Exploring the Biological Impact of β-TCP Surface Polarization on Osteoblast and Osteoclast Activity DOI Open Access

Jingpu Zheng,

Kosuke Nozaki, Kazuaki Hashimoto

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 141 - 141

Published: Dec. 27, 2024

β-tricalcium phosphate (β-TCP) is a widely utilized resorbable bone graft material, whose surface charge can be modified by electrical polarization. However, the specific effects of such modification on osteoblast and osteoclast functions remain insufficiently studied. In this work, electrically polarized β-TCP with high density was synthesized evaluated in vitro terms its physicochemical properties biological activity. Polarization performed to achieve density, which quantified using thermally stimulated depolarization current. The proliferation differentiation MC3T3-E1 osteoblast-like cells were assessed via WST-8 alkaline phosphatase assays. Tartrate-resistant acid (TRAP) activity resorption pit assay used evaluate impact RAW264.7 osteoclast-like cell Polarized exhibited 1.3 mC cm−2. Electrically surfaces significantly enhanced differentiation. TRAP assays demonstrated effective cells, observed charged surfaces. Resorption further revealed improved capacity charge. These findings indicate that highly dense promotes differentiation, as well capacity.

Language: Английский

Innovative 3D printing technologies and advanced materials revolutionizing orthopedic surgery: current applications and future directions DOI Creative Commons
Bo Cong,

Haiguang Zhang

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 11, 2025

Three-dimensional (3D) printing has rapidly become a transformative force in orthopedic surgery, enabling the creation of highly customized and precise medical implants surgical tools. This review aims to provide more systematic comprehensive perspective on emerging 3D technologies—ranging from extrusion-based methods bioink powder bed fusion—and broadening array materials, including bioactive agents cell-laden inks. We highlight how these technologies materials are employed fabricate patient-specific implants, guides, prosthetics, advanced tissue engineering scaffolds, significantly enhancing outcomes patient recovery. Despite notable progress, field faces challenges such as optimizing mechanical properties, ensuring structural integrity, addressing regulatory complexities across different regions, considering environmental impacts cost barriers, especially low-resource settings. Looking ahead, innovations smart functionally graded (FGMs), along with advancements bioprinting, hold promise for overcoming obstacles expanding capabilities orthopedics. underscores pivotal role interdisciplinary collaboration ongoing research harnessing full potential additive manufacturing, ultimately paving way effective, personalized, durable solutions that improve quality life.

Language: Английский

Citations

5
The effect of sterilization methods on the cytotoxicity of ceramic medical implants DOI Creative Commons
A. R. Bilyalov, Svetlana Piatnitskaia, Guzel Rafikova

et al.

Bulletin of Russian State Medical University, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Feb. 1, 2025

The choice of the sterilization method for ceramic implants is critically important, as it can affect chemical and physico-mechanical properties material its biocompatibility. Higher cytotoxicity, which a possible side effect sterilization, hinders osseointegration. This study aimed to determine cytotoxicity porous samples after using most common methods. Samples hydroxyapatite (HA), tricalcium phosphate (TCP), aluminum oxide (AO) were prepared by stereolithography, bone allograph made DLP method. annealing lasted 4 hours, with peak temperature 800 °C increment 3 per minute; sintering was up 1200 °C. We used following methods: autoclaving at 1 atmosphere, 120 °C, 45 minutes; radiation 25 seconds an absorbed dose kGy; plasma peroxide 42 dry heat 180 60 minutes. Cytotoxicity determined help MTT assay (24-hour exposure in CO2 incubator). results study: HA, high porosity means growth values transition from (0.1115) (0.2023). Medium low show similar results, peaks dry-heat (0.4954 0.4505). As AO, exhibited viability when subjected this TCP have shown stable but their low-porosity variation had growing (0.078 0.182, sterilization). forms basis optimizing manufacturing technology methods ensure

Language: Английский

Citations

0
Knochenersatz - synthetische Materialien in der dentalen Implantologie DOI

Sebastian Spintzyk,

Jeremias Hey,

Felix Tilsen

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1), P. 22 - 25

Published: March 1, 2025

Citations

0
4D Bioprinting for Personalized Medicine, Innovations in Implant Fabrication and Regenerative Therapies DOI

Senthil Maharaj Kennedy,

Manigandan Sekar,

A. Vasanthanathan

et al.

Polymer-Plastics Technology and Materials, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 26

Published: March 30, 2025

Language: Английский

Citations

0
Printing β-TCP-laden GelMA/Alginate Interpenetrating-Polymer-Network Biomaterial Inks for Bone Tissue Engineering DOI
Joyce Rodrigues de Souza, Maedeh Rahimnejad, Igor Paulino Mendes Soares

et al.

Bioprinting, Journal Year: 2025, Volume and Issue: unknown, P. e00413 - e00413

Published: April 1, 2025

Language: Английский

Citations

0
Milestones in Mandibular Bone Tissue Engineering: A Systematic Review of Large Animal Models and Critical-Sized Defects DOI Open Access
Yannick M. Sillmann,

Pascal Eber,

Elizabeth Orbeta

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(8), P. 2717 - 2717

Published: April 15, 2025

Background/Objectives: Mandibular reconstruction following trauma or oncologic resection is crucial for restoring function and aesthetics. While autologous bone grafting remains the gold standard, it presents challenges such as donor site morbidity graft availability. Bone tissue engineering (BTE) offers an innovative alternative, integrating scaffolds, osteogenic cells, bioactive factors to regenerate functional bone. This systematic review evaluates BTE strategies mandibular reconstruction, focusing on critical-sized defects in large animal models their translational potential clinical applications. Methods: A was performed PRISMA guidelines. Eligible studies involved treated with at least two components (scaffold, growth factors). Quality bias assessments were conducted using ARRIVE guidelines SYRCLE tools. Results: Of 6088 screened, 27 met inclusion criteria, pigs, sheep, dogs. Common scaffolds included β-tricalcium phosphate (β-TCP), poly-lactic-co-glycolic acid (PLGA), polycaprolactone (PCL), frequently combined marrow-derived mesenchymal stem cells (BMSCs) like recombinant human morphogenetic protein-2 (rhBMP-2). Preclinical outcomes demonstrated effective regeneration, vascularization, biomechanical restoration. Advanced strategies, including vivo bioreactors 3D-printed further enhanced regeneration. However, incomplete scaffold degradation, hypoxic conditions within constructs, variability factor efficacy dose optimization observed, emphasizing need refinement ensure consistent outcomes. Conclusions: shows promise achieving regeneration restoration preclinical of defects. optimization, vascularization enhancement, protocol standardization require investigation facilitate translation. These findings emphasize achieve consistent, scalable use.

Language: Английский

Citations

0
Insight into bioactive glass and bio-ceramics uses: unveiling recent advances for biomedical application DOI Creative Commons
Doha El‐Sayed Ellakwa,

Ahmad S. Abu‐Khadra,

Takwa E. Ellakwa

et al.

Discover Materials, Journal Year: 2025, Volume and Issue: 5(1)

Published: April 25, 2025

Language: Английский

Citations

0
Beyond 3D Printing: How AI is Shaping the Future of Craniomaxillofacial Bone Tissue Engineering DOI

Pascal Eber,

Yannick M. Sillmann, Fernando Pozzi Semeghini Guastaldi

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

This perspective focuses on the potential of artificial intelligence (AI) in craniomaxillofacial (CMF) bone tissue engineering, mitigating current challenges, and driving development tailored biomaterials clinical translation. CMF engineering faces significant challenges due to complexity defects, limitations traditional grafting methods, need for precise anatomical reconstruction. AI is revolutionizing by leveraging vast computational power analyze complex biological data, optimize treatment strategies, enhance next-generation regenerative solutions. facilitates customization scaffolds patient-specific enables implementation drug delivery systems controlled therapeutic release, drives innovative with improved biocompatibility, enhances reproducibility precision scaffold fabrication, advances new additive technologies, such as AI-driven 3D 4D printing, manufacturing accuracy efficiency. Furthermore, accelerates diagnostics predictive modeling, enabling more effective decision-making planning improving long-term outcomes. Required standardized, updated protocols significantly improve transparency effectively bridge gap between preclinical research application, ensuring consistent validation translation innovations. By integrating medicine, paving way personalized efficient solutions reconstruction, offering transformative advancements patient care shaping future medicine therapies.

Language: Английский

Citations

0
A comprehensive review of additively manufactured biomedical titanium-based alloys for bone tissue engineering: Biocorrosion, biomechanical, and biological properties DOI Creative Commons
Amir Hossein Noorbakhsh Nezhad, E. Mohammadi Zahrani,

Saba Mousavinasab

et al.

Journal of Materials Research and Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Biomimetic design of advanced ceramics for hard tissue repair DOI
Kai Lü,

Yuekui Xu,

Shi-Jie Gao

et al.

Journal of the American Ceramic Society, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Abstract The field of biomimetic ceramics has gained considerable attention due to their potential applications in hard tissue repair, owing ability replicate the complex structures and mechanical properties found natural biological systems. This review explores role starting with an examination biomineralization processes nature that inspire development novel ceramic materials. microstructural features tissues, such as bone enamel, are highlighted emphasize relevance design effective ceramics. Various fabrication methods, including porosity‐inducing techniques, freeze casting, cold sintering, additive manufacturing, discussed detail, a focus on advantages for creating functional scaffolds. Additionally, emerging machine learning designing optimizing is explored, showcasing how computational models algorithms predict material properties, improve processes, accelerate innovation biomaterials. Furthermore, this covers current excellent well advanced materials promote angiogenesis, nerve environmental responsiveness, supporting regeneration at multiple levels. article provides insights into significance vast ceramics, offering valuable resource researchers interdisciplinary fields biomedical engineering, science, life sciences.

Language: Английский

Citations

0