Langmuir,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 11, 2025
Cell
adhesion
is
a
fundamental
necessity
for
anchorage-dependent
cells
to
thrive
in
the
matrix.
This
process
serves
as
initial
stage
sequence
of
cellular
activities,
which
includes
cell
diffusion,
migration,
proliferation,
and
differentiation.
study
introduces
novel
surface
modification
designed
engineered
enhance
attachment
cholangiocyte
organoids
facilitate
spreading
cholangiocytes
on
polymer
surface.
Our
findings
revealed
that
recruiting
collagen
layer
polydopamine
(PDA)
nanoparticle
coating
poly(lactide-co-trimethylene
carbonate)
(PLATMC)
plays
key
role
attracting
liver
enhancing
migration.
finding
has
promising
results
generating
two-dimensional
(2D)
monolayer
through
organoid
subsequent
integration.
The
innovative
approach
combines
with
culture
technique,
offering
significant
potential
advancing
bile
duct
regenerative
medicine
developing
more
complex
three-dimensional
(3D)
tissues
vitro.
JACS Au,
Journal Year:
2025,
Volume and Issue:
5(2), P. 550 - 570
Published: Feb. 6, 2025
Cell
surface
engineering
is
a
rapidly
advancing
field,
pivotal
for
understanding
cellular
physiology
and
driving
innovations
in
biomedical
applications.
In
this
regard,
DNA
nanotechnology
offers
unprecedented
potential
precisely
manipulating
functionalizing
cell
surfaces
by
virtue
of
its
inherent
programmability
versatile
functionalities.
Herein,
Perspective
provides
comprehensive
overview
emerging
trends
engineering,
focusing
on
key
nanostructure-based
tools,
their
roles
regulating
physiological
processes,
We
first
discuss
the
strategies
integrating
molecules
onto
surfaces,
including
attachment
oligonucleotides
higher-order
nanostructure.
Second,
we
summarize
impact
DNA-based
various
such
as
membrane
protein
degradation,
signaling
transduction,
intercellular
communication,
construction
artificial
components.
Third,
highlight
applications
DNA-engineered
targeted
therapies
cancer
inflammation,
well
capture/protection
diagnostic
detection.
Finally,
address
challenges
future
directions
nanotechnology-based
engineering.
This
aims
to
provide
valuable
insights
rational
design
contributing
development
precise
personalized
medicine.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 6, 2025
Microtia
is
a
congenital
malformation
characterized
by
underdevelopment
of
the
external
ear.
While
chondrocyte
dysfunction
has
been
implicated
in
microtia,
specific
cellular
abnormalities
remain
poorly
understood.
This
study
aimed
to
investigate
mitochondrial
microtia
chondrocytes
using
single-cell
RNA
sequencing.
Cartilage
samples
were
obtained
from
patients
with
unilateral,
non-syndromic
and
healthy
controls.
Single-cell
sequencing
was
performed
10
×
Genomics
platform.
Bioinformatic
analyses
including
cell
type
identification,
trajectory
analysis,
gene
co-expression
network
analysis
conducted.
Mitochondrial
function
assessed
through
ROS
levels,
membrane
potential,
transmission
electron
microscopy.
Chondrocytes
showed
lower
scores
compared
normal
samples.
Trajectory
revealed
more
disorganized
differentiation
patterns
chondrocytes.
confirmed
increased
production,
decreased
altered
structure.
Gene
identified
hub
genes
associated
function,
SDHA,
SIRT1,
PGC1A,
which
reduced
expression
provides
evidence
identifies
potential
key
involved
this
process.
These
findings
offer
new
insights
into
pathogenesis
may
guide
future
therapeutic
strategies.
Trends in Cell Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
HighlightsA
cell
possesses
competency
if
it
expresses
specific
receptors
and
can
thus
react
to
complementary
signalling
ligands.Signalling
ligands
be
disseminated
via
filopodia
called
cytonemes
exovesicles
such
as
exosomes.Cytonemes
exosomes
also
transport
receiving
cells.Upon
acquiring
transferred
receptors,
cells
the
appropriate
signal
acquire
adoptive
competency.AbstractCellular
communication
through
dissemination
of
molecules
is
vital
for
tissue
organisation
homeostasis.
The
mechanisms
spreading
include
binding-protein-assisted
transport,
long
membrane
protrusions
known
cytonemes,
exovesicles.
Recent
research
indicates
that
not
only
but
facilitate
regulated
distribution
thereby
enabling
transduction
in
lacking
endogenous
receptors.
This
mechanism
allows
non-responsive
temporarily
ability
respond
ligands.
review
explores
our
understanding
ligand
receptor
dispersal,
offering
fresh
insights
into
fundamental
concept
cellular
competence.
Notably,
these
findings
may
have
significant
implications
diseases
their
associated
therapeutic
targets,
highlighting
urgency
importance
this
area.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 29, 2025
Abstract
Detecting
cell-cell
communications
(CCCs)
in
single-cell
transcriptomics
studies
is
fundamental
for
understanding
the
function
of
multicellular
organisms.
Here,
we
introduce
FastCCC,
a
permutation-free
framework
that
enables
scalable,
robust,
and
reference-based
analysis
identifying
critical
CCCs
uncovering
biological
insights.
FastCCC
relies
on
fast
Fourier
transformation-based
convolution
to
compute
p
-values
analytically
without
permutations,
introduces
modular
algebraic
operation
capture
broad
spectrum
CCC
patterns,
can
leverage
atlas-scale
single
cell
references
enhance
user-collected
datasets.
To
support
routine
analysis,
constructed
first
human
reference
panel,
encompassing
19
distinct
tissue
types,
over
450
unique
approximately
16
million
cells.
We
demonstrate
advantages
across
multiple
datasets,
most
which
exceed
analytical
capabilities
existing
methods.
In
real
reliably
captures
biologically
meaningful
CCCs,
even
highly
complex
environments,
including
differential
interactions
between
endothelial
immune
cells
linked
COVID-19
severity,
dynamic
thymic
during
T-cell
development,
as
well
analysis.
npj Precision Oncology,
Journal Year:
2025,
Volume and Issue:
9(1)
Published: Feb. 28, 2025
Abstract
Head
and
neck
squamous
cell
carcinoma
(HNSC)
is
a
prevalent
malignancy,
with
HPV-negative
tumors
exhibiting
aggressive
behavior
poor
prognosis.
Understanding
the
intricate
interactions
within
tumor
microenvironment
(TME)
crucial
for
improving
prognostic
models
identifying
therapeutic
targets.
Using
BulkSignalR,
we
identified
ligand-receptor
in
TCGA-HNSC
cohort
(
n
=
395).
A
model
incorporating
14
pairs
was
developed
using
random
forest
survival
analysis
LASSO-penalized
Cox
regression
based
on
overall
progression-free
interval
of
from
TCGA-HNSC.
Multi-omics
revealed
distinct
molecular
features
between
risk
groups,
including
differences
extracellular
matrix
remodeling,
angiogenesis,
immune
infiltration,
APOBEC
enzyme
activity.
Deep
learning-based
tissue
morphology
HE-stained
whole
slide
images
further
improved
stratification,
region
selection
via
Silicon
enhancing
accuracy.
The
integration
routine
histopathology
deep
learning
multi-omics
data
offers
clinically
accessible
tool
precise
facilitating
personalized
treatment
strategies
HNSC.
