Resistance of Pseudomonas aeruginosa to Antibiotics During Long-Term Persistence in Patients with Cystic Fibrosis DOI Creative Commons
N. L. Belkova, У. М. Немченко, E. S. Klimenko

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 302 - 302

Published: March 14, 2025

Pseudomonas aeruginosa is one of the leading causes nosocomial respiratory tract infections, significantly affecting morbidity and mortality. It can persist in lungs patients with cystic fibrosis (CF) for extended periods because its adaptive capacity. The main aim this study was to determine phenotypic genotypic resistance antibiotics clinical isolates P. that CF receiving long-term antimicrobial therapy. included nine strains isolated from sputum admitted hospital. Susceptibility determined using European Committee on Antimicrobial Testing (EUCAST) criteria. Whole-genome sequencing performed phylogeny, sequence typing, identify antibiotic-resistant genes. showed during persistence antibacterial therapy, restoration susceptibility occurred some cases. Multilocus typing phylogeny revealed six types. Functional annotation identified 72 genes responsible chemical substances, either chromosomal or plasmid localisation.

Language: Английский

The role of bacterial metabolism in antimicrobial resistance DOI
Mehrose Ahmad, Sai Varun Aduru, Robert P. Smith

et al.

Nature Reviews Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Language: Английский

Citations

6

Tuberculosis vaccines and therapeutic drug: challenges and future directions DOI Creative Commons

Yajing An,

Ruizi Ni,

Zhuang Li

et al.

Molecular Biomedicine, Journal Year: 2025, Volume and Issue: 6(1)

Published: Jan. 22, 2025

Abstract Tuberculosis (TB) remains a prominent global health challenge, with the World Health Organization documenting over 1 million annual fatalities. Despite deployment of Bacille Calmette-Guérin (BCG) vaccine and available therapeutic agents, escalation drug-resistant Mycobacterium tuberculosis strains underscores pressing need for more efficacious vaccines treatments. This review meticulously maps out contemporary landscape TB development, focus on antigen identification, clinical trial progress, obstacles future trajectories in research. We spotlight innovative approaches, such as multi-antigen mRNA technology platforms. Furthermore, delves into current therapeutics, particularly multidrug-resistant (MDR-TB), exploring promising agents like bedaquiline (BDQ) delamanid (DLM), well potential host-directed therapies. The hurdles development encompass overcoming diversity, enhancing effectiveness across diverse populations, advancing novel Future initiatives emphasize combinatorial strategies, anti-TB compounds targeting pathways, personalized medicine treatment prevention. notable advances, persistent challenges diagnostic failures protracted regimens continue to impede progress. work aims steer research endeavors toward groundbreaking providing crucial insights prevention strategies.

Language: Английский

Citations

4

Metal‐Protein Hybrid Materials: Unlocking New Frontiers in Biomedical Applications DOI Open Access
Yong Pan, Han Zhao, Wenyong Huang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract Metal‐protein hybrid materials represent a novel class of functional that exhibit exceptional physicochemical properties and tunable structures, rendering them remarkable applications in diverse fields, including engineering, biocatalysis, biosensing, biomedicine. The design development multifunctional biocompatible metal‐protein have been the subject extensive research key aspiration for practical clinical settings. This review provides comprehensive analysis strategies, intrinsic properties, biomedical these materials, with specific emphasis on their potential cancer therapy, drug vaccine delivery, antibacterial treatments, tissue regeneration. Through rational design, stable can be synthesized using straightforward methods, enabling therapeutic, immunomodulatory, other desired functionalities. Finally, outlines existing limitations challenges associated evaluates translation, providing insights into implementation within applications.

Language: Английский

Citations

3

Emerging models to study competitive interactions within bacterial communities DOI Creative Commons
Mollie Virgo, Serge Mostowy, Brian T. Ho

et al.

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Within both abiotic and host environments, bacteria typically exist as diverse, multispecies communities have crucial roles in human health, agriculture, industry. In these communities, compete for resources, competitive interactions can shape the overall population structure community function. Studying bacterial dynamics requires experimental model systems that capture different interaction networks between their surroundings. We examine recent literature advancing such systems, including (i) silico models establishing theoretical basis how cell-to-cell influence level dynamics, (ii) vitro characterizing specific interbacterial interactions, (iii) organ-on-a-chip revealing physiologically relevant parameters, spatial mechanical forces, encounter within a host, (iv) vivo plant animal connecting responses to interactions. Each of has greatly contributed our understanding be used synergistically understand competition influences architecture.

Language: Английский

Citations

1

Designing Effective Antimicrobial Agents: Structural Insights into the Antibiofilm Activity of Ionic Liquids DOI

Alexey D. Tikhomirov,

Ksenia S. Egorova, Valentine P. Ananikov

et al.

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Research concerning biofilm control is critical due to the pervasive and resilient nature of biofilms, which pose significant challenges across industrial, environmental, healthcare sectors. Traditional antimicrobial treatments are often ineffective against these robust structures. Here, we explore properties ionic liquids (ILs) their efficacy in disruption. By examining structural variations ILs, highlight key role hydrophobicity, noting that longer alkyl side chains IL cations enhance disruption bacterial death. However, upon reaching a certain optimal chain length─usually C12 C14─the activity ILs starts decrease. Furthermore, symmetry size anions significantly impact elimination. This Perspective addresses gap research, revealing structure–activity relationships providing foundation for designing more effective biofilm-disrupting agents.

Language: Английский

Citations

1

Antibacterial and anti-biofilm activities of Derazantinib (ARQ-087) against Staphylococcus aureus DOI
Weiguo Wang, Q.P. Zhong,

Xincheng Huang

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(4)

Published: March 6, 2025

Language: Английский

Citations

1

Biocides as Drivers of Antibiotic Resistance: A Critical Review of Environmental Implications and Public Health Risks DOI Creative Commons
Mariana Sousa, Idalina Machado, Lúcia C. Simões

et al.

Environmental Science and Ecotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 100557 - 100557

Published: March 1, 2025

The widespread and indiscriminate use of biocides poses significant threats to global health, socioeconomic development, environmental sustainability by accelerating antibiotic resistance. Bacterial resistance development is highly complex influenced significantly factors. Increased biocide usage in households, agriculture, livestock farming, industrial settings, hospitals produces persistent chemical residues that pollute soil aquatic environments. Such contaminants contribute the selection proliferation resistant bacteria antimicrobial genes (ARGs), facilitating their dissemination among humans, animals, ecosystems. In this review, we conduct a critical assessment four issues pertaining topic. Specifically, (i) role exerting selective pressure within resistome, thereby promoting microbial populations contributing spread (ARGs); (ii) triggering transient phenotypic adaptations bacteria, including efflux pump overexpression, membrane alterations, reduced porin expression, which often result cross-resistance multiple antibiotics; (iii) capacity disrupt make genetic content accessible, releasing DNA into environment remains intact under certain conditions, horizontal gene transfer determinants; (iv) bacterial cells, enhancing interactions between biofilms environment, strengthening biofilm cohesion, inducing mechanisms, creating reservoirs for microorganisms ARG dissemination. Collectively, review highlights public health implications use, emphasizing an urgent need strategic interventions mitigate proliferation.

Language: Английский

Citations

1

2-hydroxylbenzoyl-pyrazolo[1,5-a]pyrimidines as inhibitors against bacterial pathogens and MCF7 human breast cancer cells DOI
Heba Hassan, Abdul Qaiyum Ramle, Chun Hoe Tan

et al.

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142278 - 142278

Published: April 1, 2025

Language: Английский

Citations

1

Discovery of berberine derivatives@chitosan nanoparticles for controlling plant canker diseases DOI
Mei Zhu, Danping Chen, Cheng‐Peng Li

et al.

Food Hydrocolloids, Journal Year: 2025, Volume and Issue: unknown, P. 111054 - 111054

Published: Jan. 1, 2025

Language: Английский

Citations

0

Identification of 6,8-Ditrifluoromethyl Halogenated Phenazine as a Potent Bacterial Biofilm-Eradicating Agent DOI Creative Commons
Qiwen Gao, Hongfen Yang, Jeremy Sheiber

et al.

Organic & Biomolecular Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Bacterial biofilms are surface-attached communities consisting of non-replicating persister cells encased within an extracellular matrix biomolecules. Unlike bacteria that have acquired resistance to antibiotics, enable demonstrate innate tolerance toward all classes conventional antibiotic therapies. It is estimated 50-80% bacterial infections biofilm associated, which considered the underlying cause chronic and recurring infections. Herein, we report a modular three-step synthetic route new halogenated phenazine (HP) analogues from diverse aniline nitroarene building blocks. The HPs were evaluated for antibacterial biofilm-killing properties against panel lab strains multidrug-resistant clinical isolates. Several demonstrated potent (MIC ≤ 0.39 μM) biofilm-eradicating activities (MBEC < 10 with 6,8-ditrifluoromethyl-HP 15 remarkable potencies = 0.15-1.17 Gram-positive pathogens, including methicillin-resistant Staphylococcus aureus Confocal microscopy showed HP induced significant losses in polysaccharide MRSA biofilms. In addition, increased dormant Mycobacterium tuberculosis (Mtb, MIC 1.35 when compared replicating Mtb 3.69 μM). Overall, this has enabled rapid access interesting series explore their unique properties.

Language: Английский

Citations

0