Journal of Inflammation Research,
Journal Year:
2024,
Volume and Issue:
Volume 17, P. 8965 - 8984
Published: Nov. 1, 2024
Ferroptosis
is
an
underlying
mechanism
for
various
degenerative
diseases,
but
its
role
in
intervertebral
disc
degeneration
remains
elusive.
This
study
aims
to
explore
the
key
ferroptosis-related
genes
and
nucleus
pulposus
(NP)
annulus
fibrosus
(AF)
degeneration.
We
analyzed
gene
expression
profiles
of
NP
AF
from
Gene
Expression
Omnibus
database.
The
differentially
expressed
(FRDEGs)
degenerated
were
filtered,
followed
by
GO
KEGG
analysis.
Feature
FRDEGs
identified
LASSO
SVM-RFE
algorithms,
then
Set
Enrichment
Analysis
(GSEA)
Variation
(GSVA)
conducted.
Immune
infiltration
analysis
was
conducted
CIBERSORT
algorithm.
established
drug
networks
via
Drug-Gene
Interaction
Database
competitive
endogenous
RNA
(ceRNA)
miRanda,
miRDB,
TargetScan
levels
feature
assessed
validation
sets,
single-cell
RNA-seq,
experimental
verification.
A
total
15
18
obtained
AF,
respectively.
revealed
their
implication
oxidative
stress.
Four
(AKR1C1,
AKR1C3,
MUC1,
ENPP2)
five
(SCP2,
ABCC1,
KLF2,
IDO1,
CREB3)
GSEA
GSVA
showed
that
these
enriched
lots
biological
functions,
including
immune
response.
CREB3
negatively
correlated
with
Eosinophils
drugs
ceRNAs
targeting
MUC1
identified.
Experimental
verification
RNA-seq
downregulated
considered
novel
biomarkers
ferroptosis,
Drug
ceRNA
constructed
future
development
investigation
new
mechanisms
ferroptosis.
Phytotherapy Research,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 8, 2024
Degenerative
bone
and
joint
diseases
(DBJDs),
characterized
by
osteoporosis,
osteoarthritis,
chronic
inflammation
of
surrounding
soft
tissues,
are
systemic
conditions
primarily
affecting
the
skeletal
system.
Ferroptosis,
a
programmed
cell
death
pathway
distinct
from
apoptosis,
autophagy,
necroptosis.
Accumulating
evidence
suggests
that
ferroptosis
is
intricately
linked
to
pathogenesis
DBJDs,
targeting
its
regulation
could
be
beneficial
in
managing
these
conditions.
Natural
products,
known
for
their
anti-inflammatory
antioxidant
properties,
have
shown
unique
advantages
preventing
potentially
through
modulating
ferroptosis.
This
article
provides
an
overview
latest
research
on
ferroptosis,
with
focus
role
DBJDs
therapeutic
potential
natural
products
this
pathway,
offering
novel
insights
prevention
treatment
DBJDs.
Journal of Inflammation Research,
Journal Year:
2024,
Volume and Issue:
Volume 17, P. 8965 - 8984
Published: Nov. 1, 2024
Ferroptosis
is
an
underlying
mechanism
for
various
degenerative
diseases,
but
its
role
in
intervertebral
disc
degeneration
remains
elusive.
This
study
aims
to
explore
the
key
ferroptosis-related
genes
and
nucleus
pulposus
(NP)
annulus
fibrosus
(AF)
degeneration.
We
analyzed
gene
expression
profiles
of
NP
AF
from
Gene
Expression
Omnibus
database.
The
differentially
expressed
(FRDEGs)
degenerated
were
filtered,
followed
by
GO
KEGG
analysis.
Feature
FRDEGs
identified
LASSO
SVM-RFE
algorithms,
then
Set
Enrichment
Analysis
(GSEA)
Variation
(GSVA)
conducted.
Immune
infiltration
analysis
was
conducted
CIBERSORT
algorithm.
established
drug
networks
via
Drug-Gene
Interaction
Database
competitive
endogenous
RNA
(ceRNA)
miRanda,
miRDB,
TargetScan
levels
feature
assessed
validation
sets,
single-cell
RNA-seq,
experimental
verification.
A
total
15
18
obtained
AF,
respectively.
revealed
their
implication
oxidative
stress.
Four
(AKR1C1,
AKR1C3,
MUC1,
ENPP2)
five
(SCP2,
ABCC1,
KLF2,
IDO1,
CREB3)
GSEA
GSVA
showed
that
these
enriched
lots
biological
functions,
including
immune
response.
CREB3
negatively
correlated
with
Eosinophils
drugs
ceRNAs
targeting
MUC1
identified.
Experimental
verification
RNA-seq
downregulated
considered
novel
biomarkers
ferroptosis,
Drug
ceRNA
constructed
future
development
investigation
new
mechanisms
ferroptosis.
