Does anticancer therapy directly contribute to antimicrobial resistance?

The Lancet Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 101115 - 101115

Published: March 1, 2025

Language: Английский

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Heavy metals promote the formation of multidrug-tolerant Staphylococcus aureus and Escherichia coli persisters

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 293, P. 118014 - 118014

Published: March 1, 2025

Bacterial persisters are dormant phenotypic variants that tolerant to antibiotics, contributing treatment failure and the emergence of antimicrobial resistance. Although formation has been extensively studied in regards bacterial infections treatment, such as antibiotic exposure or intracellular survival within macrophages, role environmental stressors persister remains largely unexplored. In this study, we investigate heavy metals, specifically arsenic (As), cadmium (Cd), mercury (Hg), promoting cell Staphylococcus aureus Escherichia coli. Log-phase cultures were exposed metals (5 mM As, 1.25 Cd, 4 µM Hg for S. aureus; 12.5 2 15 E. coli) 0.5 h induce cells. We observed these induced formation, confirmed by ATP levels through microscopy luciferase assays, well reactive oxygen species (ROS) using carboxy-H2DCFDA. Short-term metal strongly depleted while generating ROS. Moreover, enhanced expression genes involved SOS response, including recA, umuC, dinB, rexA, rexB, sulA, rpoS, soxR, measured qPCR. This response was likely elevated ROS following exposure. Furthermore, demonstrate metal-induced exhibited a substantially increased resistance, shown ciprofloxacin resistance developing presence metals. Therefore, our results clearly can cells depleting cellular ROS, responses contribute These findings highlight intricate relationship between emphasizing need "One Health" strategy address growing crisis.

Language: Английский

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Resistance of Pseudomonas aeruginosa to Antibiotics During Long-Term Persistence in Patients with Cystic Fibrosis

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 302 - 302

Published: March 14, 2025

Pseudomonas aeruginosa is one of the leading causes nosocomial respiratory tract infections, significantly affecting morbidity and mortality. It can persist in lungs patients with cystic fibrosis (CF) for extended periods because its adaptive capacity. The main aim this study was to determine phenotypic genotypic resistance antibiotics clinical isolates P. that CF receiving long-term antimicrobial therapy. included nine strains isolated from sputum admitted hospital. Susceptibility determined using European Committee on Antimicrobial Testing (EUCAST) criteria. Whole-genome sequencing performed phylogeny, sequence typing, identify antibiotic-resistant genes. showed during persistence antibacterial therapy, restoration susceptibility occurred some cases. Multilocus typing phylogeny revealed six types. Functional annotation identified 72 genes responsible chemical substances, either chromosomal or plasmid localisation.

Language: Английский

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Biocides as Drivers of Antibiotic Resistance: A Critical Review of Environmental Implications and Public Health Risks

Environmental Science and Ecotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 100557 - 100557

Published: March 1, 2025

The widespread and indiscriminate use of biocides poses significant threats to global health, socioeconomic development, environmental sustainability by accelerating antibiotic resistance. Bacterial resistance development is highly complex influenced significantly factors. Increased biocide usage in households, agriculture, livestock farming, industrial settings, hospitals produces persistent chemical residues that pollute soil aquatic environments. Such contaminants contribute the selection proliferation resistant bacteria antimicrobial genes (ARGs), facilitating their dissemination among humans, animals, ecosystems. In this review, we conduct a critical assessment four issues pertaining topic. Specifically, (i) role exerting selective pressure within resistome, thereby promoting microbial populations contributing spread (ARGs); (ii) triggering transient phenotypic adaptations bacteria, including efflux pump overexpression, membrane alterations, reduced porin expression, which often result cross-resistance multiple antibiotics; (iii) capacity disrupt make genetic content accessible, releasing DNA into environment remains intact under certain conditions, horizontal gene transfer determinants; (iv) bacterial cells, enhancing interactions between biofilms environment, strengthening biofilm cohesion, inducing mechanisms, creating reservoirs for microorganisms ARG dissemination. Collectively, review highlights public health implications use, emphasizing an urgent need strategic interventions mitigate proliferation.

Language: Английский

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2-hydroxylbenzoyl-pyrazolo[1,5-a]pyrimidines as inhibitors against bacterial pathogens and MCF7 human breast cancer cells

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142278 - 142278

Published: April 1, 2025

Language: Английский

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