International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
26(1), P. 141 - 141
Published: Dec. 27, 2024
β-tricalcium
phosphate
(β-TCP)
is
a
widely
utilized
resorbable
bone
graft
material,
whose
surface
charge
can
be
modified
by
electrical
polarization.
However,
the
specific
effects
of
such
modification
on
osteoblast
and
osteoclast
functions
remain
insufficiently
studied.
In
this
work,
electrically
polarized
β-TCP
with
high
density
was
synthesized
evaluated
in
vitro
terms
its
physicochemical
properties
biological
activity.
Polarization
performed
to
achieve
density,
which
quantified
using
thermally
stimulated
depolarization
current.
The
proliferation
differentiation
MC3T3-E1
osteoblast-like
cells
were
assessed
via
WST-8
alkaline
phosphatase
assays.
Tartrate-resistant
acid
(TRAP)
activity
resorption
pit
assay
used
evaluate
impact
RAW264.7
osteoclast-like
cell
Polarized
exhibited
1.3
mC
cm−2.
Electrically
surfaces
significantly
enhanced
differentiation.
TRAP
assays
demonstrated
effective
cells,
observed
charged
surfaces.
Resorption
further
revealed
improved
capacity
charge.
These
findings
indicate
that
highly
dense
promotes
differentiation,
as
well
capacity.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2025,
Volume and Issue:
13
Published: Feb. 11, 2025
Three-dimensional
(3D)
printing
has
rapidly
become
a
transformative
force
in
orthopedic
surgery,
enabling
the
creation
of
highly
customized
and
precise
medical
implants
surgical
tools.
This
review
aims
to
provide
more
systematic
comprehensive
perspective
on
emerging
3D
technologies—ranging
from
extrusion-based
methods
bioink
powder
bed
fusion—and
broadening
array
materials,
including
bioactive
agents
cell-laden
inks.
We
highlight
how
these
technologies
materials
are
employed
fabricate
patient-specific
implants,
guides,
prosthetics,
advanced
tissue
engineering
scaffolds,
significantly
enhancing
outcomes
patient
recovery.
Despite
notable
progress,
field
faces
challenges
such
as
optimizing
mechanical
properties,
ensuring
structural
integrity,
addressing
regulatory
complexities
across
different
regions,
considering
environmental
impacts
cost
barriers,
especially
low-resource
settings.
Looking
ahead,
innovations
smart
functionally
graded
(FGMs),
along
with
advancements
bioprinting,
hold
promise
for
overcoming
obstacles
expanding
capabilities
orthopedics.
underscores
pivotal
role
interdisciplinary
collaboration
ongoing
research
harnessing
full
potential
additive
manufacturing,
ultimately
paving
way
effective,
personalized,
durable
solutions
that
improve
quality
life.
Bulletin of Russian State Medical University,
Journal Year:
2025,
Volume and Issue:
2025(1)
Published: Feb. 1, 2025
The
choice
of
the
sterilization
method
for
ceramic
implants
is
critically
important,
as
it
can
affect
chemical
and
physico-mechanical
properties
material
its
biocompatibility.
Higher
cytotoxicity,
which
a
possible
side
effect
sterilization,
hinders
osseointegration.
This
study
aimed
to
determine
cytotoxicity
porous
samples
after
using
most
common
methods.
Samples
hydroxyapatite
(HA),
tricalcium
phosphate
(TCP),
aluminum
oxide
(AO)
were
prepared
by
stereolithography,
bone
allograph
made
DLP
method.
annealing
lasted
4
hours,
with
peak
temperature
800
°C
increment
3
per
minute;
sintering
was
up
1200
°C.
We
used
following
methods:
autoclaving
at
1
atmosphere,
120
°C,
45
minutes;
radiation
25
seconds
an
absorbed
dose
kGy;
plasma
peroxide
42
dry
heat
180
60
minutes.
Cytotoxicity
determined
help
MTT
assay
(24-hour
exposure
in
CO2
incubator).
results
study:
HA,
high
porosity
means
growth
values
transition
from
(0.1115)
(0.2023).
Medium
low
show
similar
results,
peaks
dry-heat
(0.4954
0.4505).
As
AO,
exhibited
viability
when
subjected
this
TCP
have
shown
stable
but
their
low-porosity
variation
had
growing
(0.078
0.182,
sterilization).
forms
basis
optimizing
manufacturing
technology
methods
ensure
Journal of Clinical Medicine,
Journal Year:
2025,
Volume and Issue:
14(8), P. 2717 - 2717
Published: April 15, 2025
Background/Objectives:
Mandibular
reconstruction
following
trauma
or
oncologic
resection
is
crucial
for
restoring
function
and
aesthetics.
While
autologous
bone
grafting
remains
the
gold
standard,
it
presents
challenges
such
as
donor
site
morbidity
graft
availability.
Bone
tissue
engineering
(BTE)
offers
an
innovative
alternative,
integrating
scaffolds,
osteogenic
cells,
bioactive
factors
to
regenerate
functional
bone.
This
systematic
review
evaluates
BTE
strategies
mandibular
reconstruction,
focusing
on
critical-sized
defects
in
large
animal
models
their
translational
potential
clinical
applications.
Methods:
A
was
performed
PRISMA
guidelines.
Eligible
studies
involved
treated
with
at
least
two
components
(scaffold,
growth
factors).
Quality
bias
assessments
were
conducted
using
ARRIVE
guidelines
SYRCLE
tools.
Results:
Of
6088
screened,
27
met
inclusion
criteria,
pigs,
sheep,
dogs.
Common
scaffolds
included
β-tricalcium
phosphate
(β-TCP),
poly-lactic-co-glycolic
acid
(PLGA),
polycaprolactone
(PCL),
frequently
combined
marrow-derived
mesenchymal
stem
cells
(BMSCs)
like
recombinant
human
morphogenetic
protein-2
(rhBMP-2).
Preclinical
outcomes
demonstrated
effective
regeneration,
vascularization,
biomechanical
restoration.
Advanced
strategies,
including
vivo
bioreactors
3D-printed
further
enhanced
regeneration.
However,
incomplete
scaffold
degradation,
hypoxic
conditions
within
constructs,
variability
factor
efficacy
dose
optimization
observed,
emphasizing
need
refinement
ensure
consistent
outcomes.
Conclusions:
shows
promise
achieving
regeneration
restoration
preclinical
of
defects.
optimization,
vascularization
enhancement,
protocol
standardization
require
investigation
facilitate
translation.
These
findings
emphasize
achieve
consistent,
scalable
use.
This
perspective
focuses
on
the
potential
of
artificial
intelligence
(AI)
in
craniomaxillofacial
(CMF)
bone
tissue
engineering,
mitigating
current
challenges,
and
driving
development
tailored
biomaterials
clinical
translation.
CMF
engineering
faces
significant
challenges
due
to
complexity
defects,
limitations
traditional
grafting
methods,
need
for
precise
anatomical
reconstruction.
AI
is
revolutionizing
by
leveraging
vast
computational
power
analyze
complex
biological
data,
optimize
treatment
strategies,
enhance
next-generation
regenerative
solutions.
facilitates
customization
scaffolds
patient-specific
enables
implementation
drug
delivery
systems
controlled
therapeutic
release,
drives
innovative
with
improved
biocompatibility,
enhances
reproducibility
precision
scaffold
fabrication,
advances
new
additive
technologies,
such
as
AI-driven
3D
4D
printing,
manufacturing
accuracy
efficiency.
Furthermore,
accelerates
diagnostics
predictive
modeling,
enabling
more
effective
decision-making
planning
improving
long-term
outcomes.
Required
standardized,
updated
protocols
significantly
improve
transparency
effectively
bridge
gap
between
preclinical
research
application,
ensuring
consistent
validation
translation
innovations.
By
integrating
medicine,
paving
way
personalized
efficient
solutions
reconstruction,
offering
transformative
advancements
patient
care
shaping
future
medicine
therapies.
Journal of the American Ceramic Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 7, 2025
Abstract
The
field
of
biomimetic
ceramics
has
gained
considerable
attention
due
to
their
potential
applications
in
hard
tissue
repair,
owing
ability
replicate
the
complex
structures
and
mechanical
properties
found
natural
biological
systems.
This
review
explores
role
starting
with
an
examination
biomineralization
processes
nature
that
inspire
development
novel
ceramic
materials.
microstructural
features
tissues,
such
as
bone
enamel,
are
highlighted
emphasize
relevance
design
effective
ceramics.
Various
fabrication
methods,
including
porosity‐inducing
techniques,
freeze
casting,
cold
sintering,
additive
manufacturing,
discussed
detail,
a
focus
on
advantages
for
creating
functional
scaffolds.
Additionally,
emerging
machine
learning
designing
optimizing
is
explored,
showcasing
how
computational
models
algorithms
predict
material
properties,
improve
processes,
accelerate
innovation
biomaterials.
Furthermore,
this
covers
current
excellent
well
advanced
materials
promote
angiogenesis,
nerve
environmental
responsiveness,
supporting
regeneration
at
multiple
levels.
article
provides
insights
into
significance
vast
ceramics,
offering
valuable
resource
researchers
interdisciplinary
fields
biomedical
engineering,
science,
life
sciences.