Repeated activation of preoptic area-recipient neurons in posterior paraventricular nucleus mediates chronic heat-induced negative emotional and hyperarousal states DOI Open Access
Zhiping Cao, Wing‐Ho Yung, Ya Ke

et al.

Published: Nov. 11, 2024

Mental and behavioral disorders are associated with extended period of hot weather as found in heatwaves, but the underlying neural circuit mechanism is poorly known. The posterior paraventricular thalamus (pPVT) a hub for emotional processing receives inputs from hypothalamic preoptic area (POA), well-recognized thermoregulation center. present study was designed to explore whether chronic heat exposure leads aberrant activities POA recipient pPVT neurons subsequent changes states. By devising an air heating paradigm mimicking condition heatwaves utilizing emotion-related tests, viral track tracing, vivo calcium recordings, optogenetic manipulations electrophysiological we that 3 weeks led negative hyperarousal states mice. receive monosynaptic excitatory inhibitory innervations POA. These exhibited persistent increase activity following exposure, which essential heat-induced changes. Notably, these were also prone display stronger neuronal anxiety responses stressful situations. Furthermore, observed saturated neuroplasticity POA-pPVT pathway after occluded further potentiation. Taken together, long-term aberration offers neurobiological seen like heatwaves.

Language: Английский

The diverse role of corticotropin-releasing factor (CRF) and its CRF1 and CRF2 receptors under pathophysiological conditions: Insights into stress/anxiety, depression, and brain injury processes DOI
Helena Domin, Maria S̀miałowska

Neuroscience & Biobehavioral Reviews, Journal Year: 2024, Volume and Issue: 163, P. 105748 - 105748

Published: June 8, 2024

Language: Английский

Citations

11
Central amygdala astrocyte plasticity underlies GABAergic dysregulation in ethanol dependence DOI Creative Commons
Todd B. Nentwig, J. Daniel Obray, Anna Kruyer

et al.

Translational Psychiatry, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 8, 2025

Dependence is a hallmark of alcohol use disorder characterized by excessive intake and withdrawal symptoms. The central nucleus the amygdala (CeA) key brain structure underlying synaptic behavioral consequences ethanol dependence. While accumulating evidence suggests that astrocytes regulate transmission behavior, there limited understanding role play in present study used combination viral labeling, super resolution confocal microscopy, 3D image analysis, slice electrophysiology to determine effects chronic intermittent (CIE) exposure on astrocyte plasticity CeA. During from CIE exposure, we observed increased GABA transmission, an upregulation astrocytic GAT3 levels, proximity processes near CeA synapses. Furthermore, levels were positively associated with voluntary drinking dependent rats. Slice confirmed was functional, as unmasked GAT3-sensitive tonic current A causal for dependence assessed using viral-mediated overexpression knockdown approaches. However, or had no effect somatic symptoms, dependence-escalated intake, aversion-resistant drinking, post-dependent male female Moreover, intra-CeA pharmacological inhibition did not alter drinking. Together, these findings indicate induces GABAergic dysregulation changes do appear be necessary related phenotypes

Language: Английский

Citations

1
The link between early-life adversity and later alcohol use disorder: a role for microglia? DOI Creative Commons

H Lichtenstein,

Michelle K. Sequeira, Jessica L. Bolton

et al.

Neurobiology of Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100714 - 100714

Published: Feb. 1, 2025

Language: Английский

Citations

0
Repeated activation of preoptic area-recipient neurons in posterior paraventricular nucleus mediates chronic heat-induced negative emotional valence and hyperarousal states DOI Open Access
Zhiping Cao, Wing‐Ho Yung, Ya Ke

et al.

Published: March 5, 2025

Mental and behavioral disorders are associated with extended period of hot weather as found in heatwaves, but the underlying neural circuit mechanism is poorly known. The posterior paraventricular thalamus (pPVT) a hub for emotional processing receives inputs from hypothalamic preoptic area (POA), well-recognized thermoregulation center. present study was designed to explore whether chronic heat exposure leads aberrant activities POA recipient pPVT neurons subsequent changes states. By devising an air heating paradigm mimicking condition heatwaves utilizing emotion-related tests, viral track tracing, vivo calcium recordings, optogenetic manipulations electrophysiological we that 3 weeks led negative valence hyperarousal states mice. receive monosynaptic excitatory inhibitory innervations POA. These exhibited persistent increase activity following exposure, which essential heat-induced changes. Notably, these were also prone display stronger neuronal anxiety responses stressful situations. Furthermore, observed saturated neuroplasticity POA-pPVT pathway after occluded further potentiation. Taken together, long-term aberration offers neurobiological seen periods like heatwaves.

Language: Английский

Citations

0
Repeated activation of preoptic area recipient neurons in posterior paraventricular nucleus mediates chronic heat-induced negative emotional valence and hyperarousal states DOI Creative Commons
Zhiping Cao, Wing‐Ho Yung, Ya Ke

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: April 9, 2025

Mental and behavioral disorders are associated with extended period of hot weather as found in heatwaves, but the underlying neural circuit mechanism remains poorly known. The posterior paraventricular thalamus (pPVT) is a hub for emotional processing receives inputs from hypothalamic preoptic area (POA), well-recognized thermoregulation center. present study was designed to explore whether chronic heat exposure leads aberrant activities POA recipient pPVT neurons subsequent changes states. By devising an air heating paradigm mimicking condition heatwaves utilizing emotion-related tests, viral tract tracing, vivo calcium recordings, optogenetic manipulations, electrophysiological we that 3 weeks led negative valence hyperarousal states mice. receive monosynaptic excitatory inhibitory innervations POA. These exhibited persistent increase activity following exposure, which essential heat-induced changes. Notably, these were also prone display stronger neuronal anxiety responses stressful situations. Furthermore, observed saturated neuroplasticity POA-pPVT pathway after occluded further potentiation. Taken together, long-term aberration offers neurobiological seen periods like heatwaves.

Language: Английский

Citations

0
Corticotropin-Releasing Hormone Neurons in the Central Amygdala Nucleus: An Integrative Hub for Pain, Emotion, and Addiction Neurobiology DOI
Jia Li, Ziang Li, Haifeng Tian

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Apremilast reduces co-occurring alcohol drinking and mechanical allodynia and regulates central amygdala GABAergic transmission DOI Creative Commons
Valentina Vozella, Vittoria Borgonetti, Bryan Cruz

et al.

JCI Insight, Journal Year: 2025, Volume and Issue: 10(8)

Published: April 21, 2025

The FDA-approved phosphodiesterase type 4 (PDE4) inhibitor, apremilast, has been recently investigated as a pharmacotherapy for alcohol use disorder (AUD) with promising efficacy in rodent models and humans. However, apremilast's effects on mechanical allodynia associated AUD well distinct responses of this drug between males females are understudied. present study examined the behavioral electrophysiological apremilast Marchigian Sardinian alcohol-preferring (msP) rats their Wistar counterparts. We used 2-bottle choice (2-BC) drinking procedure tested sensitivity across our regimen. Spontaneous inhibitory GABA-mediated postsynaptic currents from central nucleus amygdala (CeA) following application were subset using ex vivo electrophysiology. Transcript levels Pde4a or -4b subtypes assessed modulation by alcohol. Apremilast reduced both strains rats. immediately after drinking, persisting into early late abstinence. increased GABAergic transmission CeA slices alcohol-exposed Wistars but not msP rats, suggesting neuroadaptations msPs excessive allodynia. Pde4 subtype transcript These results suggest that alleviates co-occurring pain sensitivity, they further confirm PDE4's role pain-associated AUD.

Language: Английский

Citations

0
Predator odor stress produces sex- and subpopulation-specific increases in alcohol drinking, anxiety-like behavior, and lateral hypothalamic Crh expression DOI

Sydney M. Bonauto,

Olivia R Brunke,

Fair M. Vassoler

et al.

Neuropharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 110511 - 110511

Published: May 1, 2025

Language: Английский

Citations

0
Corticotropin-releasing hormone neurons in the central Amygdala: An integrative hub for pain, emotion, and addiction neurobiology DOI
Jia Li, Ziang Li, Haifeng Tian

et al.

Brain Research, Journal Year: 2025, Volume and Issue: unknown, P. 149753 - 149753

Published: June 1, 2025

Language: Английский

Citations

0
Repeated activation of preoptic area-recipient neurons in posterior paraventricular nucleus mediates chronic heat-induced negative emotional and hyperarousal states DOI Open Access
Zhiping Cao, Wing‐Ho Yung, Ya Ke

et al.

Published: Nov. 11, 2024

Mental and behavioral disorders are associated with extended period of hot weather as found in heatwaves, but the underlying neural circuit mechanism is poorly known. The posterior paraventricular thalamus (pPVT) a hub for emotional processing receives inputs from hypothalamic preoptic area (POA), well-recognized thermoregulation center. present study was designed to explore whether chronic heat exposure leads aberrant activities POA recipient pPVT neurons subsequent changes states. By devising an air heating paradigm mimicking condition heatwaves utilizing emotion-related tests, viral track tracing, vivo calcium recordings, optogenetic manipulations electrophysiological we that 3 weeks led negative valence hyperarousal states mice. receive monosynaptic excitatory inhibitory innervations POA. These exhibited persistent increase activity following exposure, which essential heat-induced changes. Notably, these were also prone display stronger neuronal anxiety responses stressful situations. Furthermore, observed saturated neuroplasticity POA-pPVT pathway after occluded further potentiation. Taken together, long-term aberration offers neurobiological seen periods like heatwaves.

Language: Английский

Citations

1