Changes in RNA Splicing: A New Paradigm of Transcriptional Responses to Probiotic Action in the Mammalian Brain

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 165 - 165

Published: Jan. 14, 2025

Elucidating the gene regulatory mechanisms underlying gut–brain axis is critical for uncovering novel interaction pathways and developing therapeutic strategies gut bacteria-associated neurological disorders. Most studies have primarily investigated how bacteria modulate host epigenetics expression; their impact on alternative splicing, particularly in brain, remains largely unexplored. Here, we effects of gut-associated probiotic Lacidofil® splicing across 10 regions rat brain using published RNA-sequencing data. The altogether altered 2941 differential events, predominantly, skipped exon (SE) mutually exclusive (MXE) events. Protein–protein interactions a KEGG analysis differentially spliced genes (DSGs) revealed consistent enrichment spliceosome vesicle transport complexes, as well related to neurodegenerative diseases, synaptic function plasticity, substance addiction regions. Using PsyGeNET platform, found that DSGs from locus coeruleus (LConly), medial preoptic area (mPOA), ventral dentate gyrus (venDG) were enriched depression-associated or schizophrenia-associated genes. Notably, highlight App gene, where precisely regulated two exons causally involved amyloid β protein-based diseases. Although factors exhibited both plasticity expression response Lacidofil®, overlap between expressed (DEGs) most was rather low. Our study provides mechanistic insight into probiotics might influence through modulation RNA splicing.

Language: Английский

A PRISMA Systematic Review of Sexual Dysfunction and Probiotics with Pathophysiological Mechanisms

Biology, Journal Year: 2025, Volume and Issue: 14(3), P. 286 - 286

Published: March 11, 2025

Sexual dysfunction, influenced by hormonal imbalances, psychological factors, and chronic diseases, affects a significant portion of the population. Probiotics, known for their beneficial effects on gut microbiota, have emerged as potential therapeutic agents improving sexual health. This systematic review evaluates impact probiotics function, regulation, reproductive outcomes. A comprehensive search identified 3308 studies, with 12 meeting inclusion criteria—comprising 10 randomized controlled trials (RCTs) 2 in vivo vitro studies. Probiotic interventions were shown to significantly improve particularly women undergoing antidepressant therapy (p < 0.05). Significant improvements Female Function Index (FSFI) scores observed, combined treatments such Lactofem Letrozole selective serotonin reuptake inhibitors (SSRIs) demonstrating 10% biochemical clinical pregnancy rate compared 0% control group = use was also associated 66% reduction menopausal symptoms, increased sperm motility (36.08%), viability (46.79%), morphology (36.47%). Probiotics contributed favorable changes, including reduced luteinizing hormone (LH) follicle-stimulating (FSH) ratio (from 3.0 2.5, p 0.05) testosterone levels. Regarding outcomes, probiotic higher rates fertility motility, viability, men. highlights promising role addressing dysfunction health, suggesting adjunctive conditions depression infertility. Further research is needed better understand underlying mechanisms these effects.

Language: Английский

Genetic and molecular basis of abnormal BOLD signaling variability in patients with major depressive disorder after electroconvulsive therapy

Translational Psychiatry, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 2, 2025

Abstract Electroconvulsive therapy (ECT) is an effective and rapid neuromodulatory intervention for treatment-resistant major depressive disorders (MDD). However, the precise mechanisms underlying their efficacies remain unclear. Resting-state functional magnetic resonance imaging (fMRI) data were collected from 84 individuals with MDD healthy controls before after ECT, coefficient of variation BOLD signal (CVBOLD) analysis was combined region interest (ROI) connectivity (FC) analysis. To assess reliability antidepressant mechanism we analyzed changes in CVBOLD a separate cohort consisting 35 patients who underwent ECT. Moreover, transcriptomic neurotransmitter receptor used to reveal genetic molecular bases CVBOLD. Patients ECT demonstrated increased left angular cortex precuneus. Following increase FC between precuneus right lingual lobes associated improvements Hamilton Depression Rating Scale (HAMD) scores. validation consistently similar two independent cohorts MDD. these closely thyroid hormone synthesis, oxidative phosphorylation, endocytosis, insulin signaling pathway, significantly correlated receptor/transporter density serotonin dopamine. These findings suggest that modulates abnormal functions precuneus, leading widespread neuroplasticity, especially default mode network, exerts effect.

Language: Английский