Dynamic Expression of Genes Encoding Ubiquitin Conjugating Enzymes (E2s) During Neuronal Differentiation and Maturation: Implications for Neurodevelopmental Disorders and Neurodegenerative Diseases DOI Open Access

Agathe Paubel,

Sylviane Marouillat, Audrey Dangoumau

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(11), P. 1381 - 1381

Published: Oct. 26, 2024

Background: The ubiquitination process plays a crucial role in neuronal differentiation and function. Numerous studies have focused on the expression functions of E3 ligases during these different stages, far fewer E2 conjugating enzymes. In mice, as humans, E2s belong to 17 enzyme families. Objectives: We analyzed by real-time PCR dynamics all known genes an vitro mouse hippocampal cultures, after, we their stimulation with N-methyl-D-aspartate (NMDA). Results: found that 36 38 were expressed neurons. Many up-regulated neuritogenesis and/or synaptogenesis such Ube2h, Ube2b, Aktip. Rapid delayed responses NMDA associated increased several genes, Ube2i, SUMO-conjugating enzyme. also observed similar profiles within same gene family, consistent presence transcription factor binding sites respective promoter sequences. Conclusions: Our study indicates specific are correlated changes activity. A better understanding regulation function is needed understand played physiological mechanisms pathophysiological alterations involved neurodevelopmental or neurodegenerative diseases.

Language: Английский

Lancao decoction alleviates Alzheimer’s disease: depending on activating CaMKII to protect neuronal refunction by reducing β-amyloid in the hippocampus DOI
Lei Wu, Yan Sun, Lingang Zhao

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119619 - 119619

Published: March 1, 2025

Language: Английский

Citations

1
The Histone Lysine Demethylase KDM7A Contributes to Reward Memory via Fscn1‐Induced Synaptic Plasticity in the Medial Prefrontal Cortex DOI Creative Commons

Zhuojin Yang,

Dongyu Yu, Feifei Gao

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract Lysine demethylase 7A (KDM7A) catalyzes the removal of dimethylation from histone H3 lysine 9 and 27, both which are associated with transcription repression. Previous study indicates that Kdm7a mRNA in medial prefrontal cortex (mPFC) increases after drug exposure, yet its role drug‐related behaviors is largely unknown. In a morphine‐conditioned place preference (CPP) paradigm, these findings reveal specific increase expression mPFC 7 days withdrawal. Subsequently, results demonstrate knockdown do not affect acquisition morphine‐induced CPP, but it attenuate memory consolidation. To further explore Kdm7a‐mediated transcriptomic changes, this work employs Nanopore direct RNA sequencing. Transcriptome profiling unveils several gene alterations impacted by KDM7A, enriched relevant neural function categories. Notably, identifies validates fascin actin‐bundling protein 1 (Fscn1) as downstream molecular target. Knockdown Fscn1 has similar impact on CPP to Kdm7a, along corresponding decrease dendritic spine density neuronal activity mPFC. Additionally, silencing decreases enrichment H3K9me2 H3K27me2 at promoter region, suggesting KDM7A may act crucial regulator transcriptional responses morphine‐related reward via Fscn1.

Language: Английский

Citations

0
Somatostatin-expressing interneurons of prefrontal cortex modulate social deficits in the Magel2 mouse model of autism DOI Creative Commons

Xiaona Wang,

Mengyuan Chen,

Daoqi Mei

et al.

Molecular Autism, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 11, 2025

Dysfunction in social interactions is a core symptom of autism spectrum disorder (ASD). Nevertheless, the neural mechanisms underlying deficits ASD are poorly understood. By integrating electrophysiological, vivo fiber photometry, viral-mediated tracing, optogenetic and pharmacological stimulation, we show reduced intrinsic excitability hypoactivity SOM interneurons medial prefrontal cortex (mPFC) Magel2-deficient mice, an established model, were required to defects. Chemogenetic inhibition mPFC SOM-containing resulted interaction wild-type Magel2 mice. These sociability can be rescued by activation mPFCSOM-LS inhibitory pathway Magel 2 knockout results demonstrate for action impairments, suggest targeting this mechanism that may prove therapeutically beneficial mitigating behavioral disturbances observed ASD.

Language: Английский

Citations

0
Dynamic Expression of Genes Encoding Ubiquitin Conjugating Enzymes (E2s) During Neuronal Differentiation and Maturation: Implications for Neurodevelopmental Disorders and Neurodegenerative Diseases DOI Open Access

Agathe Paubel,

Sylviane Marouillat, Audrey Dangoumau

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(11), P. 1381 - 1381

Published: Oct. 26, 2024

Background: The ubiquitination process plays a crucial role in neuronal differentiation and function. Numerous studies have focused on the expression functions of E3 ligases during these different stages, far fewer E2 conjugating enzymes. In mice, as humans, E2s belong to 17 enzyme families. Objectives: We analyzed by real-time PCR dynamics all known genes an vitro mouse hippocampal cultures, after, we their stimulation with N-methyl-D-aspartate (NMDA). Results: found that 36 38 were expressed neurons. Many up-regulated neuritogenesis and/or synaptogenesis such Ube2h, Ube2b, Aktip. Rapid delayed responses NMDA associated increased several genes, Ube2i, SUMO-conjugating enzyme. also observed similar profiles within same gene family, consistent presence transcription factor binding sites respective promoter sequences. Conclusions: Our study indicates specific are correlated changes activity. A better understanding regulation function is needed understand played physiological mechanisms pathophysiological alterations involved neurodevelopmental or neurodegenerative diseases.

Language: Английский

Citations

0