Egyptian Journal of Medical Human Genetics,
Journal Year:
2024,
Volume and Issue:
25(1)
Published: Oct. 12, 2024
Abstract
Background
Endometriosis
is
a
common
cause
of
endometrial-type
mucosa
outside
the
uterine
cavity
with
symptoms
such
as
painful
periods,
chronic
pelvic
pain,
pain
intercourse
and
infertility.
However,
early
diagnosis
endometriosis
still
restricted.
The
purpose
this
investigation
to
identify
validate
key
biomarkers
endometriosis.
Methods
Next-generation
sequencing
dataset
GSE243039
was
obtained
from
Gene
Expression
Omnibus
database,
differentially
expressed
genes
(DEGs)
between
normal
control
samples
were
identified.
After
screening
DEGs,
gene
ontology
(GO)
REACTOME
pathway
enrichment
analyses
performed.
Furthermore,
protein–protein
interaction
(PPI)
network
constructed
modules
analyzed
using
Human
Integrated
Protein–Protein
Interaction
rEference
database
Cytoscape
software,
hub
Subsequently,
miRNAs
genes,
TFs
miRNet
NetworkAnalyst
tool,
possible
predicted.
Finally,
receiver
operating
characteristic
curve
analysis
used
genes.
Results
A
total
958
including
479
upregulated
downregulated
screened
samples.
GO
DEGs
showed
that
they
mainly
involved
in
multicellular
organismal
process,
developmental
signaling
by
GPCR
muscle
contraction.
Further
PPI
identified
10
vcam1,
snca,
prkcb,
adrb2,
foxq1,
mdfi,
actbl2,
prkd1,
dapk1
actc1.
Possible
target
miRNAs,
hsa-mir-3143
hsa-mir-2110,
TFs,
tcf3
(transcription
factor
3)
clock
(clock
circadian
regulator),
predicted
constructing
miRNA-hub
regulatory
TF-hub
network.
Conclusions
This
bioinformatics
techniques
explore
potential
novel
biomarkers.
These
might
provide
new
ideas
methods
for
diagnosis,
treatment
monitoring
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(21), P. 11803 - 11803
Published: Nov. 2, 2024
Low
back
pain
(LBP)
is
a
significant
global
health
issue,
contributing
to
disability
and
socioeconomic
burdens
worldwide.
The
degeneration
of
the
human
intervertebral
disc
(IVD)
critical
factor
in
pathogenesis
LBP.
Recent
studies
have
emphasized
significance
specific
set
genes
extracellular
matrix
(ECM)
IVD
health.
In
particular,
Noggin
has
emerged
as
gene
due
its
high
expression
levels
healthy
nucleus
pulposus
cells
(NPCs)
observed
our
previous
research.
this
study,
it
was
hypothesized
that
decreased
NPCs
associated
with
contributes
LBP
development.
A
lentivirus-mediated
RNAi
applied
knock
down
primary
from
six
donors.
after
transduction
were
evaluated
through
cell
viability
analysis,
XTT
assay,
apoptosis
analyses.
After
two
weeks,
colony
formation
assay
used
examine
anchor-independent
growth
ability
transduced
cells.
At
transcript
level,
anabolic
catabolic
markers
quantified
using
RT-qPCR.
results
demonstrated
downregulation
significantly
inhibited
proliferation,
reduced
viability,
suppressed
formation,
while
inducing
vitro.
Notably,
disrupted
cellular
processes
promoted
activity
post-transduction.
Our
findings
indicated
possibly
related
NPCs.
This
research
provides
valuable
insights
into
role
homeostasis
implications
treatment.
Poultry Science,
Journal Year:
2024,
Volume and Issue:
104(2), P. 104761 - 104761
Published: Dec. 31, 2024
For
commercial
laying
hens,
the
continuous
high-intensity
ovulation
process
leads
to
a
significant
accumulation
of
reactive
oxygen
species
(ROS)
in
granulosa
cells,
inducing
oxidative
stress,
which
accelerates
ovarian
aging
and
shortens
peak
period.
The
molecular
mechanisms
underlying
this
remain
poorly
understood.
Therefore,
we
modeled
processes
stress
antioxidant
chicken
cells.
Small
RNA
sequencing
revealed
that
miR-15c-3p
expression
was
elevated
by
induction
attenuated
curcumin.
Functional
validation
with
mimic
inhibitor
confirmed
role
exacerbating
resultant
suppression
lipid
droplet
storage
progesterone
secretion
cells
targeting
insulin-like
growth
factor
2
binding
protein
3
(IGF2BP3).
These
regulatory
effects
were
mediated
through
sequential
downstream
signaling
cascade
AKT-Raf1-ERK1/2.
In
conclusion,
IGF2BP3
curbed
restores
disrupted
under
AKT-Raf1-ERK1/2
pathway.
findings
offer
new
insights
into
damages
reproductive
capacity
theoretical
basis
for
mitigating
hens
genetic
improvement.
Egyptian Journal of Medical Human Genetics,
Journal Year:
2024,
Volume and Issue:
25(1)
Published: Oct. 12, 2024
Abstract
Background
Endometriosis
is
a
common
cause
of
endometrial-type
mucosa
outside
the
uterine
cavity
with
symptoms
such
as
painful
periods,
chronic
pelvic
pain,
pain
intercourse
and
infertility.
However,
early
diagnosis
endometriosis
still
restricted.
The
purpose
this
investigation
to
identify
validate
key
biomarkers
endometriosis.
Methods
Next-generation
sequencing
dataset
GSE243039
was
obtained
from
Gene
Expression
Omnibus
database,
differentially
expressed
genes
(DEGs)
between
normal
control
samples
were
identified.
After
screening
DEGs,
gene
ontology
(GO)
REACTOME
pathway
enrichment
analyses
performed.
Furthermore,
protein–protein
interaction
(PPI)
network
constructed
modules
analyzed
using
Human
Integrated
Protein–Protein
Interaction
rEference
database
Cytoscape
software,
hub
Subsequently,
miRNAs
genes,
TFs
miRNet
NetworkAnalyst
tool,
possible
predicted.
Finally,
receiver
operating
characteristic
curve
analysis
used
genes.
Results
A
total
958
including
479
upregulated
downregulated
screened
samples.
GO
DEGs
showed
that
they
mainly
involved
in
multicellular
organismal
process,
developmental
signaling
by
GPCR
muscle
contraction.
Further
PPI
identified
10
vcam1,
snca,
prkcb,
adrb2,
foxq1,
mdfi,
actbl2,
prkd1,
dapk1
actc1.
Possible
target
miRNAs,
hsa-mir-3143
hsa-mir-2110,
TFs,
tcf3
(transcription
factor
3)
clock
(clock
circadian
regulator),
predicted
constructing
miRNA-hub
regulatory
TF-hub
network.
Conclusions
This
bioinformatics
techniques
explore
potential
novel
biomarkers.
These
might
provide
new
ideas
methods
for
diagnosis,
treatment
monitoring
