Recent advances in electrochemical nanomaterial-based aptasensors for the detection of cancer biomarkers

Talanta, Journal Year: 2023, Volume and Issue: 259, P. 124548 - 124548

Published: April 11, 2023

Language: Английский

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Exosomal circTGFBR2 promotes hepatocellular carcinoma progression via enhancing ATG5 mediated protective autophagy

Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(7)

Published: July 20, 2023

Abstract Exosomes contribute substantially to the communication between tumor cells and normal cells. Benefiting from stable structure, circular RNAs (circRNAs) are believed serve an important function in exosome-mediated intercellular communication. Here, we focused on circRNAs enriched starvation-stressed hepatocytic exosomes further investigated their mechanism hepatocellular carcinoma (HCC) progression. Differentially expressed were identified by RNA sequencing, circTGFBR2 was chosen for study. The molecular of HCC demonstrated pulldown, RIP, dual-luciferase reporter assays, rescue experiments xenograft assay both vitro vivo. We confirmed with led upregulated resistance starvation stress. Mechanistically, delivered into via serves as a competing endogenous binding miR-205-5p facilitate ATG5 expression enhance autophagy cells, resulting starvation. Thus, revealed that is novel promoter circRNA promotes progression enhancing ATG5–mediated protective circTGFBR2/miR-205-5p/ATG5 axis, which may be potential therapeutic target HCC.

Language: Английский

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miR-142-3p encapsulated in T lymphocyte-derived tissue small extracellular vesicles induces Treg function defect and thyrocyte destruction in Hashimoto’s thyroiditis

BMC Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: June 6, 2023

Hashimoto's thyroiditis (HT) is an organ-specific autoimmune disease characterized by lymphocyte infiltration that destroys thyrocyte cells. The aim of the present study was to elucidate role and mechanisms tissue small extracellular vesicle (sEV) microRNAs (miRNAs) in pathogenesis HT.Differentially expressed sEV miRNAs were identified between HT normal RNA sequencing testing set (n = 20). Subsequently, using quantitative real-time polymerase chain reaction (qRT‒PCR) assays logistic regression analysis validation 60), most relevant verified. parental recipient cells miRNA then explored. In vitro vivo experiments further performed function potential contribute development HT.We miR-142-3p encapsulated T lymphocyte-derived sEVs can induce Treg defect destruction through intact response loop. Inactivation effectively protect non-obese diabetic (NOD).H-2h4 mice from display reduced infiltration, lower antibody titers, higher Looking at underlying action on destruction, we found strong deleterious effect mediated due its ability block activation ERK1/2 signaling pathway downregulating RAC1.Our findings highlight fact sEV-mediated transfer serve as a communication mode lymphocytes HT, favoring progression HT.

Language: Английский

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Melt electrowriting (MEW)-PCL composite Three-Dimensional exosome hydrogel scaffold for wound healing

Materials & Design, Journal Year: 2024, Volume and Issue: 238, P. 112717 - 112717

Published: Jan. 29, 2024

While mesenchymal stem cell-derived exosomes hold substantial potential in wound healing, challenges persist terms of large-scale production and activity 2D-culture derived exosome, as well addressing their inactivation loss during application. 3D can be produced more efficiently possess higher activity. However, there lacks a delivery patch mimicking nanofibrous architecture the extracellular matrix while facilitating situ exosomes, thereby minimizing dissipation accelerating process healing. In this study, we devised controllable GelMA hydrogel-combined Melt Electrowriting (MEW)-PCL scaffold for 3D-exosome release. We showed that biocompatible scaffolds prepared by MEW have simulated with highly arrangement nanofibers support cell adhesion, proliferation differentiation. Through proliferation, scratch assay, tube formation experiments, verified could effectively stimulate migration, formation, dose-dependent effects. vivo outcomes exhibited accelerated re-epithelialization, improved collagen maturation, enhanced angiogenesis. Our findings suggest 3D-cultured within significantly enhance repair. This innovative strategy opens up new avenues application MSC-derived

Language: Английский

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Extracellular vesicles isolated from Arabidopsis thaliana leaves reveal characteristics of mammalian exosomes

PROTOPLASMA, Journal Year: 2024, Volume and Issue: 261(5), P. 1025 - 1033

Published: April 29, 2024

Language: Английский

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