Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

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Chemoconnectomics: Mapping Chemical Transmission in Drosophila

Neuron, Journal Year: 2019, Volume and Issue: 101(5), P. 876 - 893.e4

Published: Feb. 21, 2019

Language: Английский

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Mouse Retinal Cell Atlas: Molecular Identification of over Sixty Amacrine Cell Types

Journal of Neuroscience, Journal Year: 2020, Volume and Issue: 40(27), P. 5177 - 5195

Published: May 26, 2020

Amacrine cells (ACs) are a diverse class of interneurons that modulate input from photoreceptors to retinal ganglion (RGCs), rendering each RGC type selectively sensitive particular visual features, which then relayed the brain. While many AC types have been identified morphologically and physiologically, they not comprehensively classified or molecularly characterized. We used high-throughput single-cell RNA sequencing profile >32,000 ACs mice both sexes applied computational methods identify 63 types. molecular markers for them characterize morphology multiple show include nearly all previously known as well had described. Consistent with previous studies, most expressed canonical inhibitory neurotransmitters GABA glycine, but several neither both. In addition, one more neuropeptides, two glutamatergic markers. also explored transcriptomic relationships among transcription factors by individual closely related Noteworthy these were Meis2 Tcf4, GABAergic glycinergic types, respectively. Together, results provide foundation developmental functional studies ACs, means genetically accessing them. Along molecular, physiological, morphologic analyses, establish existence at least 130 neuronal 140 cell in mouse retina. SIGNIFICANCE STATEMENT The retina is leading model analyzing development, structure, function, pathology neural circuits. A complete atlas provides an important studies. heterogeneous interneurons, amacrine cells, identifying distinct includes novel evidence use groups Combining those obtained previously, we proposed contains ∼130 therefore comparable complexity other regions

Language: Английский

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CGRP physiology, pharmacology, and therapeutic targets: migraine and beyond

Physiological Reviews, Journal Year: 2022, Volume and Issue: 103(2), P. 1565 - 1644

Published: Dec. 1, 2022

Calcitonin gene-related peptide (CGRP) is a neuropeptide with diverse physiological functions. Its two isoforms (α and β) are widely expressed throughout the body in sensory neurons as well other cell types, such motor neuroendocrine cells. CGRP acts via at least G protein-coupled receptors that form unusual complexes receptor activity-modifying proteins. These AMY

Language: Английский

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Ion Channel Degeneracy, Variability, and Covariation in Neuron and Circuit Resilience

Annual Review of Neuroscience, Journal Year: 2021, Volume and Issue: 44(1), P. 335 - 357

Published: March 26, 2021

The large number of ion channels found in all nervous systems poses fundamental questions concerning how the characteristic intrinsic properties single neurons are determined by specific subsets they express. All display many different with overlapping voltage- and time-dependent properties. We speculate that these promote resilience neuronal function. Individual same cell type show variability channel conductance densities even though can generate reliable similar behavior. This complicates a simple assignment function to any is associated variable responses perturbations, deletions, pharmacological manipulation. Ion genes often strong positively correlated expression, which may result from molecular developmental rules determine expressed given type.

Language: Английский

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A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord

eLife, Journal Year: 2020, Volume and Issue: 9

Published: April 21, 2020

The Drosophila ventral nerve cord (VNC) receives and processes descending signals from the brain to produce a variety of coordinated locomotor outputs. It also integrates sensory information periphery sends ascending brain. We used single-cell transcriptomics generate an unbiased classification cellular diversity in VNC five-day old adult flies. produced atlas 26,000 high-quality cells, representing more than 100 transcriptionally distinct cell types. predominant gene signatures defining neuronal types reflect shared developmental histories based on neuroblast which cells were derived, as well their birth order. relative position along anterior-posterior axis could be assigned using Hox expression. This transcriptional fly will valuable resource for future studies neurodevelopment behavior.

Language: Английский

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Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster

Cell, Journal Year: 2024, Volume and Issue: 187(10), P. 2574 - 2594.e23

Published: May 1, 2024

High-resolution electron microscopy of nervous systems has enabled the reconstruction synaptic connectomes. However, we do not know sign for each connection (i.e., whether a is excitatory or inhibitory), which implied by released transmitter. We demonstrate that artificial neural networks can predict transmitter types presynapses from micrographs: network trained to six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy 87% individual synapses, 94% neurons, and 91% known cell across D. melanogaster whole brain. visualize ultrastructural features used prediction, discovering subtle but significant differences between phenotypes. also analyze distributions brain find neurons develop together largely express only one fast-acting GABA). hope our publicly available predictions act as accelerant neuroscientific hypothesis generation fly.

Language: Английский

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Basal forebrain cholinergic signalling: development, connectivity and roles in cognition

Nature reviews. Neuroscience, Journal Year: 2023, Volume and Issue: 24(4), P. 233 - 251

Published: Feb. 23, 2023

Language: Английский

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Spatial transcriptomics reveal neuron–astrocyte synergy in long-term memory

Nature, Journal Year: 2024, Volume and Issue: 627(8003), P. 374 - 381

Published: Feb. 7, 2024

Abstract Memory encodes past experiences, thereby enabling future plans. The basolateral amygdala is a centre of salience networks that underlie emotional experiences and thus has key role in long-term fear memory formation 1 . Here we used spatial single-cell transcriptomics to illuminate the cellular molecular architecture memory. We identified transcriptional signatures subpopulations neurons astrocytes were memory-specific persisted for weeks. These implicate neuropeptide BDNF signalling, MAPK CREB activation, ubiquitination pathways, synaptic connectivity as components Notably, upon formation, neuronal subpopulation defined by increased Penk decreased Tac expression constituted most prominent component engram amygdala. changes observed both with RNA sequencing single-molecule intact slices, providing rich map engram. data enabled us determine this interacts adjacent astrocytes, functional experiments show require interactions encode

Language: Английский

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Synaptic and circuit functions of multitransmitter neurons in the mammalian brain

Neuron, Journal Year: 2023, Volume and Issue: 111(19), P. 2969 - 2983

Published: July 17, 2023

Language: Английский

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