Acidosis regulates immune progression in rheumatoid arthritis by promoting the expression of cytokines and co-stimulatory molecules in synovial fibroblasts DOI Creative Commons

Xuewen Qian,

Zhuoyan Zai,

Yong Tao

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: April 15, 2025

Abstract Background Tissue acidosis is a key characteristic of RA. It remains unclear whether promotes the formation complex adaptive immune landscape mainly characterized by T cell activation in RA influencing synovial fibroblasts. This study aims to investigate influence on microenvironment exploring cytokine secretion and expression co-stimulatory factors Methods The Bulk RNA-seq dataset (GSE89408, Normal = 23, 150) was utilized for screening state assessment based disease stage. employed molecule following 6 h acid stimulation, combined with data evaluate contributions Human arrays were used confirm accumulation supernatants after 12 stimulation. Proteomics applied explore cellular functional states RASFs under stress, joint analysis elucidating transcription factor activation. Validation select high-throughput performed using qRT-PCR immune-based assays. Results identified 56 differentially expressed cytokines at their intersection. Functional enrichment demonstrated that stimulation enhanced chemotaxis fibroblasts (RASFs). Cytokine array revealed exposure increased growth (e.g., FGF, VEGF) over twofold promoted multiple inflammatory chemotactic factors. Immune indicated induced upregulating antigen-presenting molecules. showed stress mitochondrial function triggered metabolic reprogramming RASFs. Integrated transcriptomic proteomic analyses AP1 regulates gene RASFs, its further confirmed Western blotting immunofluorescence.

Language: Английский

Dietary Flavonoid Intake and Anemia Risk in Children and Adolescents: Insights from National Health and Nutrition Examination Survey DOI Creative Commons
Linfeng Li,

Zhongwang Wang,

Zhengyu Yu

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 395 - 395

Published: March 27, 2025

Anemia, a global health concern, significantly impacts children and adolescents, impairing their physical cognitive development. While nutritional deficiencies are primary contributors, oxidative stress has emerged as key factor in anemia pathogenesis. Flavonoids, known for antioxidant properties, may play protective role, but relationship with pediatric populations remains underexplored. Using data from the National Health Nutrition Examination Survey (NHANES) cycles (2007–2008, 2009–2010, 2017–2018), we analyzed 6815 participants aged ≤20 years to investigate association between dietary flavonoid intake risk. Flavonoid was assessed via two 24 h recalls, defined using WHO hemoglobin thresholds. Multivariable logistic regression restricted cubic spline (RCS) models were employed, adjusting sociodemographic, dietary, lifestyle factors. The results showed that lower associated increased High-intake groups of total flavonoids flavan-3-ols linked reduced prevalence compared low-intake groups, odds ratios (ORs) OR = 0.641 (95% CI: 0.439, 0.935) 0.612 0.406, 0.921), respectively. This study highlights potential role flavonoids, particularly flavan-3-ols, reducing risk among underscoring importance flavonoid-rich diets prevention.

Language: Английский

Citations

0
The Citric Acid Cycle: Historical Discovery, Biochemical Mechanisms, and Its Impact on Life Sciences DOI Creative Commons

Lu Lan

Journal of Contemporary Medical Practice, Journal Year: 2025, Volume and Issue: 7(3), P. 187 - 192

Published: March 28, 2025

With the rapid development of metabolomics and systems biology, scientists have made significant strides in exploring regulatory mechanisms citric acid cycle, its associations with various diseases, applications bioengineering. This paper reviews historical discovery analyzes biochemical mechanisms, discusses pivotal role modern life sciences. By examining breakthroughs early metabolic research, particularly advancements glycolysis, metabolism, enzymology, this reveals how cycle was discovered based on foundational work previous studies. It also elaborates scientific breakthrough has propelled theoretical innovations fields metabolism cell biology. Through a comprehensive analysis milestone discovery, aims to highlight fundamental position sciences lasting impact.

Language: Английский

Citations

0
Development and Application of Organic Sonosensitizers in Cancer Therapy DOI Creative Commons
Yuhan Ding,

Yuchen Yang,

Ömer Aras

et al.

Aggregate, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

ABSTRACT Sonodynamic therapy (SDT) is an innovative cancer modality that harnesses the energy of ultrasound to activate sonosensitizers for producing reactive oxygen species (ROS), culminating in eradication tumor cells. Compared with photodynamic therapy, SDT has capacity penetrate deeply into biological tissues, thereby holding significant promise addressing situated or surgically inaccessible tumors. The effectiveness greatly dependent on characteristics sonosensitizers, and unlike inorganic organic offer a more controlled synthesis process have excellent biocompatibility. This review presents meticulous undertaking categorize elucidate their mechanisms action therapeutic effects context SDT. Design strategies are also summarized, we emphasize critical role nanotechnology localization, imaging, multimodal synergistic offering approach achieving precise targeting. In addition, impact delineated when integrated other oncological modalities, such as photothermal enhance efficacy. Finally, discusses challenges future perspectives advancement clinical within realm oncology.

Language: Английский

Citations

0
Sirtuins as modulators of infection outcomes in the battle of host-pathogen dynamics DOI
Dipasree Hajra, Dipshikha Chakravortty

Physics of Life Reviews, Journal Year: 2025, Volume and Issue: 53, P. 225 - 235

Published: March 22, 2025

Language: Английский

Citations

0
Acidosis regulates immune progression in rheumatoid arthritis by promoting the expression of cytokines and co-stimulatory molecules in synovial fibroblasts DOI Creative Commons

Xuewen Qian,

Zhuoyan Zai,

Yong Tao

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Abstract Background: Tissue acidosis is a key characteristic of RA. It remains unclear whether promotes the formation complex adaptive immune landscape mainly characterized by T cell activation in RA influencing synovial fibroblasts. This study aims to investigate influence on microenvironment exploring cytokine secretion and expression co-stimulatory factors Methods: The Bulk RNA-seq dataset (GSE89408, Normal=23, RA=150) was utilized for screening state assessment based disease stage. employed molecule following 6h acid stimulation, combined with data evaluate contributions Human arrays were used confirm accumulation supernatants after 12h stimulation. Proteomics applied explore cellular functional states RASFs under stress, joint analysis elucidating transcription factor activation. Validation select high-throughput performed using qRT-PCR immune-based assays. Results: Bulk identified 56 differentially expressed cytokines at their intersection. Functional enrichment demonstrated that stimulation enhanced chemotaxis RASFs. Cytokine array revealed exposure increased growth (e.g., FGF, VEGF) over twofold promoted multiple inflammatory chemotactic factors. Immune indicated induced upregulating antigen-presenting molecules. showed stress mitochondrial function triggered metabolic reprogramming Integrated transcriptomic proteomic analyses AP1 regulates gene RASFs, its further confirmed Western blotting immunofluorescence.

Language: Английский

Citations

0
Integrative metabolic profiling of hypothalamus and skeletal muscle in a mouse model of cancer cachexia DOI
Jae Yeon Choi, Ye Jin Kim,

Jeong Seob Shin

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 763, P. 151766 - 151766

Published: April 6, 2025

Language: Английский

Citations

0
Acidosis regulates immune progression in rheumatoid arthritis by promoting the expression of cytokines and co-stimulatory molecules in synovial fibroblasts DOI Creative Commons

Xuewen Qian,

Zhuoyan Zai,

Yong Tao

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: April 15, 2025

Abstract Background Tissue acidosis is a key characteristic of RA. It remains unclear whether promotes the formation complex adaptive immune landscape mainly characterized by T cell activation in RA influencing synovial fibroblasts. This study aims to investigate influence on microenvironment exploring cytokine secretion and expression co-stimulatory factors Methods The Bulk RNA-seq dataset (GSE89408, Normal = 23, 150) was utilized for screening state assessment based disease stage. employed molecule following 6 h acid stimulation, combined with data evaluate contributions Human arrays were used confirm accumulation supernatants after 12 stimulation. Proteomics applied explore cellular functional states RASFs under stress, joint analysis elucidating transcription factor activation. Validation select high-throughput performed using qRT-PCR immune-based assays. Results identified 56 differentially expressed cytokines at their intersection. Functional enrichment demonstrated that stimulation enhanced chemotaxis fibroblasts (RASFs). Cytokine array revealed exposure increased growth (e.g., FGF, VEGF) over twofold promoted multiple inflammatory chemotactic factors. Immune indicated induced upregulating antigen-presenting molecules. showed stress mitochondrial function triggered metabolic reprogramming RASFs. Integrated transcriptomic proteomic analyses AP1 regulates gene RASFs, its further confirmed Western blotting immunofluorescence.

Language: Английский

Citations

0