LncRNA NCK1-AS1 Aggravates Hepatocellular Carcinoma by the miR-22-3p/YARS Axis to Activate PI3K/AKT Signaling DOI Open Access

Weixin Zhou,

Jie Wang, Jie Zhang

et al.

Journal of Gastrointestinal and Liver Diseases, Journal Year: 2022, Volume and Issue: 31(1), P. 48 - 59

Published: March 19, 2022

Background: Hepatocellular carcinoma (HCC) is frequently diagnosed at late stages when curative treatments are no more appliable. Many studies have proved the active role of long non-coding RNAs (lncRNAs) in cancers’ biology; here, functional lncRNA NCK1-AS1 HCC was identified.Methods: Gene expression tumor tissues evaluated by examining online databases and 88 collected samples from our hospital. The interactions miR-22-3p with tyrosyl-tRNA synthetase (YARS) were tested conducting bioinformatics analysis, luciferase report, RNA pulldown experiments. CCK-8, colony formation, flow cytometry, wound healing, transwell experiments used to dissect NCK1-AS1/miR-22-3p/YARS axis HCC.Results: overexpressed cells tissues. Functional assays depicted that depletion hampered malignant character cells. controlled availability miR-22-3p, resulting YARS upregulation. found a clinical value for diagnosis. Moreover, rescue revealed inhibition or overexpression partially blocked function deficiency As downstream signaling pathway, we discovered activated PI3K/AKT miR-22-3p/YARS axis.Conclusion: present study verified could promote progression via activate signaling.

Language: Английский

CELF Family Proteins in Cancer: Highlights on the RNA-Binding Protein/Noncoding RNA Regulatory Axis DOI Open Access
Maryam Nasiri Aghdam, Texali-Candelaria Garcia-Garduño, Luis Felipe Jave‐Suárez

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(20), P. 11056 - 11056

Published: Oct. 14, 2021

Post-transcriptional modifications to coding and non-coding RNAs are unquestionably a pivotal way in which human mRNA protein diversity can influence the different phases of transcript's life cycle. CELF (CUGBP Elav-like family) proteins RBPs (RNA-binding proteins) with pleiotropic capabilities RNA processing. Their responsibilities extend from alternative splicing transcript editing nucleus stability, translation into cytoplasm. In this way, family members have been connected global alterations cancer proliferation invasion, leading their identification as potential tumor suppressors or even oncogenes. Notably, genetic variants, splicing, phosphorylation, acetylation, subcellular distribution, competition other RBPs, ultimately lncRNAs, miRNAs, circRNAs all impact regulation. Discoveries emerged about control functions, particularly via noncoding RNAs, identified competing, antagonizing, regulating agents biogenesis. On hand, CELFs an intriguing example through broaden our understanding RBP/noncoding regulatory axis. Balancing these complex pathways is undeniably deserves further research. This review outlines some mechanisms regulation functional consequences physiology.

Language: Английский

Citations

31
Serum exosomal long noncoding RNA CRNDE as a prognostic biomarker for hepatocellular carcinoma DOI Creative Commons
Ting Wang,

Hengkai Zhu,

Min Xiao

et al.

Journal of Clinical Laboratory Analysis, Journal Year: 2021, Volume and Issue: 35(11)

Published: Oct. 6, 2021

Accumulating evidence has shown that long noncoding RNA (lncRNA) CRNDE functions as an oncogene in many cancer types. However, its clinical value not yet been explored hepatocellular carcinoma (HCC).A total of 166 patients with HCC and 100 healthy volunteers were enrolled this study. The expression levels serum exosomal lncRNA detected controls by quantitative real-time PCR (qRT-PCR).The significantly increased compared normal controls. High was associated tumor size, differentiation, TNM stage. Receiver operating characteristic (ROC) analysis revealed area under the ROC curve (AUC) 0.839, a sensitivity specificity 69.3% 85.0%. In addition, overall survival (OS) disease-free (DFS) longer lower to those higher expression. Moreover, cirrhosis had worse OS DFS than without cirrhosis. Univariate multivariate analyses indicated high independent indicator poor prognosis.Taken together, might serve potential biomarker for diagnosis prognosis.

Language: Английский

Citations

30
CRNDE: A valuable long noncoding RNA for diagnosis and therapy of solid and hematological malignancies DOI Creative Commons
Xuefei Ma, Wen Jin, Chaoxian Zhao

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2022, Volume and Issue: 28, P. 190 - 201

Published: March 9, 2022

Colorectal neoplasia differentially expressed (CRNDE) is an oncogenic long noncoding RNA (lncRNA). Increased CRNDE expression was initially discovered in colorectal cancer and then a variety of solid tumors hematological malignancies. participates multiple biological processes, such as cell proliferation, differentiation, migration, apoptosis. has been shown to modulate target gene through mechanisms, including transcriptional regulation, post-transcriptional competition for microRNA (miRNA) binding. In this review, we summarize the evidence that supports diagnosis prognosis predicting cancers. The functional roles molecular mechanisms are further described major types therapeutic potential research development also discussed.

Language: Английский

Citations

21
LINC01468 drives NAFLD-HCC progression through CUL4A-linked degradation of SHIP2 DOI Creative Commons
Hongquan Wang, Yan Wang, Shihui Lai

et al.

Cell Death Discovery, Journal Year: 2022, Volume and Issue: 8(1)

Published: Nov. 7, 2022

Accumulating evidence suggests that long noncoding RNAs (lncRNAs) are deregulated in hepatocellular carcinoma (HCC) and play a role the pathogenesis of non-alcoholic fatty liver disease (NAFLD). However, current understanding lncRNAs NAFLD-associated HCC is limited. In this study, transcriptomic profiling analysis three paired human samples from patients with NAFLD-driven adjacent showed LINC01468 expression was significantly upregulated. vitro vivo gain- loss-of-function experiments promotes proliferation cells through lipogenesis. Mechanistically, binds SHIP2 cullin 4 A (CUL4A)-linked ubiquitin degradation, thereby activating PI3K/AKT/mTOR signaling pathway, resulting promotion de novo lipid biosynthesis progression. Importantly, inhibitor reversed sorafenib resistance induced by overexpression. Moreover, ALKBH5-mediated N6-methyladenosine (m6A) modification led to stabilization upregulation RNA. Taken together, findings indicated novel mechanism which LINC01468-mediated lipogenesis progression CUL4A-linked degradation SHIP2. acts as driver NAFLD, highlights potential LINC01468-SHIP2 axis therapeutic target for HCC.

Language: Английский

Citations

21
APOB is a potential prognostic biomarker in hepatocellular carcinoma DOI Creative Commons
Zhifeng Lin, Xiaohui Ji,

Nana Tian

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 3, 2024

Abstract Hepatocellular carcinoma (HCC) is significantly associated with adverse prognostic outcomes. The development and progression of different types human tumors are influenced by APOB. Nevertheless, the significance pathomechanisms APOB in HCC have not been conclusively determined. We assessed expression levels using three publicly available databases TIMER2.0, UALCAN Human Protein Atlas. To identify biological function APOB, we conducted enrichment analysis via LinkedOmics. Moreover, was employed to assess relationship between clinicopathological features among patients. Additionally, Kaplan–Meier plotter utilized investigate relevance HCC. explore potential regulatory ncRNAs that could bind StarBase GEPIA. Furthermore, correlation immune cell infiltration, as well checkpoint genes, investigated Spearman's TISIDB, GEPIA, TIMER2.0. findings our investigation showed a notable decrease individuals diagnosed noteworthy observed alongside occurrence infiltration. tissues also correlations various features. According Cox regression analysis, decreased emerged autonomous predictor for OS, RFS, DSS, PFS identified six pathways non-coding RNA (ncRNA) most promising pathway Our results illuminate possible involvement offer understanding into its governing mechanisms medical importance.

Language: Английский

Citations

4
N6-methyladenosine-mediated CELF2 regulates CD44 alternative splicing affecting tumorigenesis via ERAD pathway in pancreatic cancer DOI Creative Commons
Shihui Lai, Yan Wang, Ting Li

et al.

Cell & Bioscience, Journal Year: 2022, Volume and Issue: 12(1)

Published: Aug. 8, 2022

Alternative splicing (AS) of genes has been found to affect gene stability, and its abnormal regulation can lead tumorigenesis. CELF2 is a vital factor participate in mRNA alternative splicing. Its downregulation confirmed promote the occurrence development pancreatic cancer (PC). However, regulatory role mechanisms PC not elucidated. was downregulated tissues, which affected tumor TNM stage size, low expression indicated poor prognosis PC. In vivo vitro experiments showed that stemness, apoptosis, proliferation cells. Furthmore, we also targeted by ALKBH5 for m6A modification, leading degradation YTHDF2. Bioinformatic analysis AS model based on TCGA database could target CD44 form different spliceosomes, thereby affecting biological behavior The conversion CD44s CD44V key Transcriptomic conducted reveal mechanism CELF2-mediated We led changes level endoplasmic reticulum stress, further regulating reticulum-associated (ERAD) signaling pathway, apoptosis cell stemness. addition, ERAD pathway inhibitor, EerI, effectively reverse effect tumors. This study indicates N6-methyladenosine-mediated promotes CD44, expected be new targeted-drug development.

Language: Английский

Citations

19
LncRNA CRNDE hinders the progression of osteoarthritis by epigenetic regulation of DACT1 DOI
Ziqi Zhang,

Pei Yang,

Chunsheng Wang

et al.

Cellular and Molecular Life Sciences, Journal Year: 2022, Volume and Issue: 79(8)

Published: July 8, 2022

Language: Английский

Citations

18
Oncogenic lncRNA BBOX1-AS1 promotes PHF8-mediated autophagy and elicits sorafenib resistance in hepatocellular carcinoma DOI Creative Commons
Haisu Tao, Yuxin Zhang, Jiang Li

et al.

Molecular Therapy — Oncolytics, Journal Year: 2022, Volume and Issue: 28, P. 88 - 103

Published: Dec. 23, 2022

Some long non-coding RNAs (lncRNAs) have been documented to be involved in cancer progression and anticancer drug resistance hepatocellular carcinoma (HCC). Thus, approaches designed target these genes may facilitate the development of promising strategies for treating HCC. Previously, we showed that lncRNA BBOX1-AS1 was highly expressed played an oncogenic role However, potential functions mechanisms through which regulates HCC remain unclear. This study revealed could promote tumor progression, autophagy, by upregulating PHF8 cells. Mechanistically, enhanced stability mRNA targeting inhibitor miR-361-3p regulate autophagy The functional rescue experiments acted as a key factor regulating biological effects induced HCC, indicating promotes sorafenib miR-361-3p/PHF8. Finally, mouse models patient-derived organoid were established further confirm findings. Taken together, results demonstrate via miR-361-3p/PHF8 axis.

Language: Английский

Citations

18
CELF2 inhibits bladder cancer progression by decreasing the stability of CXCL5 DOI
Shiqiang Dong, Lili Wang, Xinyu Liu

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: 370, P. 123585 - 123585

Published: March 28, 2025

Language: Английский

Citations

0
Comprehensive analysis of lncRNA-miRNA-mRNA ceRNA network and key genes in granulosa cells of patients with biochemical primary ovarian insufficiency DOI
Biting Liu, Li Liu,

Zubaidan Sulaiman

et al.

Journal of Assisted Reproduction and Genetics, Journal Year: 2023, Volume and Issue: 41(1), P. 15 - 29

Published: Oct. 17, 2023

Language: Английский

Citations

9