Communications Biology,
Journal Year:
2024,
Volume and Issue:
7(1)
Published: Nov. 12, 2024
Previous
reports
showed
that
long
non-coding
RNA
(lncRNA)
participates
in
the
development
and
progression
of
tumors.
Nevertheless,
effect
LINC02139
its
mechanism
on
gastric
cancer
(GC)
is
still
unknown.
We
revealed
upregulated
GC
cell
lines
tissues
high
expression
was
correlated
with
advancement
patients.
Functionally,
overexpression
promoted,
while
knockdown
impaired
proliferation,
migration,
invasion
vitro
impeded
tumorigenesis
a
tumor
xenograft
model
vivo.
Mechanistically,
directly
bound
to
XIAP
increased
protein
level
by
maintaining
stability
through
inhibition
ubiquitination
proteasome-dependent
degradation
pathway.
Importantly,
regulatory
function
LINC02139-mediated
oncogenic
effects
demonstrated.
Both
vivo
experiments
collaboratively
modulate
growth
apoptosis.
Analysis
clinical
further
confirmed
upregulation
positive
association
between
expression.
These
findings
established
as
driver
highlighted
crucial
involvement
LINC02139-XIAP
axis
progression,
suggesting
potential
promising
therapeutic
target
for
combating
advancement.
EMBO Reports,
Journal Year:
2023,
Volume and Issue:
24(12)
Published: Nov. 16, 2023
Abstract
Microbial
products,
such
as
lipopolysaccharide
(LPS),
can
elicit
efficient
innate
immune
responses
against
invading
pathogens.
However,
priming
with
LPS
induce
a
form
of
memory,
termed
“tolerance”,
which
blunts
subsequent
NF‐κB
signaling.
Although
epigenetic
and
transcriptional
reprogramming
has
been
shown
to
play
role
in
the
involvement
post‐translational
regulation
remains
unclear.
Here,
we
report
that
ubiquitin‐specific
protease
3
(USP3)
participates
establishing
“tolerance”
memory
through
non‐transcriptional
feedback.
Upon
signaling
activation,
USP3
is
stabilized
exits
nucleus.
The
cytoplasmic
specifically
removes
K63‐linked
polyubiquitin
chains
on
MyD88,
thus
negatively
regulating
TLR/IL1β‐induced
inflammatory
activation.
Importantly,
translocation
prerequisite
step
for
deubiquitinate
MyD88.
Additionally,
could
retention
faster
stronger
USP3,
enabling
it
quickly
shut
down
upon
second
challenge.
This
work
identifies
previously
unrecognized
feedback
loop
MyD88–USP3
axis,
critical
inducing
normal
memory.
BMC Medical Genomics,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: June 19, 2024
Abstract
Background
Immunoregulatory
drugs
regulate
the
ubiquitin-proteasome
system,
which
is
main
treatment
for
multiple
myeloma
(MM)
at
present.
In
this
study,
bioinformatics
analysis
was
used
to
construct
risk
model
and
evaluate
prognostic
value
of
ubiquitination-related
genes
in
MM.
Methods
results
The
data
on
MM
samples
were
downloaded
from
Cancer
Genome
Atlas
(TCGA)
Gene
Expression
Omnibus
(GEO)
databases.
consistent
cluster
ESTIMATE
algorithm
create
distinct
clusters.
constructed
through
single-factor
multiple-factor
analysis.
ROC
curve
plotted
compare
survival
difference
between
high-
low-risk
groups.
nomogram
validate
predictive
capability
model.
A
total
87
obtained,
with
47
showing
high
expression
group.
According
analysis,
4
clusters
determined.
immune
infiltration,
survival,
prognosis
differed
significantly
among
tumor
purity
higher
1
3
than
2
4,
while
score
stromal
lower
3.
proportion
B
cells
memory,
plasma
cells,
T
CD4
naïve
lowest
4.
KLHL24,
HERC6,
USP3,
TNIP1,
CISH
highly
expressed
high-risk
AICAr
BMS.754,807
exhibited
drug
sensitivity
group,
whereas
Bleomycin
showed
demonstrated
good
efficacy
predicting
patients
using
TCGA
GEO
datasets.
Conclusions
by
can
be
effectively
predict
patients.
warrant
further
investigation
as
therapeutic
targets
combat
resistance.
Cell Death Discovery,
Journal Year:
2024,
Volume and Issue:
10(1)
Published: May 21, 2024
Abstract
Proteins
are
the
keystone
for
execution
of
various
life
activities,
and
maintenance
protein
normalization
is
crucial
organisms.
Ubiquitination,
as
a
post-transcriptional
modification,
widely
present
in
organisms,
it
relies
on
sophisticated
ubiquitin-proteasome
(UPS)
system
that
controls
quality
modulates
lifespan.
Deubiquitinases
(DUBs)
counteract
ubiquitination
essential
homeostasis.
Ubiquitin
specific
peptidase
3
(USP3)
member
DUBs
has
received
increasing
attention
recent
years.
USP3
novel
chromatin
modifier
tightly
regulates
DNA
damage
response
(DDR)
maintains
genome
integrity.
Meanwhile,
acts
key
regulator
inflammatory
vesicles
sustains
normal
operation
innate
immune
system.
In
addition,
aberrantly
expressed
wide
range
cancers,
such
gastric
cancer,
glioblastoma
neuroblastoma,
implicating
could
be
an
effective
target
targeted
therapies.
this
review,
we
retrace
all
current
researches
USP3,
describe
structure
elucidate
its
functions
damage,
responses
cell
cycle,
summarize
important
role
multiple
cancers
diseases.
Aging,
Journal Year:
2024,
Volume and Issue:
16(11), P. 9485 - 9497
Published: May 30, 2024
Background:
Esophageal
squamous
cell
carcinoma
(ESCC)
is
a
gastrointestinal
malignancy
with
high
incidence.
This
study
aimed
to
reveal
the
complete
circRNA-miRNA-mRNA
regulatory
network
in
ESCC
and
validate
its
function
mechanism.
Method:
Expression
of
OTU
Domain-Containing
Ubiquitin
Aldehyde-Binding
Protein
2
(OTUB2)
was
analyzed
by
bioinformatics
find
binding
sites
between
circRNA6448-14
miR-455-3p,
as
well
miR-455-3p
OTUB2.
The
relationships
were
verified
RNA
Immunoprecipitation
(RIP)
dual-luciferase
assay.
expressions
circRNA6448-14,
OTUB2
detected
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR).
MTT
assay
measured
viability,
spheroid
formation
assessed
ability
stem
sphere
formation.
Western
blot
(WB)
determined
expression
marker
proteins
surface
rate-limiting
enzyme
glycolysis.
Seahorse
XFe96
extracellular
flux
analyzer
rate
acidification
cellular
oxygen
consumption.
Corresponding
kits
glucose
consumption,
lactate
production,
adenosine
triphosphate
(ATP)
generation.
Results:
In
ESCC,
highly
expressed
contrast
miR-455-3p.
Knocking
down
could
prevent
glycolysis
stemness
cells.
Additionally,
enhanced
sponging
Overexpression
or
silencing
reversed
inhibitory
effect
knockdown
on
stemness.
Conclusion:
research
demonstrated
that
circRNA6448-14/miR-455-3p/OTUB2
axis
induced
Our
revealed
novel
which
may
serve
potential
therapeutic
target
for
ESCC.
Communications Biology,
Journal Year:
2024,
Volume and Issue:
7(1)
Published: Nov. 12, 2024
Previous
reports
showed
that
long
non-coding
RNA
(lncRNA)
participates
in
the
development
and
progression
of
tumors.
Nevertheless,
effect
LINC02139
its
mechanism
on
gastric
cancer
(GC)
is
still
unknown.
We
revealed
upregulated
GC
cell
lines
tissues
high
expression
was
correlated
with
advancement
patients.
Functionally,
overexpression
promoted,
while
knockdown
impaired
proliferation,
migration,
invasion
vitro
impeded
tumorigenesis
a
tumor
xenograft
model
vivo.
Mechanistically,
directly
bound
to
XIAP
increased
protein
level
by
maintaining
stability
through
inhibition
ubiquitination
proteasome-dependent
degradation
pathway.
Importantly,
regulatory
function
LINC02139-mediated
oncogenic
effects
demonstrated.
Both
vivo
experiments
collaboratively
modulate
growth
apoptosis.
Analysis
clinical
further
confirmed
upregulation
positive
association
between
expression.
These
findings
established
as
driver
highlighted
crucial
involvement
LINC02139-XIAP
axis
progression,
suggesting
potential
promising
therapeutic
target
for
combating
advancement.
