Enhancing pancreatic cancer treatment: the role of H101 oncolytic virus in irreversible electroporation

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 18, 2025

Irreversible Electroporation (IRE) offers a promising treatment for pancreatic cancer by using high-voltage pulses to kill tumor cells. But variations in size and shape can lead uneven electric fields, causing some cells undergo only reversible electroporation (RE) survive. However, RE temporarily increase the permeability of cell membrane, allowing small molecules enter. H101 virus is an oncolytic adenovirus with deleted E1B-55kD E3 regions that selectively targets kills This study aimed investigate whether serve as supplementary therapeutic approach tumors combined RE. We first explored how virus, both individually together, affected proliferation migration cellular experiments. Subsequent vitro studies further assessed effects different treatments on growth. To understand mechanisms pathway changes from groups, we analyzed samples each group bulk RNA sequencing (bulk RNA-seq) single-cell (scRNA-seq). Additional biochemical techniques were used validate key molecular changes. The combination effectively inhibited migration. Experiments mouse subcutaneous models confirmed therapy significantly reduced Further analysis RNA-seq scRNA-seq revealed this activates JNK-MAPK pathway, inducing apoptosis enhancing effects. boosts effectiveness activating promoting apoptosis. These findings suggest could valuable adjunct improve efficacy IRE treatment.

Language: Английский

Recent advances in oncolytic virus combined immunotherapy in tumor treatment

Genes & Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 101599 - 101599

Published: March 1, 2025

Language: Английский