Nitrogen cycling and microbial cooperation in the terrestrial subsurface

The ISME Journal, Journal Year: 2022, Volume and Issue: 16(11), P. 2561 - 2573

Published: Aug. 8, 2022

The nitrogen cycle plays a major role in aquatic transformations, including the terrestrial subsurface. However, variety of transformations remains understudied. To determine how cycling microorganisms respond to different aquifer chemistries, we sampled groundwater with varying nutrient and oxygen contents. Genes transcripts involved nitrogen-cycling pathways were quantified from 55 26 sites, respectively, metagenomes metatranscriptomes analyzed subset oxic dysoxic sites (0.3-1.1 mg/L bulk dissolved oxygen). Nitrogen-cycling mechanisms (e.g. ammonia oxidation, denitrification, dissimilatory nitrate reduction ammonium) prevalent highly redundant, regardless site-specific physicochemistry or availability, present 40% reconstructed genomes, suggesting that is core function communities. Transcriptional activity for nitrification, nitrite-dependent anaerobic methane oxidation (anammox) occurred simultaneously groundwater, indicating availability oxic-anoxic interfaces. Concurrent by these indicates potential synergisms through metabolite exchange across interfaces nitrite Fragmented denitrification pathway encoding transcription was widespread among bacteria, although considerable proportion associated transcriptional driven complete denitrifiers, especially under conditions. Despite large differences transcription, capacity final steps largely invariant conditions, most genes N2O reductases atypical Sec-dependant type, energy-efficiency prioritization. Results provide insights into cooperative relationships communities, richness complexity metabolic leading loss fixed nitrogen.

Language: Английский

---

A critical review on microbial ecology in the novel biological nitrogen removal process: Dynamic balance of complex functional microbes for nitrogen removal

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 857, P. 159462 - 159462

Published: Oct. 17, 2022

Language: Английский

---

Nitrite Oxidation in Wastewater Treatment: Microbial Adaptation and Suppression Challenges

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(34), P. 12557 - 12570

Published: Aug. 17, 2023

Microbial nitrite oxidation is the primary pathway that generates nitrate in wastewater treatment systems and can be performed by a variety of microbes: namely, nitrite-oxidizing bacteria (NOB). Since NOB were first isolated 130 years ago, understanding phylogenetical physiological diversities has been gradually deepened. In recent endeavors advanced biological nitrogen removal, have more considered as troublesome disruptor, strategies on suppression often fail practice after long-term operation due to growth specific are able adapt even harsh conditions. line with review history currently known genera, phylogenetic tree constructed exhibit wide range different phyla. addition, behavior metabolic performance strains summarized. These features various (e.g., high oxygen affinity

Language: Английский

---

A genomic catalogue of soil microbiomes boosts mining of biodiversity and genetic resources

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 11, 2023

Abstract Soil harbors a vast expanse of unidentified microbes, termed as microbial dark matter, presenting an untapped reservoir biodiversity and genetic resources, but has yet to be fully explored. In this study, we conduct large-scale excavation soil matter by reconstructing 40,039 metagenome-assembled genome bins (the SMAG catalogue) from 3304 metagenomes. We identify 16,530 21,077 species-level (SGBs) unknown SGBs (uSGBs), which expand archaeal bacterial diversity across the tree life. also illustrate pivotal role uSGBs in augmenting microbiome’s functional landscape intra-species diversity, providing large proportions 43,169 biosynthetic gene clusters 8545 CRISPR-Cas genes. Additionally, determine that contributed 84.6% previously unexplored viral-host associations catalogue. The catalogue provides useful genomic resource for further studies investigating resources.

Language: Английский

---

Complete ammonia oxidation (comammox) at pH 3-4 supports stable production of ammonium nitrate from urine

Water Research, Journal Year: 2024, Volume and Issue: 257, P. 121686 - 121686

Published: April 28, 2024

This study developed a new process that stably produced ammonium nitrate (NH

Language: Английский

---

Investigating Biofilms: Advanced Methods for Comprehending Microbial Behavior and Antibiotic Resistance

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(4), P. 133 - 133

Published: March 29, 2024

Biofilms, which consist of microorganisms enclosed in an extracellular polymeric material (EPS), hold immense importance the fields environmental research, industry, and medicine. They play a significant role ecosystem dynamics stability, but they also pose issues such as biofouling, corrosion, pollution. Biofilms medical environments are linked to persistent infections elevated healthcare expenses. The EPS matrix plays crucial maintaining structural integrity antibiotic resistance these structures. research primarily investigates facilitating horizontal gene transfer among biofilm communities, with particular emphasis on its impact this process. process is recognized pivotal mechanism emergence resistance, underscoring function biofilms. analysis highlights financial constraints caused by biofilms several industries. Biofilm-associated sector result escalated treatment expenses extended hospitalization periods. In industrial context, have increasing maintenance product contamination, emphasizing need for efficient management solutions. This review presents most recent progress utilization sophisticated imaging tools molecular methodologies. addition conventional techniques, explores tools, DNA RNA sequencing, conjunction proteomics. These approaches essential assessing genetic metabolic mechanisms that regulate development resistance. underscores significance employing interdisciplinary methodology study By incorporating range approaches, analysis, comprehensive understanding may be achieved. approach opens up possibilities developing novel solutions address negative impacts health, environment.

Language: Английский

---

Insights into Nitrous Oxide Mitigation Strategies in Wastewater Treatment and Challenges for Wider Implementation

Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 55(11), P. 7208 - 7224

Published: May 12, 2021

Nitrous oxide (N2O) emissions account for the majority of carbon footprint wastewater treatment plants (WWTPs). Many N2O mitigation strategies have since been developed while a holistic view is still missing. This article reviews state-of-the-art studies in treatment. Through analyzing existing studies, this presents essential knowledge to guide mitigations, and logics behind strategies. In practice, mitigations are mainly carried out by aeration control, feed scheme optimization, process optimization. Despite increasingly more real implementation remains rare, which combined result unclear climate change policies/incentives, as well technical challenges. Five critical challenges, opportunities, were identified. It proposed that (i) quantification methods overall pathway contributions need improvement; (ii) reliable straightforward mathematical model required quantify benefits compare strategies; (iii) tailored risk assessment needs be conducted WWTPs, long-term full-scale trials urgently needed enable robust assessments resulting operational costs impact on nutrient removal performance; (iv) current focus centralized investigations warranted decentralised systems, especially decentralized activated sludge WWTPs; (v) may mitigated adopting novel promoting reduction denitrification or microorganisms emit less N2O. Overall, we conclude research reaching maturity challenges exist wider implementation, relation reliability potential risks performances WWTPs.

Language: Английский

---

Sustained nitrogen loss in a symbiotic association of Comammox Nitrospira and Anammox bacteria

Water Research, Journal Year: 2021, Volume and Issue: 202, P. 117426 - 117426

Published: July 11, 2021

Language: Английский

---

Ammonia-oxidizing archaea and complete ammonia-oxidizing Nitrospira in water treatment systems

Water Research X, Journal Year: 2022, Volume and Issue: 15, P. 100131 - 100131

Published: March 14, 2022

Nitrification, the oxidation of ammonia to nitrate via nitrite, is important for many engineered water treatment systems. The sequential steps this respiratory process are carried out by distinct microbial guilds, including ammonia-oxidizing bacteria (AOB) and archaea (AOA), nitrite-oxidizing (NOB), newly discovered members genus Nitrospira that conduct complete (comammox). Even though all these nitrifiers have been identified within systems, their relative contributions nitrogen cycling poorly understood. Although AOA contribute nitrification in wastewater plants, they generally outnumbered AOB. In contrast, comammox typically dominate relatively low environments such as drinking treatment, tertiary aquaculture/aquarium filtration. Studies focus on abundance oxidizers may misconstrue actual role nitrifying guilds play a system. Understanding which active useful further optimization systems rely removal. This review highlights known distributions suggests future research directions will help assess identify factors influence activity.

Language: Английский

---

Revealing taxon-specific heavy metal-resistance mechanisms in denitrifying phosphorus removal sludge using genome-centric metaproteomics

Microbiome, Journal Year: 2021, Volume and Issue: 9(1)

Published: March 22, 2021

Abstract Background Denitrifying phosphorus removal sludge (DPRS) is widely adopted for nitrogen and in wastewater treatment but faces threats from heavy metals. However, a lack of understanding the taxon-specific metal-resistance mechanisms hinders targeted optimization DPRS’s robustness nutrient removal. Results We obtained 403 high- or medium-quality metagenome-assembled genomes DPRS treated by elevating cadmium, nickel, chromium pressure. Then, proteomic responses individual taxa under metal pressures were characterized, with an emphasis on functions involving resistance maintenance metabolism. When oxygen availability was constrained high-concentration metals, comammox Nitrospira overproduced highly oxygen-affinitive hemoglobin electron-transporting cytochrome c-like proteins, underpinning its ability to enhance acquisition utilization. In contrast, Nitrosomonas overexpressed ammonia monooxygenase nitrite reductase facilitate partial nitrification denitrification process maintaining Comparisons between phosphorus-accumulating organisms (PAOs) demonstrated different Dechloromonas Candidatus Accumulibacter, despite their high genomic similarities. particular, outcompeted canonical PAO Accumulibacter synthesizing polyphosphate, potential public good detoxification. The superiority energy utilization, radical elimination, damaged cell component repair also contributed dominance pressures. Moreover, enrichment analysis revealed that involved extracellular polymeric substance formation, siderophore activity, efflux significantly due related activities specific taxa. Conclusions Our study demonstrates within multipartite community are heterogeneous These findings provide fundamental how heterogeneity microorganisms contributes metabolic versatility microbiomes inhabiting dynamic environments, which vital manipulating adaptation microbial assemblages adverse environmental stimuli.

Language: Английский

---