Genome biology,
Journal Year:
2020,
Volume and Issue:
21(1)
Published: Feb. 7, 2020
Abstract
The
recent
boom
in
microfluidics
and
combinatorial
indexing
strategies,
combined
with
low
sequencing
costs,
has
empowered
single-cell
technology.
Thousands—or
even
millions—of
cells
analyzed
a
single
experiment
amount
to
data
revolution
biology
pose
unique
science
problems.
Here,
we
outline
eleven
challenges
that
will
be
central
bringing
this
emerging
field
of
forward.
For
each
challenge,
highlight
motivating
research
questions,
review
prior
work,
formulate
open
This
compendium
is
for
established
researchers,
newcomers,
students
alike,
highlighting
interesting
rewarding
problems
the
coming
years.
Science,
Journal Year:
2018,
Volume and Issue:
361(6406), P. 1033 - 1037
Published: Sept. 6, 2018
Metastases
are
responsible
for
the
majority
of
cancer-related
deaths.
Although
genomic
heterogeneity
within
primary
tumors
is
associated
with
relapse,
among
treatment-naïve
metastases
has
not
been
comprehensively
assessed.
We
analyzed
sequencing
data
76
untreated
from
20
patients
and
inferred
cancer
phylogenies
breast,
colorectal,
endometrial,
gastric,
lung,
melanoma,
pancreatic,
prostate
cancers.
found
that
individual
patients,
a
large
driver
gene
mutations
common
to
all
metastases.
Further
analysis
revealed
were
shared
by
unlikely
have
functional
consequences.
A
mathematical
model
tumor
evolution
metastasis
formation
provides
an
explanation
observed
homogeneity.
Thus,
single
biopsies
capture
most
functionally
important
in
therefore
provide
essential
information
therapeutic
decision-making.
