Challenges and future perspectives in using mesenchymal stem cells for efficient bone fracture healing DOI Creative Commons

Dong Han,

Weiwei Liu,

Jinpeng Gong

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: May 30, 2025

Mesenchymal stem cells (MSCs) demonstrate considerable potential for enhancing bone fracture healing due to their multipotency and immunomodulatory properties. This review investigates the relationship between MSCs, immune system, skeletal microenvironment, focusing on roles of cytokines signaling pathways in osteogenesis. The process fractures is complex involves a coordinated response from various cell types, including which secrete bioactive molecules that promote tissue regeneration modulate inflammation. Despite promise, challenges such as variability MSC sources, ethical considerations, regulatory restrictions, obstacles achieving effective delivery retention at sites restrict clinical application. Recent advancements MSC-based therapies, innovative biomaterials, three-dimensional bioprinting, gene editing technologies, aim improve therapeutic efficacy MSCs. In addition, strategies rejuvenate aged MSCs enhance regenerative capabilities are critical addressing age-related fractures, functionality declines with age. Understanding mechanisms underlying action, paracrine interaction essential optimizing use. Addressing existing limitations research application provides comprehensive perspective future therapies repair. discusses transformative medicine orthopedics, highlighting need further unlock full outcomes patients injuries.

Language: Английский

Biomineral/VEGF-functionalized fiber - enhanced 3D printed GelMA hydrogel to facilitate bone regeneration through osteogenesis and angiogenesis modulation DOI
Xiaokang Liu, Baoxiu Wang, Jinghong Ma

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143991 - 143991

Published: May 1, 2025

Language: Английский

Citations

0
Transcriptomic Profiling of Hypoxia-Adaptive Responses in Tibetan Goat Fibroblasts DOI Creative Commons

Lin Tang,

Li Zhu,

Basang Zhuzha

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(10), P. 1407 - 1407

Published: May 13, 2025

The Tibetan goat (Capra hircus) exhibits remarkable adaptations to high-altitude hypoxia, yet the molecular mechanisms remain unclear. This study integrates RNA-seq, WGCNA, and machine learning explore gene-environment interactions (G × E) in hypoxia adaptation. Fibroblasts from Yunling were cultured under hypoxic (1% O2) normoxic (21% conditions, respectively. identified 68 breed-specific (G), 100 oxygen-responsive (E), 620 interaction-driven (I) Differentially Expressed Genes (DEGs). notably higher number of DEGs compared other effects highlights transcriptional plasticity. We defined two gene sets: Environmental Stress (n = 632, E ∪ I) Genetic Adaptation 659, G I). former significantly enriched pathways related oxidative stress defense metabolic adaptation, while latter showed prominent enrichment associated with vascular remodeling regulation. CTNNB1 emerged as a key regulatory factor both sets, interacting CASP3 MMP2 form core protein-protein interaction (PPI) network. Machine MAP3K5, TGFBR2, RSPO1 ITGB5 critical genes. WGCNA modules where FOXO3, HEXIM1, PPARD promote stabilization HIF-1α adaptation through HIF-1 signaling pathway glycolysis. These findings underscore pivotal role offering novel perspectives for livestock breeding programs biomedical research initiatives.

Language: Английский

Citations

0
Challenges and future perspectives in using mesenchymal stem cells for efficient bone fracture healing DOI Creative Commons

Dong Han,

Weiwei Liu,

Jinpeng Gong

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: May 30, 2025

Mesenchymal stem cells (MSCs) demonstrate considerable potential for enhancing bone fracture healing due to their multipotency and immunomodulatory properties. This review investigates the relationship between MSCs, immune system, skeletal microenvironment, focusing on roles of cytokines signaling pathways in osteogenesis. The process fractures is complex involves a coordinated response from various cell types, including which secrete bioactive molecules that promote tissue regeneration modulate inflammation. Despite promise, challenges such as variability MSC sources, ethical considerations, regulatory restrictions, obstacles achieving effective delivery retention at sites restrict clinical application. Recent advancements MSC-based therapies, innovative biomaterials, three-dimensional bioprinting, gene editing technologies, aim improve therapeutic efficacy MSCs. In addition, strategies rejuvenate aged MSCs enhance regenerative capabilities are critical addressing age-related fractures, functionality declines with age. Understanding mechanisms underlying action, paracrine interaction essential optimizing use. Addressing existing limitations research application provides comprehensive perspective future therapies repair. discusses transformative medicine orthopedics, highlighting need further unlock full outcomes patients injuries.

Language: Английский

Citations

0