Mechanistic understanding of acclimation and energy metabolism of acetoclastic methanogens under different substrate to microorganism ratios

Environmental Research, Journal Year: 2024, Volume and Issue: 252, P. 118911 - 118911

Published: April 10, 2024

Mechanistic understanding of acetoclastic methanogenesis is pivotal for optimizing anaerobic digestion efficient methane production. In this study, two different operational modes, continuous flow reactor (CFR) and sequencing batch (SBR), accompanied with solids retention times (SRT) 10 days (SBR10d CFR10d) 25 (SBR25d CFR25d) were implemented to elucidate their impacts on microbial communities energy metabolism methanogens in acetate-fed systems. Microbial community analysis revealed that the relative abundance Methanosarcina (16.0% ∼ 46.0%) surpassed Methanothrix (3.7% 22.9%) each reactor. SBRs had potential enrich both Methanosarcina. Compared SBRs, CFRs lower total methanogens. exhibited a superior enrichment reactors 10-day SRT, while preferred be acclimated 25-day SRT. The mode SRT also observed affect distribution acetate-utilizing bacteria, including Pseudomonas, Desulfocurvus, Mesotoga, Thauera. Regarding enzymes involved metabolism, Ech Vho/Vht demonstrated higher abundances at compared whereas Fpo MtrA-H showed than those CFRs. genes encoding ATPase harbored by was Additionally, V/A-type (typically methanogens) CFRs, F-type bacteria) SBRs.

Language: Английский

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Harnessing microbial division of labor for biomanufacturing: a review of laboratory and formal modeling approaches

Critical Reviews in Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 19

Published: Feb. 19, 2025

Bioprocess industries aim to meet the increasing demand for product complexity by designing enhanced cellular and metabolic capabilities host. Monocultures, standard biomanufacturing workhorses, are often restricted in their capability these demands, solution involves genetic modification of Synthetic microbial communities a promising alternative monocultures because they exhibit division labor, enabling efficient resource utilization pathway modularity. This specialization minimizes burden enhances robustness perturbations, providing competitive advantage. Despite this potential, biotechnological or bioprocessing applications remains limited. The recent emergence new innovative community design tools strategies, particularly those harnessing holds promise change outlook. Understanding interactions governing natural can be used identify complementary partners, informing synthetic design. Therefore, we consider engineering labor as viable accelerate progress field. review presents current understanding how enable information perform tasks otherwise unfeasible individual organisms. We then evaluate laboratory formal modeling approaches specifically developed to: elucidate physiology, guide experimental design, improve our complex assisting By synthesizing insights, present comprehensive framework that advances use applications.

Language: Английский

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Simultaneous biogas upgrading and single cell protein production using hydrogen oxidizing bacteria

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 490, P. 151576 - 151576

Published: April 23, 2024

Language: Английский

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Viral Auxiliary Roles in Hydrolytic and Biosynthetic Metabolism Regulate Prokaryotic Microbial Interactions in Anaerobic Digestion

Water Research, Journal Year: 2025, Volume and Issue: 274, P. 123140 - 123140

Published: Jan. 13, 2025

Language: Английский

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A co-feeding strategy of formate and H2 for methanogens – Enhancing growth parameters and methane production

Journal of CO2 Utilization, Journal Year: 2025, Volume and Issue: 93, P. 103049 - 103049

Published: March 1, 2025

Language: Английский

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Metagenomic Exploration Uncovers Several Novel ‘Candidatus’ Species Involved in Acetate Metabolism in High‐Ammonia Thermophilic Biogas Processes

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(3)

Published: March 1, 2025

ABSTRACT Biogas reactors operating at elevated ammonia levels are commonly susceptible to process disturbances, further augmented thermophilic temperatures. The major cause is assumed be linked inhibition followed by an imbalance between different functional microbial groups, centred around the last two steps of anaerobic digestion, involving acetogens, syntrophic acetate oxidisers (SAOB) and methanogens. Acetogens key contributors reactor efficiency, acting as crucial link hydrolysis fermentation final methanogenesis step. Their product acetate, high converted SAOB hydrogenotrophic methanogens biogas. Even though these functionally processes well recognised, less known about responsible organism temperature conditions. main aim this study was garner insights into penultimate stages in three (52°C) operated under (FAN 0.7–1.0 g/L; TAN 3.6–4.4 g/L). primary objective identify potential acetogens SAOBs. Metagenomic data from were analysed for reductive acetyl‐CoA pathway (Wood–Ljungdahl Pathway) glycine synthase reductase pathway. results revealed a lack true but uncovered candidates that harbour WLP, ‘ Candidatus Thermodarwinisyntropha acetovorans’, Thermosyntrophaceticus schinkii’, Thermotepidanaerobacter aceticum’, lipid‐degrader Thermosyntrophomonas ammoiaca’.

Language: Английский

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Nine (not so simple) steps: a practical guide to using machine learning in microbial ecology

mBio, Journal Year: 2023, Volume and Issue: 15(2)

Published: Dec. 21, 2023

Due to the complex nature of microbiome data, field microbial ecology has many current and potential uses for machine learning (ML) modeling. With increased use predictive ML models across disciplines, including ecology, there is extensive published information on specific algorithms available how those have been applied. Thus, our goal not summarize breadth or compare their performances. Rather, provide more concrete actionable guide ecologists in select, run, interpret predict taxa genes associated with particular sample categories environmental gradients interest. Such data often unique characteristics that require careful consideration apply results. This review intended practicing who may be unfamiliar some intricacies models. We examples discuss common opportunities pitfalls applying types sets most frequently collected by ecologists.

Language: Английский

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Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics

Microorganisms, Journal Year: 2023, Volume and Issue: 11(10), P. 2412 - 2412

Published: Sept. 27, 2023

The current focus on renewable energy in global policy highlights the importance of methane production from biomass through anaerobic digestion (AD). To improve while ensuring overall process stability, microbiome-based management strategies become more important. In this study, metagenomes and metaproteomes were used for metagenomically assembled genome (MAG)-centric analyses to investigate a full-scale biogas plant consisting three differentially operated digesters. Microbial communities analyzed regarding their taxonomic composition, functional potential, as well functions expressed proteome level. Different abundances genes enzymes related could be mostly attributed different parameters. Individual MAGs exhibiting digesters studied detail, roles hydrolysis, acidogenesis acetogenesis steps assigned.

Language: Английский

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Carbon/nitrogen flows and associated microbial communities in full-scale foodwaste treatment plants

Bioresource Technology, Journal Year: 2023, Volume and Issue: 388, P. 129775 - 129775

Published: Sept. 16, 2023

Language: Английский

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Feedstock-dependent antibiotic resistance gene patterns and expression profiles in industrial scale biogas plants revealed by meta-omics technology

Water Research, Journal Year: 2024, Volume and Issue: 268, P. 122650 - 122650

Published: Oct. 18, 2024

Language: Английский

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