Calcium Signaling Mechanisms Across Kingdoms

Annual Review of Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 37(1), P. 311 - 340

Published: Aug. 10, 2021

Calcium (Ca 2+ ) is a unique mineral that serves as both nutrient and signal in all eukaryotes. To maintain Ca homeostasis for nutrition signaling purposes, the tool kit transport has expanded across kingdoms of eukaryotes to encode specific signals referred signatures. In parallel, large array -binding proteins evolved sensors decode By comparing these coding decoding mechanisms fungi, animals, plants, unified divergent themes have emerged, underlying complexity will challenge researchers years come. Considering scale breadth subject, instead literature survey, this review we focus on conceptual framework aims introduce readers principles signaling. We finish with several examples -signaling pathways, including polarized cell growth, immunity symbiosis, systemic signaling, piece together plants versus animals.

Language: Английский

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Tethering Piezo channels to the actin cytoskeleton for mechanogating via the cadherin-β-catenin mechanotransduction complex

Cell Reports, Journal Year: 2022, Volume and Issue: 38(6), P. 110342 - 110342

Published: Feb. 1, 2022

The mechanically activated Piezo channel plays a versatile role in conferring mechanosensitivity to various cell types. However, how it incorporates its intrinsic and cellular components effectively sense long-range mechanical perturbation across remains elusive. Here we show that channels are biochemically functionally tethered the actin cytoskeleton via cadherin-β-catenin mechanotransduction complex, whose significantly impairs Piezo-mediated responses. Mechanistically, adhesive extracellular domain of E-cadherin interacts with cap Piezo1, which controls transmembrane gate, while cytosolic tail might interact domains close proximity intracellular gates, allowing direct focus adhesion-cytoskeleton-transmitted force for gating. Specific disruption intermolecular interactions prevents cytoskeleton-dependent gating Piezo1. Thus, propose force-from-filament model complement previously suggested force-from-lipids mechanogating channels, enabling them serve as tunable mechanotransducers.

Language: Английский

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Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction

Frontiers in Bioengineering and Biotechnology, Journal Year: 2019, Volume and Issue: 7

Published: July 16, 2019

Cells and tissues can sense react to the modifications of physico-chemical properties extracellular environment (ECM) through integrin-based adhesion sites adapt their physiological response in a process called mechanotransduction. Due critical localization at cell-ECM interface, transmembrane integrins are mediators bidirectional signaling, playing key role « outside-in » inside-out signal transduction. After presenting basic conceptual fundamentals related field mechanobiology, we review current state-of-the-art technologies that facilitate understanding mechanotransduction signaling pathways. Finally, highlight innovative technological developments help advance our molecular mechanisms involved nuclear

Language: Английский

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Red Blood Cells: Chasing Interactions

Frontiers in Physiology, Journal Year: 2019, Volume and Issue: 10

Published: July 31, 2019

Human red blood cells (RBC) are highly differentiated that have lost all organelles and most intracellular machineries during their maturation process. RBC fundamental for the nearly basic physiologic dynamics they key in body's respiratory system by being responsible oxygen transport to tissues, delivery of carbon dioxide lungs. With flexible structure capable deform order travel through vessels including very small capillaries. Throughout average 120 days lifespan, human bloodstream come contact with a broad range different cell types. In fact, able interact communicate endothelial (ECs), platelets, macrophages bacteria. Additionally, involved maintenance thrombosis hemostasis play an important role immune response against pathogens. To clarify mechanisms interaction these other both health disease as well highlight players, we focused our interest on membrane components such ion channels, proteins, phospholipids.

Language: Английский

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Mechanically activated piezo channels modulate outflow tract valve development through the Yap1 and Klf2-Notch signaling axis

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Sept. 16, 2019

Mechanical forces are well known for modulating heart valve developmental programs. Yet, it is still unclear how genetic programs and mechanosensation interact during development. Here, we assessed the mechanosensitive pathways involved zebrafish outflow tract (OFT) development in vivo. Our results show that hippo effector Yap1, Klf2, Notch signaling pathway all essential OFT morphogenesis response to mechanical forces, albeit active different cell layers. Furthermore, Piezo TRP channels important factors these pathways. In addition, live reporters reveal controls Klf2 activity endothelium Yap1 localization smooth muscle progenitors coordinate morphogenesis. Together, this work identifies a unique morphogenetic program formation places as central modulator of process.

Language: Английский

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