Pan-European study of genotypes and phenotypes in the Arabidopsis relative Cardamine hirsuta reveals how adaptation, demography, and development shape diversity patterns

PLoS Biology, Journal Year: 2023, Volume and Issue: 21(7), P. e3002191 - e3002191

Published: July 18, 2023

We study natural DNA polymorphisms and associated phenotypes in the Arabidopsis relative Cardamine hirsuta . observed strong genetic differentiation among several ancestry groups broader distribution of Iberian relict strains European C compared to Arabidopsis. found synchronization between vegetative reproductive development a pervasive role for heterochronic pathways shaping variation. A single, fast-cycling ChFRIGIDA allele evolved adaptively allowing range expansion from glacial refugia, unlike where multiple FRIGIDA haplotypes were involved. The Azores islands, is scarce, are hotspot diversity. identified quantitative trait locus (QTL) SPL9 transcription factor as determinant an Azorean morphotype. This QTL shows evidence positive selection, its mirrors climate gradient that broadly shaped flora. Overall, we establish framework explore how interplay adaptation, demography, diversity patterns 2 related plant species.

Language: Английский

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Amphicarpic development in Cardamine chenopodiifolia

New Phytologist, Journal Year: 2024, Volume and Issue: 244(3), P. 1041 - 1056

Published: July 18, 2024

Summary Amphicarpy is an unusual trait where two fruit types develop on the same plant: one above and other belowground. This not found in conventional model species. Therefore, its development molecular genetics remain under‐studied. Here, we establish allooctoploid Cardamine chenopodiifolia as emerging experimental system to study amphicarpy. We characterized C. development, focusing differences morphology cell wall histochemistry between above‐ belowground fruit. generated a reference transcriptome with PacBio full‐length transcript sequencing analysed differential gene expression valves. has contrasting modes of seed dispersal. The main shoot fails bolt initiates floral primordia that grow underground they self‐pollinate set seed. By contrast, axillary shoots exploding pods aboveground. Morphological aerial explosive subterranean nonexplosive were reflected large number differentially regulated genes involved photosynthesis, secondary formation defence responses. Tools established , such transcriptome, draft genome assembly stable plant transformation, pave way amphicarpy evolution via allopolyploidy.

Language: Английский

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The genomes of two Eutrema species provide insight into plant adaptation to high altitudes

DNA Research, Journal Year: 2018, Volume and Issue: 25(3), P. 307 - 315

Published: Jan. 26, 2018

Eutrema is a genus in the Brassicaceae, which includes species of scientific and economic importance. Many are montane and/or alpine that arose very recently, making them ideal candidates for comparative studies to understand both ecological speciation high-altitude adaptation plants. Here we provide de novo whole-genome assemblies pair recently diverged perennials with contrasting altitude preferences, E. heterophyllum from eastern Qinghai-Tibet Plateau its lowland congener yunnanense. The two assembled genomes 350 Mb 412 Mb, respectively, 29,606 28,881 predicted genes. Comparative analysis revealed demographic trajectories evolution gene families. Gene family expansions shared between other were identified, including disease resistance R genes (NBS-LRRs or NLRs). Genes duplicated specifically involved mainly reproduction, DNA damage repair cold tolerance. reported here constitute important genetic resources diverse studies, Eutrema, Brassicaceae as whole plants across world.

Language: Английский

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Genome-Wide Identification and Expression Analysis of the Cation Diffusion Facilitator Gene Family in Turnip Under Diverse Metal Ion Stresses

Frontiers in Genetics, Journal Year: 2018, Volume and Issue: 9

Published: April 4, 2018

The cation diffusion facilitator (CDF) family is one of the gene families involved in metal ion uptake and transport plants, but understanding definite roles mechanisms most CDF genes remain limited. In present study, we identified 18 candidate from turnip genome named them BrrMTP1.1–BrrMTP12. Then, performed a comparative genomic analysis on phylogenetic relationships, structures chromosome distributions, conserved domains motifs CDFs. constructed tree indicated that BrrMTPs were divided into seven groups (groups 1, 5, 6, 7, 8, 9 12) formed three major clusters (Zn-CDFs, Fe/Zn-CDFs Mn-CDFs). Moreover, structural characteristics BrrMTP members same group similar varied among groups. To investigate potential turnip, conducted an expression all under Mg, Zn, Cu, Mn, Fe, Co, Na Cd stresses. Results showed levels induced by at least ion, indicating these may be related to tolerance or those ions. Basing different ions for hypothesized are possibly heavy accumulation salt stress apart their maintenance mineral nutrient homeostasis turnip. These findings helpful understand MTPs plants provide preliminary information study functions genes.

Language: Английский

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Genetic dissection of the auxin response network

Nature Plants, Journal Year: 2020, Volume and Issue: 6(9), P. 1082 - 1090

Published: Aug. 17, 2020

Language: Английский

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