Antibiotics,
Journal Year:
2025,
Volume and Issue:
14(3), P. 302 - 302
Published: March 14, 2025
Pseudomonas
aeruginosa
is
one
of
the
leading
causes
nosocomial
respiratory
tract
infections,
significantly
affecting
morbidity
and
mortality.
It
can
persist
in
lungs
patients
with
cystic
fibrosis
(CF)
for
extended
periods
because
its
adaptive
capacity.
The
main
aim
this
study
was
to
determine
phenotypic
genotypic
resistance
antibiotics
clinical
isolates
P.
that
CF
receiving
long-term
antimicrobial
therapy.
included
nine
strains
isolated
from
sputum
admitted
hospital.
Susceptibility
determined
using
European
Committee
on
Antimicrobial
Testing
(EUCAST)
criteria.
Whole-genome
sequencing
performed
phylogeny,
sequence
typing,
identify
antibiotic-resistant
genes.
showed
during
persistence
antibacterial
therapy,
restoration
susceptibility
occurred
some
cases.
Multilocus
typing
phylogeny
revealed
six
types.
Functional
annotation
identified
72
genes
responsible
chemical
substances,
either
chromosomal
or
plasmid
localisation.
Molecular Biomedicine,
Journal Year:
2025,
Volume and Issue:
6(1)
Published: Jan. 22, 2025
Abstract
Tuberculosis
(TB)
remains
a
prominent
global
health
challenge,
with
the
World
Health
Organization
documenting
over
1
million
annual
fatalities.
Despite
deployment
of
Bacille
Calmette-Guérin
(BCG)
vaccine
and
available
therapeutic
agents,
escalation
drug-resistant
Mycobacterium
tuberculosis
strains
underscores
pressing
need
for
more
efficacious
vaccines
treatments.
This
review
meticulously
maps
out
contemporary
landscape
TB
development,
focus
on
antigen
identification,
clinical
trial
progress,
obstacles
future
trajectories
in
research.
We
spotlight
innovative
approaches,
such
as
multi-antigen
mRNA
technology
platforms.
Furthermore,
delves
into
current
therapeutics,
particularly
multidrug-resistant
(MDR-TB),
exploring
promising
agents
like
bedaquiline
(BDQ)
delamanid
(DLM),
well
potential
host-directed
therapies.
The
hurdles
development
encompass
overcoming
diversity,
enhancing
effectiveness
across
diverse
populations,
advancing
novel
Future
initiatives
emphasize
combinatorial
strategies,
anti-TB
compounds
targeting
pathways,
personalized
medicine
treatment
prevention.
notable
advances,
persistent
challenges
diagnostic
failures
protracted
regimens
continue
to
impede
progress.
work
aims
steer
research
endeavors
toward
groundbreaking
providing
crucial
insights
prevention
strategies.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 7, 2025
Abstract
Metal‐protein
hybrid
materials
represent
a
novel
class
of
functional
that
exhibit
exceptional
physicochemical
properties
and
tunable
structures,
rendering
them
remarkable
applications
in
diverse
fields,
including
engineering,
biocatalysis,
biosensing,
biomedicine.
The
design
development
multifunctional
biocompatible
metal‐protein
have
been
the
subject
extensive
research
key
aspiration
for
practical
clinical
settings.
This
review
provides
comprehensive
analysis
strategies,
intrinsic
properties,
biomedical
these
materials,
with
specific
emphasis
on
their
potential
cancer
therapy,
drug
vaccine
delivery,
antibacterial
treatments,
tissue
regeneration.
Through
rational
design,
stable
can
be
synthesized
using
straightforward
methods,
enabling
therapeutic,
immunomodulatory,
other
desired
functionalities.
Finally,
outlines
existing
limitations
challenges
associated
evaluates
translation,
providing
insights
into
implementation
within
applications.
Trends in Microbiology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Within
both
abiotic
and
host
environments,
bacteria
typically
exist
as
diverse,
multispecies
communities
have
crucial
roles
in
human
health,
agriculture,
industry.
In
these
communities,
compete
for
resources,
competitive
interactions
can
shape
the
overall
population
structure
community
function.
Studying
bacterial
dynamics
requires
experimental
model
systems
that
capture
different
interaction
networks
between
their
surroundings.
We
examine
recent
literature
advancing
such
systems,
including
(i)
silico
models
establishing
theoretical
basis
how
cell-to-cell
influence
level
dynamics,
(ii)
vitro
characterizing
specific
interbacterial
interactions,
(iii)
organ-on-a-chip
revealing
physiologically
relevant
parameters,
spatial
mechanical
forces,
encounter
within
a
host,
(iv)
vivo
plant
animal
connecting
responses
to
interactions.
Each
of
has
greatly
contributed
our
understanding
be
used
synergistically
understand
competition
influences
architecture.
Journal of Medicinal Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 3, 2025
Research
concerning
biofilm
control
is
critical
due
to
the
pervasive
and
resilient
nature
of
biofilms,
which
pose
significant
challenges
across
industrial,
environmental,
healthcare
sectors.
Traditional
antimicrobial
treatments
are
often
ineffective
against
these
robust
structures.
Here,
we
explore
properties
ionic
liquids
(ILs)
their
efficacy
in
disruption.
By
examining
structural
variations
ILs,
highlight
key
role
hydrophobicity,
noting
that
longer
alkyl
side
chains
IL
cations
enhance
disruption
bacterial
death.
However,
upon
reaching
a
certain
optimal
chain
length─usually
C12
C14─the
activity
ILs
starts
decrease.
Furthermore,
symmetry
size
anions
significantly
impact
elimination.
This
Perspective
addresses
gap
research,
revealing
structure–activity
relationships
providing
foundation
for
designing
more
effective
biofilm-disrupting
agents.
Environmental Science and Ecotechnology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 100557 - 100557
Published: March 1, 2025
The
widespread
and
indiscriminate
use
of
biocides
poses
significant
threats
to
global
health,
socioeconomic
development,
environmental
sustainability
by
accelerating
antibiotic
resistance.
Bacterial
resistance
development
is
highly
complex
influenced
significantly
factors.
Increased
biocide
usage
in
households,
agriculture,
livestock
farming,
industrial
settings,
hospitals
produces
persistent
chemical
residues
that
pollute
soil
aquatic
environments.
Such
contaminants
contribute
the
selection
proliferation
resistant
bacteria
antimicrobial
genes
(ARGs),
facilitating
their
dissemination
among
humans,
animals,
ecosystems.
In
this
review,
we
conduct
a
critical
assessment
four
issues
pertaining
topic.
Specifically,
(i)
role
exerting
selective
pressure
within
resistome,
thereby
promoting
microbial
populations
contributing
spread
(ARGs);
(ii)
triggering
transient
phenotypic
adaptations
bacteria,
including
efflux
pump
overexpression,
membrane
alterations,
reduced
porin
expression,
which
often
result
cross-resistance
multiple
antibiotics;
(iii)
capacity
disrupt
make
genetic
content
accessible,
releasing
DNA
into
environment
remains
intact
under
certain
conditions,
horizontal
gene
transfer
determinants;
(iv)
bacterial
cells,
enhancing
interactions
between
biofilms
environment,
strengthening
biofilm
cohesion,
inducing
mechanisms,
creating
reservoirs
for
microorganisms
ARG
dissemination.
Collectively,
review
highlights
public
health
implications
use,
emphasizing
an
urgent
need
strategic
interventions
mitigate
proliferation.
Bacterial
biofilms
are
surface-attached
communities
consisting
of
non-replicating
persister
cells
encased
within
an
extracellular
matrix
biomolecules.
Unlike
bacteria
that
have
acquired
resistance
to
antibiotics,
enable
demonstrate
innate
tolerance
toward
all
classes
conventional
antibiotic
therapies.
It
is
estimated
50-80%
bacterial
infections
biofilm
associated,
which
considered
the
underlying
cause
chronic
and
recurring
infections.
Herein,
we
report
a
modular
three-step
synthetic
route
new
halogenated
phenazine
(HP)
analogues
from
diverse
aniline
nitroarene
building
blocks.
The
HPs
were
evaluated
for
antibacterial
biofilm-killing
properties
against
panel
lab
strains
multidrug-resistant
clinical
isolates.
Several
demonstrated
potent
(MIC
≤
0.39
μM)
biofilm-eradicating
activities
(MBEC
<
10
with
6,8-ditrifluoromethyl-HP
15
remarkable
potencies
=
0.15-1.17
Gram-positive
pathogens,
including
methicillin-resistant
Staphylococcus
aureus
Confocal
microscopy
showed
HP
induced
significant
losses
in
polysaccharide
MRSA
biofilms.
In
addition,
increased
dormant
Mycobacterium
tuberculosis
(Mtb,
MIC
1.35
when
compared
replicating
Mtb
3.69
μM).
Overall,
this
has
enabled
rapid
access
interesting
series
explore
their
unique
properties.
