology, Journal Year: 2018, Volume and Issue: 14(10), P. 964 - 971

Published: July 27, 2018

Language: Английский

Citations

141