Chemical Reviews,
Journal Year:
2022,
Volume and Issue:
122(9), P. 9145 - 9197
Published: April 8, 2022
Proteins
that
self-assemble
into
polyhedral
shell-like
structures
are
useful
molecular
containers
both
in
nature
and
the
laboratory.
Here
we
review
efforts
to
repurpose
diverse
protein
cages,
including
viral
capsids,
ferritins,
bacterial
microcompartments,
designed
capsules,
as
vaccines,
drug
delivery
vehicles,
targeted
imaging
agents,
nanoreactors,
templates
for
controlled
materials
synthesis,
building
blocks
higher-order
architectures,
more.
A
deep
understanding
of
principles
underlying
construction,
function,
evolution
natural
systems
has
been
key
tailoring
selective
cargo
encapsulation
interactions
with
biological
synthetic
through
engineering
directed
evolution.
The
ability
adapt
design
increasingly
sophisticated
capsid
functions
stands
benefit
fields
catalysis,
science,
medicine.
Nature Biotechnology,
Journal Year:
2022,
Volume and Issue:
41(4), P. 488 - 499
Published: Oct. 10, 2022
Abstract
Large
serine
recombinases
(LSRs)
are
DNA
integrases
that
facilitate
the
site-specific
integration
of
mobile
genetic
elements
into
bacterial
genomes.
Only
a
few
LSRs,
such
as
Bxb1
and
PhiC31,
have
been
characterized
to
date,
with
limited
efficiency
tools
for
in
human
cells.
In
this
study,
we
developed
computational
approach
identify
thousands
LSRs
their
attachment
sites,
expanding
known
LSR
diversity
by
>100-fold
enabling
prediction
insertion
site
specificities.
We
tested
recombination
activity
cells,
classifying
them
landing
pad,
genome-targeting
or
multi-targeting
LSRs.
Overall,
achieved
up
seven-fold
higher
than
genome
efficiencies
40–75%
cargo
sizes
over
7
kb.
also
demonstrate
virus-free,
direct
plasmid
amplicon
libraries
improved
functional
genomics
applications.
This
systematic
discovery
directly
from
microbial
sequencing
data
provides
resource
60
experimentally
cells
large-payload
without
exposed
double-stranded
breaks.
Pharmaceutics,
Journal Year:
2022,
Volume and Issue:
14(2), P. 427 - 427
Published: Feb. 16, 2022
Bacterial
biofilms
formed
by
pathogens
are
known
to
be
hundreds
of
times
more
resistant
antimicrobial
agents
than
planktonic
cells,
making
it
extremely
difficult
cure
biofilm-based
infections
despite
the
use
antibiotics,
which
poses
a
serious
threat
human
health.
Therefore,
there
is
an
urgent
need
develop
promising
alternative
therapies
reduce
burden
drug-resistant
bacterial
caused
biofilms.
As
natural
enemies
bacteria,
bacteriophages
(phages)
have
advantages
high
specificity,
safety
and
non-toxicity,
possess
great
potential
in
defense
removal
pathogenic
biofilms,
considered
alternatives
treat
diseases.
This
work
mainly
reviews
composition,
structure
formation
process
briefly
discusses
interaction
between
phages
summarizes
several
strategies
based
on
their
derivatives
against
serving
purpose
developing
novel,
safe
effective
treatment
methods
promoting
application
maintaining
Chemical Reviews,
Journal Year:
2022,
Volume and Issue:
122(9), P. 9145 - 9197
Published: April 8, 2022
Proteins
that
self-assemble
into
polyhedral
shell-like
structures
are
useful
molecular
containers
both
in
nature
and
the
laboratory.
Here
we
review
efforts
to
repurpose
diverse
protein
cages,
including
viral
capsids,
ferritins,
bacterial
microcompartments,
designed
capsules,
as
vaccines,
drug
delivery
vehicles,
targeted
imaging
agents,
nanoreactors,
templates
for
controlled
materials
synthesis,
building
blocks
higher-order
architectures,
more.
A
deep
understanding
of
principles
underlying
construction,
function,
evolution
natural
systems
has
been
key
tailoring
selective
cargo
encapsulation
interactions
with
biological
synthetic
through
engineering
directed
evolution.
The
ability
adapt
design
increasingly
sophisticated
capsid
functions
stands
benefit
fields
catalysis,
science,
medicine.
