RNA,
Journal Year:
2017,
Volume and Issue:
23(12), P. 1754 - 1769
Published: Aug. 30, 2017
RNA
modifications
have
been
historically
considered
as
fine-tuning
chemo-structural
features
of
infrastructural
RNAs,
such
rRNAs,
tRNAs,
and
snoRNAs.
This
view
has
changed
dramatically
in
recent
years,
to
a
large
extent
result
systematic
efforts
map
quantify
various
transcriptome-wide
manner,
revealing
that
are
reversible,
dynamically
regulated,
far
more
widespread
than
originally
thought,
involved
major
biological
processes,
including
cell
differentiation,
sex
determination,
stress
responses.
Here
we
summarize
the
state
knowledge
provide
catalog
their
links
neurological
disorders,
cancers,
other
diseases.
With
advent
direct
RNA-sequencing
technologies,
expect
this
will
help
prioritize
those
for
maps.
Signal Transduction and Targeted Therapy,
Journal Year:
2021,
Volume and Issue:
6(1)
Published: Feb. 21, 2021
Abstract
N
6
-methyladenosine
(m6A)
is
the
most
prevalent,
abundant
and
conserved
internal
cotranscriptional
modification
in
eukaryotic
RNAs,
especially
within
higher
cells.
m6A
modified
by
methyltransferases,
or
writers,
such
as
METTL3/14/16,
RBM15/15B,
ZC3H3,
VIRMA,
CBLL1,
WTAP,
KIAA1429,
and,
removed
demethylases,
erasers,
including
FTO
ALKBH5.
It
recognized
m6A-binding
proteins
YTHDF1/2/3,
YTHDC1/2
IGF2BP1/2/3
HNRNPA2B1,
also
known
“readers”.
Recent
studies
have
shown
that
RNA
plays
essential
role
both
physiological
pathological
conditions,
initiation
progression
of
different
types
human
cancers.
In
this
review,
we
discuss
how
methylation
influences
progressions
hematopoietic,
central
nervous
reproductive
systems.
We
will
mainly
focus
on
recent
progress
identifying
biological
functions
underlying
molecular
mechanisms
methylation,
its
regulators
downstream
target
genes,
during
cancer
above
propose
process
offer
potential
targets
for
therapy
future.
Cell Research,
Journal Year:
2018,
Volume and Issue:
28(6), P. 616 - 624
Published: May 22, 2018
Abstract
N
6
-methyladenosine
(m
A)
is
a
chemical
modification
present
in
multiple
RNA
species,
being
most
abundant
mRNAs.
Studies
on
enzymes
or
factors
that
catalyze,
recognize,
and
remove
m
A
have
revealed
its
comprehensive
roles
almost
every
aspect
of
mRNA
metabolism,
as
well
variety
physiological
processes.
This
review
describes
the
current
understanding
modification,
particularly
functions
writers,
erasers,
readers
with
an
emphasis
role
regulating
isoform
dosage
Hepatology,
Journal Year:
2017,
Volume and Issue:
67(6), P. 2254 - 2270
Published: Nov. 24, 2017
Epigenetic
alterations
have
contributed
greatly
to
human
carcinogenesis.
Conventional
epigenetic
studies
predominantly
focused
on
DNA
methylation,
histone
modifications,
and
chromatin
remodeling.
Recently,
diverse
reversible
chemical
modifications
of
RNAs
emerged
as
a
new
layer
regulation.
N6‐methyladenosine
(m6A)
is
the
most
abundant
modification
eukaryotic
messenger
RNA
(mRNA)
important
for
regulation
mRNA
stability,
splicing,
translation.
Using
transcriptome
sequencing,
we
discovered
that
methyltransferase‐like
3
(METTL3),
major
N6‐adenosine
methyltransferase,
was
significantly
up‐regulated
in
hepatocellular
carcinoma
(HCC)
multiple
solid
tumors.
Clinically,
overexpression
METTL3
associated
with
poor
prognosis
patients
HCC.
Functionally,
proved
knockdown
drastically
reduced
HCC
cell
proliferation,
migration,
colony
formation
vitro
.
Knockout
remarkably
suppressed
tumorigenicity
lung
metastasis
vivo
On
other
hand,
using
CRISPR/dCas9‐VP64
activation
system,
demonstrated
promoted
growth
both
Through
m6A
methylated
immuno‐precipitation
quantitative
reverse‐transcription
polymerase
chain
reaction,
identified
suppressor
cytokine
signaling
2
(SOCS2)
target
METTL3‐mediated
modification.
Knockdown
substantially
abolished
SOCS2
augmented
expression.
We
also
showed
m6A‐mediated
degradation
relied
reader
protein
YTHDF2‐dependent
pathway.
Conclusion
:
frequently
contributes
progression.
represses
expression
through
an
m6A‐YTHDF2‐dependent
mechanism.
Our
findings
suggest
mechanism
alteration
liver
(H
epatology
2018;67:2254‐2270).
Molecular Cancer,
Journal Year:
2019,
Volume and Issue:
18(1)
Published: May 29, 2019
N6-methyladenosine
(m6A)
is
identified
as
the
most
common,
abundant
and
conserved
internal
transcriptional
modification,
especially
within
eukaryotic
messenger
RNAs
(mRNAs).
M6A
modification
installed
by
m6A
methyltransferases
(METTL3/14,
WTAP,
RBM15/15B
KIAA1429,
termed
"writers"),
reverted
demethylases
(FTO
ALKBH5,
"erasers")
recognized
binding
proteins
(YTHDF1/2/3,
IGF2BP1
HNRNPA2B1,
"readers").
Acumulating
evidence
shows
that,
RNA
methylation
has
an
outsize
effect
on
production/metabolism
participates
in
pathogenesis
of
multiple
diseases
including
cancers.
Until
now,
molecular
mechanisms
underlying
various
tumors
have
not
been
comprehensively
clarified.
In
this
review,
we
mainly
summarize
recent
advances
biological
function
modifications
human
cancer
discuss
potential
therapeutic
strategies.
