Identification of a novel signature derived from ferroptosis-related genes to predict prognosis, immune landscape and chemotherapeutic sensitivity in head and neck squamous cell carcinoma

Journal of Stomatology Oral and Maxillofacial Surgery, Journal Year: 2025, Volume and Issue: unknown, P. 102392 - 102392

Published: April 1, 2025

Language: Английский

---

Proteomics and phosphoproteomics revealed dysregulated kinases and potential therapy for liver fibrosis

Molecular & Cellular Proteomics, Journal Year: 2025, Volume and Issue: unknown, P. 100991 - 100991

Published: May 1, 2025

Language: Английский

---

Autophagy and exosome dynamics in Radiation-Induced pulmonary fibrosis: the critical role of TRIB3

Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)

Published: May 21, 2025

Dysregulated autophagy plays a critical role in the pathogenesis of pulmonary fibrosis. The stress protein TRIB3 has been correlated with abnormal autophagy, but its specific contribution to radiation-induced fibrosis (RIPF) remains unclear. This study aimed elucidate RIPF progression. We conducted RNA-sequencing rat lung tissue analyze transcriptomic profile and determine gene expression changes murine RIPF. established mouse models alveolar epithelial type II cells (AEC II)-specific knockdown or overexpression utilized mRFP-GFP-LC3 fluorescent reporter cells, nanoparticle tracking analysis, immunofluorescence immunoprecipitation assays uncover underlying mechanisms. was elevated irradiated AEC II. Silencing mitigated mice, whereas exacerbated condition. Mechanistically, interacted LC3-interacting region (LIR) motif ubiquitin-associated (UBA) domain sequestosome 1 (SQSTM1), an autophagic receptor protein, thereby inhibiting flux cell line MLE12. inhibition increased exosome secretion facilitated crosstalk between MLE12 fibroblasts, ultimately enhancing proliferation extracellular matrix production fibroblasts. inhibits by interacting SQSTM1, increasing secretion, which promotes fibroblast production, contributing

Language: Английский

---

Thirdhand smoke exposure promotes gastric tumor development in mouse and human

Environment International, Journal Year: 2024, Volume and Issue: 191, P. 108986 - 108986

Published: Aug. 26, 2024

The pollution of indoor environments and the consequent health risks associated with thirdhand smoke (THS) are increasingly recognized in recent years. However, carcinogenic potential THS its underlying mechanisms have yet to be thoroughly explored. In this study, we examined effects short-term exposure on development gastric cancer (GC) vitro vivo. a mouse model spontaneous GC, CC036, observed significant increase tumor incidence decrease tumor-free survival upon as compared control. RNA sequencing primary epithelial cells derived from CC036 mice showed that increased expression genes related extracellular matrix cytoskeletal protein structure. We then identified exposure-induced 91-gene signature homologous 84-gene human GC patients predicted prognosis, secreted phosphoprotein 1 (SPP1) tribbles pseudokinase 3 (TRIB3) emerging targets through which may promote carcinogenesis. also treated cell lines media containing various concentrations THS, which, some dose range, significantly their proliferation, invasion, migration. could activate epithelial-mesenchymal transition (EMT) pathway at transcript level. conclude is an risk activation EMT program one mechanism. Increased understanding will help identify new preventive therapeutic strategies for tobacco-related disease well provide scientific evidence rationale policy decisions control protect vulnerable subpopulations such children.

Language: Английский

---

Leveraging programmed cell death patterns to predict prognosis and therapeutic sensitivity in OSCC

Oral Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Objectives Intricate associations between programmed cell death (PCD) and cancer development treatment outcomes have been increasingly appreciated. Here, we integrated 12 PCD patterns to construct a novel biomarker, index (CDI), for oral squamous carcinoma (OSCC) prognostication therapeutic prediction. Materials Methods Univariate Cox regression, Kaplan–Meier survival, LASSO analyses were performed the CDI. A nomogram combining CDI selected clinicopathological parameters was established by multivariate regression. The immune landscape sensitivity estimated. Single‐cell RNA‐seq data of OSCC used infer genes in types determine their expression along differentiation trajectory. Results Ten derived prognostic signature OSCC. predictive performance robust superior across multiple independent patient cohorts. negatively associated with tumor‐infiltrating abundance immunotherapeutic outcomes. Moreover, scRNA‐seq reanalysis revealed that GSDMB, IL‐1A, PRKAA2, SFRP1 from this primarily expressed cells involved differentiation. Conclusions Our findings as powerful predictor prognosis response suggested its potential involvement

Language: Английский

---

Comprehensive Bioinformatics Analysis Reveals the Potential Role of the hsa_circ_0001081/miR-26b-5p Axis in Regulating COL15A1 and TRIB3 within Hypoxia-Induced miRNA/mRNA Networks in Glioblastoma Cells

Biomedicines, Journal Year: 2024, Volume and Issue: 12(10), P. 2236 - 2236

Published: Oct. 1, 2024

The intrinsic molecular heterogeneity of glioblastoma (GBM) is one the main reasons for its resistance to conventional treatment. Mesenchymal GBM niches are associated with hypoxic signatures and a negative influence on patients' prognosis. To date, competing endogenous RNA (ceRNA) networks have been shown broad impact progression various cancers. In this study, we decided construct hypoxia-specific microRNA/ messengerRNA (miRNA/mRNA) putative circular (circRNA) regulatory component using available bioinformatics tools.

Language: Английский

---