Dual-wavelength UV-visible metalens for multispectral photoacoustic microscopy: A simulation study DOI Creative Commons
Aleksandr Barulin, Hyemi Park, Byullee Park

et al.

Photoacoustics, Journal Year: 2023, Volume and Issue: 32, P. 100545 - 100545

Published: Aug. 1, 2023

Photoacoustic microscopy is advancing with research on utilizing ultraviolet and visible light. Dual-wavelength approaches are sought for observing DNA/RNA- vascular-related disorders. However, the availability of high numerical aperture lenses covering both wavelengths severely limited due to challenges such as chromatic aberration in optics. Herein, we present a groundbreaking proposal pioneering simulation study incorporating multilayer metalenses into ultraviolet-visible photoacoustic microscopy. The proposed metalens has thickness 1.4 µm 0.8. By arranging cylindrical hafnium oxide nanopillars, design an achromatic transmissive lens 266 532 nm wavelengths. achieves diffraction-limited focal spot, surpassing commercially available objective lenses. Through three-dimensional simulation, demonstrate high-resolution imaging superior endogenous contrast targets optical absorption bands. This will open new possibilities downsized multispectral clinical preclinical applications.

Language: Английский

Recent Advances in Contrast-Enhanced Photoacoustic Imaging: Overcoming the Physical and Practical Challenges DOI
Wonseok Choi, Byullee Park, Seongwook Choi

et al.

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(11), P. 7379 - 7419

Published: Jan. 16, 2023

For decades now, photoacoustic imaging (PAI) has been investigated to realize its potential as a niche biomedical modality. Despite highly desirable optical contrast and ultrasonic spatiotemporal resolution, PAI is challenged by such physical limitations low signal-to-noise ratio (SNR), diminished image due strong attenuation, lower-bound on spatial resolution in deep tissue. In addition, contrast-enhanced faced practical insufficient cell-specific targeting delivery efficiency difficulties developing clinically translatable agents. Identifying these essential the continuing expansion of field, substantial advances contrast-enhancing agents, complemented high-performance acquisition systems, have synergistically dealt with challenges conventional PAI. This review covers past four years research pushing terms SNR/contrast, targeted delivery, clinical application. Promising strategies for dealing each challenge are reviewed detail, future directions next generation discussed.

Language: Английский

Citations

122
Functional photoacoustic imaging: from nano- and micro- to macro-scale DOI Creative Commons
Byullee Park,

Donghyeon Oh,

Jeesu Kim

et al.

Nano Convergence, Journal Year: 2023, Volume and Issue: 10(1)

Published: June 19, 2023

Functional photoacoustic imaging is a promising biological technique that offers such unique benefits as scalable resolution and depth, well the ability to provide functional information. At nanoscale, has provided super-resolution images of surface light absorption characteristics materials single organelles in cells. microscopic macroscopic scales. techniques have precisely measured quantified various physiological parameters, oxygen saturation, vessel morphology, blood flow, metabolic rate oxygen, both human animal subjects. This comprehensive review provides an overview across multiple scales, from nano macro, highlights recent advances technology developments applications. Finally, surveys future prospects biomedical field.

Language: Английский

Citations

67
Deep Learning Enhances Multiparametric Dynamic Volumetric Photoacoustic Computed Tomography In Vivo (DL‐PACT) DOI Creative Commons
Seongwook Choi, Jinge Yang, Soo Young Lee

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 10(1)

Published: Nov. 10, 2022

Photoacoustic computed tomography (PACT) has become a premier preclinical and clinical imaging modality. Although PACT's image quality can be dramatically improved with large number of ultrasound (US) transducer elements associated multiplexed data acquisition systems, the high system cost and/or slow temporal resolution are significant problems. Here, deep learning-based approach is demonstrated that qualitatively quantitively diminishes limited-view artifacts reduce improves resolution. This learning-enhanced multiparametric dynamic volumetric PACT approach, called DL-PACT, requires only clustered subset many US on conventional PACT. Using high-quality static structural contrast-enhanced whole-body images as well functional brain live animals humans successfully acquired, all in relatively fast cost-effective manner. It believed strategy significantly advance use technology for applications such neurology, cardiology, pharmacology, endocrinology, oncology.

Language: Английский

Citations

57
Fully integrated photoacoustic microscopy and photoplethysmography of human in vivo DOI Creative Commons
Joongho Ahn, Jin Woo Baik,

Yeonggeon Kim

et al.

Photoacoustics, Journal Year: 2022, Volume and Issue: 27, P. 100374 - 100374

Published: May 20, 2022

Photoacoustic microscopy (PAM) is used to visualize blood vessels and monitor their time-dependent changes. Photoplethysmography (PPG) measures hemodynamic time-series changes such as heart rate. However, PPG's limited visual access the dynamic of has prohibited further understanding hemodynamics. Here, we propose a novel, fully integrated PAM photoplethysmography (PAM-PPG) system understand features in detail. Using PAM-PPG system, simultaneously acquire vascular images (by PAM) volume PPG) from human fingers. Next, determine rate PA signals, which match well with PPG signals. These can be measured if flow not blocked. From results, believe that could useful clinical tool various fields cardiology endocrinology.

Language: Английский

Citations

49
Bimetallic Hyaluronate-Modified Au@Pt Nanoparticles for Noninvasive Photoacoustic Imaging and Photothermal Therapy of Skin Cancer DOI
Hye Hyeon Han, Seong‐Jong Kim, Jiwoong Kim

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(9), P. 11609 - 11620

Published: Feb. 27, 2023

Although spherical gold (Au) nanoparticles have remarkable photothermal conversion efficiency and photostability, their weak absorption in the near-infrared (NIR) region poor penetration into deep tissues limited further applications to NIR light-mediated photoacoustic (PA) imaging noninvasive cancer therapy. Here, we developed bimetallic hyaluronate-modified Au-platinum (HA-Au@Pt) for theranostics by PA therapy (PTT). The growth of Pt nanodots on surface Au enhanced absorbance broadened bandwidth HA-Au@Pt plasmon resonance (SPR) coupling effect. In addition, HA facilitated transdermal delivery through skin barrier enabled clear tumor-targeted imaging. Compared conventional PTT via injection, were noninvasively delivered tumor completely ablated targeted light irradiation. Taken together, could confirm feasibility as a biophotonic agent theranostics.

Language: Английский

Citations

41
In vivo photoacoustic monitoring of vasoconstriction induced by acute hyperglycemia DOI Creative Commons
Joongho Ahn, Jin Woo Baik, Dong-Gyu Kim

et al.

Photoacoustics, Journal Year: 2023, Volume and Issue: 30, P. 100485 - 100485

Published: March 30, 2023

Postprandial hyperglycemia, blood glucose spikes, induces endothelial dysfunction, increasing cardiovascular risks. Endothelial dysfunction leads to vasoconstriction, and observation of this phenomenon is important for understanding acute hyperglycemia. However, high-resolution imaging microvessels during hyperglycemia has not been fully developed. Here, we demonstrate that photoacoustic microscopy can noninvasively monitor morphological changes in vessels live animals' extremities when rises rapidly. As level rose from 100 400 mg/dL following intraperitoneal injection, heart/breath rate, body temperature remained constant, but arterioles constricted by approximately -5.7 ± 1.1% within 20 min, gradually recovered another 40 min. In contrast, venular diameters about 0.6 1.5% arteriolar constriction. Our results experimentally statistically produces transitory vasoconstriction arterioles, with an opposite trend change glucose. These findings could help vascular homeostasis the relationship between diabetes diseases.

Language: Английский

Citations

30
Artificial intelligence-aided optical imaging for cancer theranostics DOI
Mengze Xu,

Zhiyi Chen,

Junxiao Zheng

et al.

Seminars in Cancer Biology, Journal Year: 2023, Volume and Issue: 94, P. 62 - 80

Published: June 10, 2023

Language: Английский

Citations

25
An Ultraviolet‐Transparent Ultrasound Transducer Enables High‐Resolution Label‐Free Photoacoustic Histopathology DOI
Dong-Gyu Kim, Eunwoo Park, Jeongwoo Park

et al.

Laser & Photonics Review, Journal Year: 2023, Volume and Issue: 18(2)

Published: Oct. 8, 2023

Abstract Ultraviolet photoacoustic microscopy (UV‐PAM), based on the high intrinsic optical absorption of DNA/RNA, holds great promise for intraoperative label‐free histopathological imaging modalities. Although clinical histopathology requires high‐resolution images to observe individual cell structures, conventional UV‐PAM suffers from relatively low resolution compared histological Notably, opto‐ultrasound beam combiners or ring‐shaped ultrasound transducers, which have been used as geometrically coaxial methods reflection‐mode UV‐PAMs, restrict numerical apertures (NA) due their long working distances and/or hollow structures. Here, an ultraviolet‐transparent transducer (UV‐TUT) is demonstrated that enables by improving NA (0.38) and consequent spatial (0.47 ± 0.03 µm). The study has successfully its superior performance with mouse brain tissue performed cancerous animal tissues. This work proposes ultimate form a system potential boost practical use histopathology.

Language: Английский

Citations

23
Deep learning-based virtual staining, segmentation, and classification in label-free photoacoustic histology of human specimens DOI Creative Commons
Chiho Yoon, Eunwoo Park, Sampa Misra

et al.

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Sept. 2, 2024

Language: Английский

Citations

15
3D Multiparametric Photoacoustic Computed Tomography of Primary and Metastatic Tumors in Living Mice DOI Creative Commons
Jiwoong Kim, Jihye Lee, Seongwook Choi

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(28), P. 18176 - 18190

Published: June 28, 2024

Photoacoustic computed tomography (PACT), an emerging imaging modality in preclinical cancer research, can provide multiparametric 3D information about structures, physiological functions, and pharmacokinetics. Here, we demonstrate the use of high-definition PACT both primary metastatic tumors living mice to noninvasively monitor angiogenesis, carcinogenesis, hypoxia, The system with a 1024-element hemispherical ultrasound transducer array provides isotropic spatial resolution 380 μm, effective volumetric field-of-view 12.8 mm × without scanning, acquisition time <30 s for whole mouse body. Initially, structural progression tumor microenvironment (e.g., angiogenesis vessel tortuosity) after cell inoculation. Then, analyze change oxygen saturation during verifying induced hypoxia tumor's core region. Finally, whole-body pharmacokinetics are photoacoustically imaged intravenous injection micelle-loaded IR780 dye, vivo results validated ex by fluorescence imaging. By employing premium applying analyses subcutaneous liver tumors, that this comprehensive research.

Language: Английский

Citations

13