Enhanced surface colonisation and competition during bacterial adaptation to a fungus

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 27, 2024

Abstract Bacterial-fungal interactions influence microbial community performance of most ecosystems and elicit specific behaviours, including stimulating specialised metabolite production. Here, we use a co-culture experimental evolution approach to investigate bacterial adaptation the presence fungus, using simple model bacterial-fungal encompassing bacterium Bacillus subtilis fungus Aspergillus niger . We find in one evolving population that B. was selected for enhanced production lipopeptide surfactin accelerated surface spreading ability, leading inhibition fungal expansion acidification environment. These phenotypes were explained by mutations DegS-DegU two-component system. In surfactin, hyphae exhibited bulging cells with delocalised secretory vesicles possibly provoking an RlmA-dependent cell wall stress. Thus, our results indicate selects increased production, which inhibits growth facilitates competitive success bacterium.

Language: Английский

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Frenemies of the soil: Bacillus and Pseudomonas interspecies interactions

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 31(8), P. 845 - 857

Published: March 4, 2023

Bacillus and Pseudomonas ubiquitously occur in natural environments are two of the most intensively studied bacterial genera soil. They often coisolated from environmental samples, as a result, several studies have experimentally cocultured bacilli pseudomonads to obtain emergent properties. Even so, general interaction between members these is virtually unknown. In past decade, data on interspecies interactions isolates has become more detailed, now, molecular permit mapping mechanisms behind their pairwise ecology. This review addresses current knowledge about microbe–microbe strains discusses how we can attempt generalize taxonomic level.

Language: Английский

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Breakdown of clonal cooperative architecture in multispecies biofilms and the spatial ecology of predation

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(6)

Published: Feb. 2, 2023

Biofilm formation, including adherence to surfaces and secretion of extracellular matrix, is common in the microbial world, but we often do not know how interaction at cellular spatial scale translates higher-order biofilm community ecology. Here explore an especially understudied element ecology, namely predation by bacterium Bdellovibrio bacteriovorus . This predator can kill consume many different Gram-negative bacteria, Vibrio cholerae Escherichia coli V. protect itself from within densely packed structures that it creates, whereas E. biofilms are highly susceptible B. We predator–prey dynamics change when growing together. find dual-species prey biofilms, survival under increases, decreases. benefits protection becomes embedded expanding groups But also ordered, packed, clonal structure be disrupted if cells directly adjacent start growth. When this occurs, two species become intermixed, resulting disordered cell block entry. Because group depends on initial distributions growth, surface colonization have a dramatic impact eventual multispecies architecture, which turn determines what extent both survive exposure bacteriovorus.

Language: Английский

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Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection

Cell Host & Microbe, Journal Year: 2023, Volume and Issue: 31(10), P. 1639 - 1654.e10

Published: Sept. 29, 2023

Language: Английский

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Competition for iron shapes metabolic antagonism betweenBacillus subtilisandPseudomonas marginalis

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract Siderophores have long been implicated in sociomicrobiology as determinants of bacterial interrelations. For plant-associated genera, like Bacillus and Pseudomonas, siderophores are well known for their biocontrol functions. Here, we explored the functional role subtilis siderophore bacillibactin (BB) an antagonistic interaction with Pseudomonas marginalis. The presence BB strongly influenced outcome iron-dependent manner. producer B. restricts colony spreading P. marginalis by repressing transcription histidine kinase-encoding gene gacS, thereby abolishing production secondary metabolites such pyoverdine viscosin. By contrast, lack restricted growth. To explore specificity antagonism, cocultured a collection fluorescent spp. found that Bacillus–Pseudomonas is conserved, expanding our understanding interplay between two most well-studied genera soil bacteria.

Language: Английский

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Bacillus lipopeptides as key players in rhizosphere chemical ecology

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 33(1), P. 80 - 95

Published: Aug. 29, 2024

HighlightsCyclic lipopeptides (CLPs) represent unique bacterial natural products with an amazing chemical diversity driving multiple potent bioactivities.More than just lubricants, solubilizers or killing agents, CLPs retain unsuspected array of functions and feature as private tools shared goods produced on demand.Resolving the molecular rules CLP selectivity for a given function requires combined expertise in biology, structural chemistry, biophysics to comprehensively understand their intricate interactions biological membranes.Discovering new should exploit multilevel microbial involve recent advances metagenomics, genome mining, community metabolomicsDeciphering ultimate proximate aspects lipopeptide science highlights relevance ecology.AbstractMicrobial are widely explored therapeutic potential. Understanding underlying evolutionary adaptive forces production remains fundamental question biology. Amphiphilic cyclic (CLPs), prominent category specialized metabolites, show strong antimicrobial activity, particularly against phytopathogens. It is thus assumed that these compounds deployed by soil- rhizosphere-dwelling bacteria weapons competitive environments. Here, we challenge this reductionist perspective present evidence Bacillus mediators ecological interactions. They help communicate, compete, defend predators, cooperate establish mutualistic relationships other (micro)organisms. Additional parallel examples highlighted genera, such Pseudomonas. This broader underscores need further investigation into role shaping strategies key rhizobacterial species.

Language: Английский

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Effects of Bacillus subtilis and Pseudomonas fluorescens as the soil amendment

Heliyon, Journal Year: 2022, Volume and Issue: 8(11), P. e11674 - e11674

Published: Nov. 1, 2022

The application of soil beneficial bacteria (SBB) in agriculture is steadily increasing as it provides a promising way to replace chemical fertilisers and other supplements. Although the role SBB biofertiliser well understood, little known about response physiochemical properties via change enzymatic activities with growth. In this study, sterilised bulk was inoculated Bacillus subtilis (BS) Pseudomonas fluorescens (PF), which exhibit excellent characteristics vitro for potentially improving quality. It found that contents bioavailable nitrogen ammonium increased significantly, up 34% 57% relative control. This resulted from enhancement urease activity BS PF treatments by approximately 90% 70%, respectively. can be explained microorganism evident larger population size (0.78–0.97 CFU mL−1/CFU mL−1) than (0.55–0.79 (p < 0.05). Results principal component analysis also reinforce interaction apparent significant relationship between microbial biomass carbon Therefore, concluded induced fertility upregulation because bacterial These results demonstrate strategy amendment, particularly nutrient restoration.

Language: Английский

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Eco-evolutionary modelling of microbial syntrophy indicates the robustness of cross-feeding over cross-facilitation

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Jan. 17, 2023

Syntrophic cooperation among prokaryotes is ubiquitous and diverse. It relies on unilateral or mutual aid that may be both catalytic metabolic in nature. Hypotheses of eukaryotic origins claim mitochondrial endosymbiosis emerged from mutually beneficial syntrophy archaeal bacterial partners. However, there are no other examples prokaryotic leading to endosymbiosis. One potential reason when externalized products become public goods, they incite social conflict due selfish mutants undermine any mutualistic interactions. To rigorously evaluate these arguments, here we construct a general mathematical framework the ecology evolution different types syntrophic partnerships. We do so microbial eukaryogenetic context. Studying case where partners cross-feed each other's self-inhibiting waste, show cooperative partnerships will eventually dominate over mutants. By contrast, systems producers actively secrete enzymes cross-facilitate their partners' resource consumption not robust against cheaters evolutionary time. conclude cross-facilitation unlikely provide an adequate origin for endosymbiosis, but cross-feeding mutualisms indeed have played role.

Language: Английский

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Antibiofilm Activity of the Marine Probiotic Bacillus subtilis C3 Against the Aquaculture-Relevant Pathogen Vibrio harveyi

Probiotics and Antimicrobial Proteins, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Language: Английский

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Interfacial Morphodynamics of Proliferating Microbial Communities

Physical Review X, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 29, 2025

In microbial communities, various cell types often coexist by occupying distinct spatial domains. What determines the shape of interface between such domains—which, in turn, influences interactions cells and overall community function? Here, we address this question developing a continuum model 2D spatially structured with two types. We find that, depending on balance different proliferation rates substrate friction coefficients, domains is either stable smooth or unstable develops fingerlike protrusions. establish quantitative principles describing when these interfacial behaviors arise good agreement both results previous experimental reports as well new experiments performed here. Our work, thus, helps to provide biophysical basis for understanding morphodynamics proliferating communities broader range active systems. Published American Physical Society 2025

Language: Английский

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